1992 Fiscal Year Final Research Report Summary

Sensitivity test of anticancer agents using 31P NMP Spectroscopy

Research Project

Project/Area Number	02670539
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General surgery
Research Institution	Nagoya University
Principal Investigator	AKIYAMA Seiji Nagoya University Assistant Professor, 医学部, 助手 (40202551)
Co-Investigator(Kenkyū-buntansha)	TAKAGI Hiroshi Nagoya University Professor, 医学部, 教授 (70154755)
Project Period (FY)	1990 – 1992
Keywords	31P NMR Spectroscopy / sensitivity test / anticancer agent / chemotherapy
Research Abstract	1) in vitro Experiment Using human gastric cancer cell lines, the metabolism of phospholpids was examined after treatment of anticancer agents. Too much number of cancer cells were needed, and the pek of 31P NMP spectrum was not cleat. The sensitivity of anticancer agents was not clearly observed in this manner. 2) in vivo Experiment Human gastric cancer cell line, NU-GC-4 was implanted in the subcutaneons space of nude mice. CDDP (10mg, 7.5mg, 5mg/Kg) was administered intraperotoneally at 7th day, and phospholipid metabolism was examined by 31P NMR Spectroscopy at 14th day. Tumor size was measured at 21 st day..The results of 31P NMR Spectroscopy were compared with the size of tumor. Pcreatine and beta ATP had a better correlation with the tumor size.

(2 results)