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1993 Fiscal Year Final Research Report Summary

Molecular- and Cell-Biological Study of Plant Senescence

Research Project

Project/Area Number 03304005
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionThe University of Tokyo (1993)
Nagoya University (1991-1992)

Principal Investigator

WATANABE Akira  The University of Tokyo ; Graduate School of Science ; Professor, 大学院理学系研究科, 教授 (70023471)

Co-Investigator(Kenkyū-buntansha) MAE Tadahiko  Tohoku University ; Faculty of Agriculture ; Assosiate Professor, 農学部, 助教授 (60134029)
KATOH Sakae  Toho University ; Faculty of Science ; Professor, 理学部, 教授 (50011515)
IMASEKI Hidemasa  Nagoya University ; School of Agricultual Science ; Professor, 農学部, 教授 (90023431)
OHSUMI Yoshinori  The University of Tokyo ; College of Arts and Science ; Assosiate Professor, 教養学部, 助教授 (30114416)
ISHI Ryuichi  The University of Tokyo ; Faculty of Agriculture ; professor, 農学部, 教授 (00011958)
Project Period (FY) 1991 – 1993
Keywordssenescence / senescence in plants / photosynthesis / chloroplast / translocation of nitrogen / cDNA cloning / gene expression / ethylene
Research Abstract

Ishii observed that diffusion resistance of CO^2 increases to result in inefficient photosynthesis in senesing leaves. Katoh deduced from various measurements of photosynthesis that a part of decrease in photosynthesis in shadowed leaves can be regarded as a kind of adaptation of photochemical reaction system to the lower light intensities. As RuBisCO protein was known to markedly decrease in senescent leaves, Mae was successful in detecting the degradation products of its large subunit in chloroplasts from senecing rice leaves.
Fukuda was successful in isolating cDNA clones for a protease specific to developing tracheary elments, in which cellular constituents are degraded similarly to senescing cells. Watanabe found that two of the three members of cytosolic glutamine synthetase genes are activated in senescing radish regulated. Yamaya found that this glutamine synthetase localizes only in vascular bundle cells in rice. He also found that NADH-dependent glutamate synthase localizes specifically in very young unexpanded leaves and developing grains, and is, therefore responsible for receiving glutamine that is translocated from source organs.
Kawasaki had a progress in preparing RFLP makers to isolate a gene which is responsible for a stay-green mutation in rice. Azumi analyzed the function of promoter region of his dark-inducible gene from radish in transgenic tobacco plants.

