1991 Fiscal Year Final Research Report Summary
Study on the function of gap junctions by using the monoclonal antibodies against gap junction proteins
| Project/Area Number |
02670038
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Hiroshima University |
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Principal Investigator |
SUGITA Makoto Hiroshima University School of Dentistry Research Associate, 歯学部, 助手 (50235884)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBA Yoshiki Hiroshima University School of Dentistry Associate Professor, 歯学部, 助教授 (90110452)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Gap junction / Intercellular commumication / Salivary gland / Monoclonal antibody / Immunohistochemistry |
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| Research Abstract |
In this project, we have been using the monoclonal antibodies against the gap Junction proteins to clarify the function of gap junctions. We used the monoolonal antibodies against the polypeptides identloal to the gap Junction proteins in the liver for the immunohistochemical staining and immunoblotting.
The gap Junction proteins in the liver consisted of two different proteins ; 21k and 27k proteins, although the composition ratio of both proteins was different in the species of the animals. The gap-junctional intercellular communication in the cell lines derived from the liver was detected by using the fluorescent dye-transfer technique, but the gap Junotion proteins were not detected by both the immunohistochemioal and immunoblotting methods. the expression of gap junotion proteins in the hepatocytes might be modulated by the enviromental conditions.
The main proteins of the gap junotions in the aoinar cells of rat salivary gland consisted of 27k gap junction protein. The 21k gap junction protein was also detected in the acinar oells and in some portion of interoallated ducts. The cholinergic agonlsts suppressed the gap-Junotional intercellular communioation in the acinar cells of rat submandibular glands. The funotion of 27k gap junotion proteins might be regulated by the parasympathetic nerve and might play some role in control of salivary secretion.
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