1991 Fiscal Year Final Research Report Summary
Improvement and application of high-vacuum evaporater with condensed-light beam heater for deep-etching replication of TEM specimen
| Project/Area Number |
02559005
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Yamagata University |
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Principal Investigator |
TONOSAKI Akira Yamagata Univ. School of Med., Professor, 医学部, 教授 (90004572)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Kazutomo Yamagata Univ. School of Med., Instructor, 医学部, 助手 (80234657)
WATANABE Hiroshi Yamagata Univ. School of Med., Associate Prof., 医学部, 助教授 (80004662)
AITA Saburo JEOL Co. Ltd., Head, 研究室長
YAGIHASHI Soroku Hirosaki Univ. Sch. of Med., Assistant Prof., 医学部, 講師 (40111231)
TOKUNAGA Fumio Osaka Univ. School of Med., Professor, 理学部, 教授 (80025452)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Clean-vacuum evaporater / Freeze-fracturing replica / Deep-etching replica / Surface-sublimation by condensed-light beam / Biological membranes |
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| Research Abstract |
A high-vacuum evaporater has been proposed and completed in the form of JFD-9010S (JOEL) by this project. It is characterized by 'clean vecuum' with a turbomolecular and a cryo-absorption pump, keeping out the traditional oil-diffusion pump. For facilitating the deep-etching or sublimation process on the fractered surface of the specimen maintained at the low-temperature stage, a condensed-light heating device has been adopted. Using this innovated device, adequate deep-etching is obtained after 40-60 min irradiation during which the specimen is maintained at -110 to -120ﾟC, limiting the sublimation to the very surface to be replicated.
Application to the biological membrane structure, including of photoreceptor cells, halobacteria, and diabetic neural fibers, is going on. The final data will be quatitatively analyzed with the special soft-ware arranged for counting the intramembrane particles and other deep-etching fine structures on TEM pictures projected to CRT.
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