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1992 Fiscal Year Final Research Report Summary

Molecular cytogenetic studies on tumorigenic genetic changes in bone and soft tissue malignancies

Research Project

Project/Area Number 02454339
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionHokkaido University

Principal Investigator

ABE Syuiti  Hokkaido Univ., Fac. Sci., Assoc. Prof., 理学部, 助教授 (80125278)

Co-Investigator(Kenkyū-buntansha) NOJIMA Takayuki  Hokkaido Univ., Sch. Med., Lectur., 医学部, 講師 (50142732)
MATSUNO Takeo  Hokkaido Univ., Sch. Med., Assoc.Prof., 医学部, 助教授 (10165847)
YOSHIDA Michihiro  Hokkaido Univ., Fac. Sci., Prof., 理学部, 教授 (60001765)
Project Period (FY) 1990 – 1992
KeywordsMolecular cytogenetics / Bone and soft tissue malignancies / Rhabdomyosarcoma / Ewing's sarcoma / Chromosome translocation / Breakpoint / Gene rearrangement / RFLPs
Research Abstract

Seeking for the genetic changes involved in the pathogenesis of malignant bone and soft tissue tumors, molecular cytogenetic studies were performed for rhabdomyo- sarcoma (RMS) and Ewing's sarcoma (ES). The results obtained are summarized below. 1. To overcome infrequent occurrence of RMS and ES, we successfully established 16 four ES cell lines in nude mice and/or in culture. 2. Chromosome analysis on tumor cells from RMS and ES patients revealed that t(2;13)(q35-q37;q14) and t(11;22)(q23;q12) are specific for alveolar RMS and ES, respectively. 3. No rearrangement of the 13 genes (D2S3, FN1, MYL1-3, ALPP, COL6A3, APOA1, APOA4, APOC3, D13S1, RB1, ESD, PDGFB, and NEFH) localizing at or near the breakpoints of t(2;13) and t(11;22) was detected by Southern blot analysis on DNA from tumor cells with or without the specific translocations, although FLI1 at 11q23 and EWS at 22q12 were shown to be rearranged in the t(11;22), in agreement with the recent report. RT-PCR with cDNA synthesized from tumor cell RNA revealed a novel break- point of t(11;22) in one of the ES cases. 4. The t(2;13) breakpoints were shown to be located between FN1 and COL6A3 on chromosome 2 and between D13S1 and RB1 on chromosome 13 by Southern blot and chromosomal in situ hybridization analyses. 5. Whereas RFLPs were rare for the above 15 genes, frequent RFLPs were detected at several VNTR loci on chromosomes 1,2,13,17,and 22 in RMS and ES cases examined.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takayuki Nojima: "A case of alveolar rhabdomyosarcoma with a chromosome translocation,t(2;13)(q37;q14)" Virchows Archiv A Pathological Anatomy and Histopathology. 417. 357-359 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Nojima: "Morphological and cytogenetic studies of a human synovial sarcoma xenotransplanted into nude mice" Acta Pathologica Japonica. 40. 486-493 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeo Matsuno: "Benign fibrous histiocytoma involving the ends of long bone" Skeletal Radiology. 19. 561-566 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masayoshi Iizuka: "Joining of the c-myc gene and a line l family member on chromosome 8 in a human primary giant cell tumor of lung" Cancer Research. 50. 3345-3350 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Nojima: "Morphological and cytogenetic characterization and N-myc oncogene analysis of a newly established neuroblastoma cell line" Acta pathologica Japonica. 41. 507-515 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xing Mao: "Chromosome banding by restriction enzyme aluI and its buffer" Chromosome Information Service. 50. 15-16 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tadao Arinami: "Chromosomal assignment of the human endothelin family genes:the endothelin-1 gene (EDN1)to 6p23-p24,the endothelin-2 gene (EDN2)to 1p34,and the endothelin-3 gene(EDN3)to 20q13.2-q13.3" American Journal of Human Genetics. 48. 990-996 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi Sonobe: "Establishment and characterization of a new human synovial sarcoma cell line,HS-SY-II" Laboratory Investigation. 67. 498-505 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Nojima: "Osteosarcoma resembling osteoblastoma and its heterotransplantation into nude mice" Acta Pathologica Japaonica. 42. 75-81 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nanao Kamada: "Chromosome Abnormalities in adult T-cell leukemia/lymphoma:a karyotype review committee report" Cancer Research. 52. 1481-1493 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Michihiro C.Yoshida: "Recent advances in fluorescence in situ hybridization" Journal of Radiation Research(Supplement). 33. 54-60 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 阿部 周一: "上皮性分化を示す骨・軟部悪性腫瘍の染色体異常" 病理と臨床. 10. 1385-1390 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeo Matsuno: "Trends in Research and Treatment of Joint Diseases" Springer-Verlag,Tokyo, 182 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takayuki Nojima et al.: "A case of alveolar rhabdo- myosarcoma with a chromosome translocation, t(2;13) (q37;q14)" Virchows Archiv. A Pathol. Anat.417. 357-359 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayuki Nojima et al.: "Morphological and cytogenetic studies of a human synovial sarcoma xeno- transplanted into nude mice" Acta Pathol. Jpn. 40. 486-493 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Matsuno: "Benign fibrous histiocytoma involving the ends of long bone" Skeletal Radiol.19. 561-566 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masayoshi Iizuka et al.: "Joining of the c-myc gene and a line 1 family member on chromosome 8 in a human primary giant cell tumor of lung" Cancer Res.50. 3345-3350 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayuki Nojima et al.: "Morphological and cytogenetic characterization and N-myc oncogene analysis of a newly established neuroblastoma cell line" Acta Pathol. Jpn.41. 507-515 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xing Mao et al.: "Chromosome banding by restriction enzyme AluI and its buffer" Chrom. Inform. Serv.50. 15-16 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tadao Arinami et al.: "Chromosomal assignment of the human endothelin family genes: the endothelin- l gene (EDN1) to 6p23-p24, the endothelin-2 gene (EDN2) to lp34, and the endothelin-3 gene (EDN3) to 20q13.2-q13.3" Am. J. Hum. Genet.48. 990-996 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi Sonobe et al.: "Establishment and characterization of a new human synovial sarcoma cell line, HS-SY-II" Lab. Invest.67. 498-505 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayuki Nojima et al.: "Osteosarcoma resembling osteoblastoma and its heterotransplantation into nude mice" Acta Pathol. Jpn.42. 75-81 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nanao Kamada et al.: "Chromosome abnormalities in adult T-cell leukemia/lymphoma: a karyotype review committee report" Cancer Res.52. 1481-1493 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Michihiro C. Yoshida: "Recent advances in fluores- cence in situ hybridization" J. Radiat. Res.33. 54-60 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Syuiti Abe et al: "Chromosome abnormalities in bone and soft tissue malignancies with epithelial phenotype (in Japanese)" Pathol. Clin. Med.10. 1385-1390 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Matsuno et al.: "Trends in Research and Treatment of Joint Diseases" Springer-Verla, Tokyo. 1-182 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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