1991 Fiscal Year Final Research Report Summary
Development of new tumor markets for prostate cancer
| Project/Area Number |
02454367
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Gunma University |
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Principal Investigator |
YAMANAKA Hidetoshi Gunma University School of Medicine Dept. Urol. Professor, 医学部, 教授 (70110393)
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| Project Period (FY) |
1990 – 1991
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| Keywords | EMBP / Prostate cancer / Tumor marker / Metallothionein |
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| Research Abstract |
Most of prostate cancer are adenocarcinoma, which possess the secretory function and secrete many kinds of proteins as well as normal prostate. As the prostate cancer has no functional ductal system, the proteins secrete from prostate cancer cells and leak into the blood vessel. The serum concentration of some prostate secretory proteins are thought to be in proportion to the volume of the prostate cancer mass. EMBP (estramustine binding protein) and metallothionein have been ascertained to be secretory proteins of prostate by us. Therefore, We examined whether EMBP and metallothionein possess the possibility of tumor marker for the prostate cancer. We established the more speedy and simpler preparative method of rat prostate EMBP than the existing one. We clarified by means of the western blotting method that the polyclonal antibody recognized all 3 components (C1, C2 and C3) of EMBP in both the ventral prostate and the dorsolateral prostate. However, this polyclonal antibody could not recognized human prostate EMBP. And then, We tried the preparation of monoclonal antibodies against rat prostate EMBP. Fortunately, we obtained two monoclonal antibodies that rcognized the human prostate EMBP.
We investigated prostate metallothionein. And we were the first to clarify the existance of metallothionein in the human prostate by means of the immunohistochemical detection.
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[Publications] Suzuki, T., Umeyama, T., Ohma, C., Yamanaka, H., Suzuki, K., Nakajuma, K. and Kimura, M.: "Immunohistochemical study of metallothinein in normal and benign normal and benign prostatic hyperplasia of human prostate." Prostate. 19. 35-42 (1991)
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[Publications] Suzuki, T., Yamanaka, H., Tamura, Y., Nakajima, K., Kanatani, K., Kimura, M. and Otaki, N.: "Metallothionein of prostatic tissues and fluids in rat and human" Tohoku J. Exp. Med. (1992)
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[Publications] Kobayashi, H., Sasaki, Y., Hayashi, M., Yamanaka, H., Inoue, T. and Igarashi, H.: "Evaluation and clinical application of RIA kit for prostate specific antigen." Igaku to Yakugaku. 23. 367-373 (1990)
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