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1992 Fiscal Year Final Research Report Summary

RESEARCH FOR TRANSMEMBRANE SIGNALING SYSTEM OF EPIDERMAL KERATINOCYTES; INTRACELLULAR FREE CALCIUM AND CELLULAR RESPONSES.

Research Project

Project/Area Number 02670469
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

KOIZUMI Hiroko  HOKKAIDO UNIVERSITY MEDICAL HOSPITAL, DERMATOLOGY, LECTURER, 医学部附属病院, 講師 (60113552)

Co-Investigator(Kenkyū-buntansha) YASUI Chikako  HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (50210240)
FUKAYA Toru  HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (90133727)
Project Period (FY) 1990 – 1992
KeywordsKERATINOCYTE / CALCIUM / PEROXIDE / SUBSTANCE P / PHOSPHOLIPASE C / PROTEIN KINASE C / ADENYLATE CYCLASE / PSORIASIS
Research Abstract

Epidermal keratinocytes proliferate and differentiate to make stratum corneum. Many chemical mediators, hormones and peptides stimulate keratinocytes to induce phosphophatidylinositol metabolism, alteration of intracellular free calcium, adenylate cyclase response activation. We elucidated that bradykinin, thrombin, substance P, beta-adrenarin, adenosine, and histamine induce increase of intracellular free calcium. These effects are mediated with synthesis of inositol 1,4,5-trisphosphate or adenylate cyclase activation. The importance of calcium as a second messenger is shown. Presence of membrane skeleton proteins such as spectrin, b-fodrin, 4.1 protein like proteins were detected immunologically and the alteration of their distribution in cultured keratinocytes were observed with the change of calcium concentration of the medium. A new technique was reported to detect peroxide produced from keratinocytes using dihydrorhodamine 123.
Cross-talk among transmembrane signaling systems in keratinocytes, and drugs to influence these systems should be investigated. Abnormal keratinization and acceleration of proliferation are considered to closely realt to pathogenesis of psoriasis. The alteration of membrane signaling systems in psoriasis and the action mechanisms of the drugs used in therapy of skin diseases are under investigation.

  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] KOIZUMI H.: "Substance P induces intracellular Ca^<++>increase and protein kinase C avtivation in the epidermis." British Journal of Dermatology. 127. 595-599 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOIZUMI H.: "Effect of novel synthesized retino-benzoic acid(Am-80)on adenylate cyclase and arachidonic release of pig epidermis." Journal of Dermatological Science. 3. 97-102 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YASUI C: "Adenylate cyclase induces intracellular calcium increase measured by imaging fluorography using Fura2-AM." British Journal of Dermatology. 127. 589-594 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAKURADA H.: "Use of dihydrorhodamine123for detecting intracellular generation of peroxide upon UV irradiation in epidermal keratinocytes." Archives of Dermatological Research. 284. 114-116 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHIMIZU T.: "Localization of proteins immunologically related to erythrocyte protein4.1,spectrin and ankyrin thyroid gland." Acta Histochemica. 93. 441-445 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOIZUMI H.: "Adenosine deaminase activity in serum of patients with psoriasis,mycosis fungoides,and adult T ccll leukemia." Acta Dermatovenereologica(Stockh). 72. 410-412 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "表皮細胞情報伝達系とカルシウム." 皮膚診療. 14. 402-405 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "角化異常とレチノイド." FRAGRANCE JOURNAL.20. 26-31 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 飯塚 一: "表皮細胞における核酸の生合成と代謝." FRAGRANCE JOURNAL.20. 41-47 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "neuropeptide." 皮膚科の臨床.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "Protein kinase C." 皮膚科の臨床.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "情報伝達系" 日本皮膚科学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KOIZUMI H.: "clinical significance of serum adenosine deaminase activity in patients with mycosis fungoides." Journal of Dermatology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHIMIZU T.: "Calcium dependent formation of 4.1-like protein in cultured human keratinocytes." Histochemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHIMIZU T.: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Arch Derm Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] OSADA S.: "predominant expression of nPKCη,aCa^<2+>-independent isoform of protein kinase C in epithelial tissues,in association with epithelial differentiation." Cell Growth and Differentiation.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小泉 洋子: "乾癬.Today's therapy1993今日の治療指針" 医学書院, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koizumi H, Fukaya T, Tanaka H, Ohkawara A,Ueda T:"Substance Pinduces intracellular calicium increase and protein kinase C activation in the epiudermis." British Journal of Dermatology. 127. 595-599 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H, Sato KC, Ohkawara A: "Effect of novel synthesized retinobenzoic acid (Am-80) on adenylate cyclase and arachidonic acid release of pig epiudermis." Journal of Dermatological Research. 3. 97-102 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yasui C, Koizumi H, Fukaya T, Ohkawara A, Ueda T:"Adenylate cyclase induces intracellular calcium increase measured by imaging fluorography using Fura 2-AM." British Journal of Dermatology. 127. 589-594 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakurada H, Koizumi H, Ohkawara A, Ueda T, Kamo N: "Use of dihydrorhodamin 123 for detecting intracellula generation of peroxide upon UV irradiation in epidermal keratinocytes." Archives of Dermatological Research. 284. 114-116 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Localization of proteins immunologically related to erythrocyte protein 4.1, spectrin and ankyrin n thyroid gland." Acta Histochemica. 93. 441-445 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H, Ohkawara A: "Adenosine deaminase activity in serum of patients with psoriasis, mycosis fungoides, and adult T cell leukemia." Acta Dermatovenereologica(Stockh). 72. 410-412 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H: "Signal transduction and calcium in epidermal keratinocytes." Hihu Shinryo. 14. 402-405 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi Hiroko: "Disorder of keratinization and retinoid" Fragrance Journal. 20. 26-31 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iizuka H:"Nucleic acid metabolism in epidermal keratinocytes" Fragrance Journal. 20. 41-47 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H: "Neuropeptide" Hihu Rinshou.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H: "Protein kinase C" Hihu Rinshou.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H: "signal transduction in epidermal keratinocytes." Japanese Journal of Dermatology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H, Tomizawa K, Tanaka H, Kumakiri M, Ohkawara A: "Clinical significance of serum adenosine deaminase activiy in patients with mycosis fungoides." Journal of Dermatology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Calcium dependent formation of 4.1-like protein in cultured human keratinocytes." Histochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu T, Takakuwa Y, Koizumi H, Ishibashi T, Ohkawara A: "Immunoreactive analogues of erythrocyte ankyrin in human epidermal keratinocyte." Archives of Dermmatological Research.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] OsadaS,Hashimoto Y, Nomura S, Kohno Y, Chida K, Tajima O, Kubo K, Akimoto K, Koizumi H, Kitamura S, Suzuki K, Ohno S, Kuroki T: "Predominant expression of nPKCeta, a Ca^<2+>independent isoform of protein kinase C in epithelial tissues, in association with epithelial differentiation." Cell Growth and Differentiation.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koizumi H: "psoriasis" Today's therapy 1993.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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