1991 Fiscal Year Final Research Report Summary
Delayed Neurotoxicity of Organophosphorus Compound
| Project/Area Number |
02670226
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Fukushima Medical College |
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Principal Investigator |
KONNO Nobuhiro Fukushima Medical College, Dept. of Public Health Assistant Professor, 医学部, 講師 (10045784)
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| Co-Investigator(Kenkyū-buntansha) |
KATOH Kiyoshi Fukushima Medical College, Dept. of Public Health Instructor, 医学部, 助手 (40136974)
YAMAUCHI Toru Mie University School of Medicine, Dept. of Public Health Professor, 医学部, 教授 (80045674)
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| Project Period (FY) |
1990 – 1991
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| Keywords | Organophosphate / DFP / Delayed Neurotoxicity / Chicken / OPIDN / Diisopropylfluorophosphate |
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| Research Abstract |
Binding of [^3H]Diisopropylfluorophosphate(DFP) to membranes from the chicken spinal cord has been studied. [^3H]DFP binding was specific, saturable and reversible. [^3H]DFP binding sites behaved like a receptor site(s). Scatchard plots revealed two classes of binding sites. The competition curves showed that [^3H]DFP binding was inhibited by the delayed neurotoxic compound, such as DFP, mipafox and leptophos. It was also inhibited by paraoxon. Paraoxon might act as an antagonist to the binding sites. Autoradiography of [^3H]DFP binding in spinal cord slices showed higher levels of binding sites in gray matter compared with white matter. A single iv dosing of 40 mg/kg of tri-o-cresyl phosphate (TOCP) to chickens resulted in more than 70% decrease of [^3H]DFP binding sites in spinal cord membranes after 6 to 24 hr. This suggests that [^3H]DFP binding sites might be involved in the initial target of organophosphate induced delayed neurotoxicity (OPIDN).
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Research Products
(11 results)
