1992 Fiscal Year Final Research Report Summary
The effect of interleukin 6 on hypothalamo pituitary axis
| Project/Area Number |
02670738
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
HIROTA Kenji Osaka University Medical School/Lecture, 医学部, 講師 (00189888)
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| Co-Investigator(Kenkyū-buntansha) |
KOIKE Koji Osaka Univ. Med. School Research Assistant, 医学部, 助手 (70225340)
IKEGAMI Hiromasa Osaka Univ. Med. School Research Assistant, 医学部, 助手 (10184409)
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| Project Period (FY) |
1990 – 1992
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| Keywords | interleukin-6 / receptor / estrogen / cell growth / paracrine |
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| Research Abstract |
There is accumulating evidence of a close linkage between neuroendocrine and immune system. Study is designed to investigate the effect of interleukin 6 (IL-6) on hypothalamo -pituitary-ovarian axis. First, we demonstrated that IL-6 was produced in dispersed hypothalamic cells, and estrogen and tumor necrosis factor-alpha (TNF-alpha) stimulated IL-6 production. Further we have shown that IL-6 stimulated Gn-RH release from hypothalamic cells, suggesting that cytokines such as IL-6 and TNF-alpha is involved in the feedback mechanism of estrus cycle. We also demonstrated IL-6 production, stimulation of IL-6 production by interferon and TNF-alpha and the persence of IL-6 binding site in the rat pituitary cells. In our preliminary experiment using folliculo-stellate (FS) cell line, we also observed that FS cells produced IL-6, which is stimulated by VIP and PACAP, and that IL-6 stimulated cell growth of rat pituitary clonal cell lines. Therefore, it is possible that IL-6 produced by FS cells might participate in the local regulation of cell growth, in addition to modulation of the secretory process of the hormone-secreting pituitary cells.
In conclusion, IL-6 is produced by FS cells and regulates hormone production and cell growth in the pituitary gland via a paracrine mechanism. Our study also opens new perspectives on the physiological role of FS cells in relation to the biological action of IL-6.
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[Publications] Koike, K., Miyake, A., Aono, T., Sakumoto, T., Ohmichi, M., Yamaguchi, M. and Tanizawa, O: "Effect of prolactin on the secretion of hypothalamic GnRH and pituitary gonadotropins" Hormone Res. 35. 5-12 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Koike, K., Masumoto, N., Kasahara, K., Yamaguchi, M., Tasaka, K., Hirota, K., Miyake, A. and Tanizawa, O: "Tumor necrosis factor-alpha stimulates prolactin release from anterior pituitary cells: A possible involvement of intracellular calcium mobilization" Endocrinology. 128. 2785-2790 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Koike, K., Hirota, K., Ohmichi, M., Kadawaki, K., Ikegami, H., Yamaguchi, M., Miyake, A. and Tanizawa, O: "Tumor necrosis factor-alpha increases release of arachidonate and prolactin from anterior pituitary cells" Endocrinology. 128. 2791-2798 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamaguchi, M., Koike, K., Yoshimoto, Y., Matsuzaki, N., Miyake, A. and Tanizawa, O: "Interleukin-6 stimulates gonadotropin-releasing hormone secretion from rat hypothalamic cells" Horm Res. 35. 252-256 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Ohmichi, M., Hirota, K., Koike, K., Kurachi, K., Ohtsuka, S., Matsuzaki, N., Yamaguchi, M., Miyake, A. and Tanizawa, O: "Binding sites for interleukin-6 in the anterior pituitary gland" Neuroendocrinology. 55. 199-203 (1992)
Description
「研究成果報告書概要(欧文)」より
