Basic Study of Serial Magnetic Resonance Imaging in Small Animal Model of Cerebral Ischemia

1992 Fiscal Year Final Research Report Summary

• Project/Area Number: 02670621
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: UNIVERSITY OF TSUKUBA
• Principal Investigator: HYODO Akio Univ. of Tsukuba, Inst. Clin. Med. Sci., Assist. Prof., 臨床医学系, 講師 (40167606)
• Co-Investigator(Kenkyū-buntansha): NOSE Tadao Univ. of Tsukuba, Inst. Clin. Med. Sci., Prof., 臨床医学系, 教授 (10009699) ; YOSHIZAWA Takashi Univ. of Tsukuba, Inst. Clin. Med. Sci., Assist. Prof., 臨床医学系, 講師 (00210667) ; MATSUMURA Akira Univ. of Tsukuba, Inst. Clin. Med. Sci., Assist. Prof., 臨床医学系, 講師 (90241819)
• Project Period (FY): 1990 – 1992
• Keywords: Magnetic Resonance / Cerebral Ischemia / Experimental Model / Rat / Rabbit / MR Spectroscopy / Gd-^<14>C-DTPA / Pathohistology

Research Abstract

The serial magnetic resonance imaging (MRI) in small animal model of cerebral ischemia was studied and correlated with tetorazolium staining and histopathological findings. In rabbit model of cerebral ischemia, the ischemic lesion was detected by MRI in T_2-weighted image or gadolinium-enhanced T_1- weighted image as high intensity area from early stage after ischemic episode.
The MRI finding was correlated very well with tetorazolium staining and histopathological findings. The ischemic area detected by MRI was almost same as that detected by tetorazolium staining and histopathological finding. However, in ultra-early stage after ischemic episode, MRI might underestimate the area of cerebral ischemia.
The effect of a paramagnetic contrast agent, gadolinium(Gd)-DTPA, on MRI of cerebral ischemia was studied basically in a rat model of focal cerebral ischemia using Gd-^<14>C-DTPA autoradiograph. As a result,the accumulation of the radioactivity of Gd-^<14>C-DTPA was detected in the area of cerebral ischemia, so Gd-DTPA may be useful for the enhancement of the detect of the ischemic lesion.
Magnetic resonance spectroscopy(MRS) in experimental cerebral ischemia was also studied in a rat model of diffuse cerebral ischemia.^<31>P -MRS was detected 20 minutes after cerebral ischemia, and increasing of inorganic phosphate and decreasing of ATP were recognized as a disturbance of cerebral metabolism after cerebral ischemia. However it is still difficult to detect the serial findings of^<31>P -MRS continuously and we hope we will study it in near future.