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Suzuki,K.Kasamo,K.: "Effects of aging on the ATP-and pyrophosphate-dependent pumping of protons across the tonoplast isolated from pumpkin cotyledons" Plant Cell Physiol. 34. 613-619 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishimura,Mikio: "Leaf peroxisomes are directly transformed to glyoxysomes during senescence of pumpkin cotyledons." Protoplasma. 175. 131-137 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 林誠: "ミクロボディ機能変換の分子機構" 植物細胞工学. 5. 357-365 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ishitani,T.Takabe,K.Kojima,T.Takabe: "Regulation of Glucinebetaine Accumulation in the Halotorerant Cyanobacterium Aphanothece halophytica" Aust.J.Plant Physiol.20:(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Satoh,H.Mori and H.Imaseki: "Monomeric and dimeric forms of and mechanism-based inactivation of 1-aminocyclopropane-1-carboxylate synthase." Plant Cell Physiol.34. 753-760 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ofosu-Anim,J.Yamaki,S: "Sugar content,compartmentation and efflux in strawberry fruit tissue" J.Amer.Soc.Hort.Sci.119(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaki,S.Ito,M.Ofosu-Anim,J Ozaki,S.Tshuchimoto,Y: "Cellular compartmentation and transport into tonoplasts resicles of sugars with ripening of pear fruit" Acta Horticulturae. 343. 12-17 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaki,S.Ino,M: "Alteration of cellular compartmentation and membrane permeability to sugars in immature and mature apple fruit" J.Amer.Soc.Hort.Sci. 117. 951-954 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川崎信二: "イネの老化変異体の性質と植物の膜タンパク質の機能解析" 第31回ガンマーフィールド・シンポジウム/作物の新機能開発と突然変異の利用. 10-13 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Junichi Ueda,Kensuke Miyamoto and Seiichiro Kamisaka: "Separation of a new type of plant grwoth regulator,jasmonates,by chromatographic procedures" Journal of Chromatography. (in press). (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川崎信二: "葉の老化のしくみを探る" 化学と生物. 28. 560-562 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川崎信二: "イネの2種類の老化異常変異体の性質について" 日本植物生理学会1190年度年会講演要旨集. 270 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayakawa,T.,Yamaya,T.,Mae,T.Ojima,K.: "Changes in the content of two glutamate synthase proteins in spikelets of rice(Oryza sativa) plants during ripening." Plant Physiology. 101. 1257-1262 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayakawa,T.,Nakamura,T.,Hattori,F.,Mae.T.,Ojima.K.,Yamaya.T.: "Cellular localization of NADH-deplndent glrtrmate-synthase protein in vascular bandles of unexpanded leaf blades and young grains of rice plants." Planta. 193(印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda,H.,Yoshimura,T.,Sato,Y.and Demura,T.: "Molecular mechanism of xylem differentiation" J.Plant Research Special Issue. 3. 97-107 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato,Y.,Sugiyama,M.Gorecki,R.J.,Fukuda,H.and Komamine,A.: "Interrelationship between lignin deposition and the activities of peroxidase isozymes in differentiating tracheary elements of Zinnia:Analysis with L-a-aminooxy-b-phenylpropionic acid and 2-aminoindan-2-phosphonic acid" Planta. 189. 584-589 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Demura,T.and Fukuda,H.: "Molecular cloning and characterization of cDNAs associated with tracheary element differentiation in cultured Zinnia cells" Plant Physiology. 103. 815-821 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda,H.: "Redifferentiation of single mesophyll cells into tracheary elements" International J.Plant Science. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konishi,T.Watanabe,A.: "Transport of proteins into the thylakoid lumen-Stromal processing and energy requirements for the import of the precursor tD the 23-kDa protein of PS II" Plant Cell Physiol. 34. 315-319 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konishi,T.Maruta,Y.Shinohara,K.Watanabe,A.: "Transit peptides of thylakoid luminal proteins:the sites of stromal processing are conserved among higher plants" Plant Cell Physiol. 34. 1081-1087 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawakami,N.Watanabe,A.: "Translatable mRNA for chloroplast-targeted proteins of detached radish cotyledons senescing in the dark" Plant Cell Physiol. 34. 697-704 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,A.: "植物はなぜ枯れるのか-その仕組みと意義" 日本の科学と技術. 34. 32-37 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤栄: "Acclimation and senescence of leaves:Their role in canopy Photosynthesis." Photosynthetic Responses to the Environment.H.Y.Yamamoto & C.M.Smith,eds.1-13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 加藤栄: "Effects of light,nutrient and age on distribution of nitrogen in leaves of a vine grown horizontally to avoid mutual shading." Oecologia. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 笠毛邦弘(共著): "基礎生物学講座5 植物の生理 6章 発芽から老化まで-植物の成長-" 朝倉書店, 39 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木健策,笠毛邦弘: "研究ジャーナル 老化と生体膜" 農林水産技術情報協会, 7 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe, A.: "Transport of proteins into the thylakoid lumen - Stromal processing and enegy tequirements for the import of the precursor to the 23-kDa protein of PSII" Plant Cell Physiol.34. 315-319 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, A.: "Transit peptides of thylakoid luminal protein : the sites of stromal processing are conserved among higher plants." Plant Cell Physiol.34. 1081-1087 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, A.: "Translatable mRNA for chloroplast-targeted protein of detached radish cotyledons senescing in the dark." Plant cell Physiol.34. 697-704 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh, S.: "Effects of light, nutrient and age on distribution of nitorogen in leaves of a vine grwon horizontally to avoid mutual shading." Oecologia. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kasamo, K.: "Effect of aging on the ATP- and pyrophosphate-dependent pumping of protons across the tonoplast isolated from pumpkin cotyledons." Plant Cell Physiol.34. 613-619 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishimura, M.: "Leaf peroxisomesare direcly transformed to glyoxysomes during senescence of pumpkin cotyledons." Protoplasma. 175. 131-137 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takabe, T.: "Regulation of glycinebetaine accumulation in the halotorerant cyanobacterium Aphanothece halophytica." Aust. J.Plant Physiol.20. (in press) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imaseki, H.: "Monomeric and dimeric forms of and mechanism-based inactivation of 1-aminocyclopropane-1-carboxylate synthase." Plant Cell Physiol.34. 753-760 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaki, S.: "Sugar content, compartmentation and efflux in strawberry fruit tissue." J.Amer. Soc. Hort. Sci.119. (in press) (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaki, S.: "Cellular compartmentation and transport into tonoplast vesicles of sugar with ripening of pear fruit." Acta Horticult.343. 12-17 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaki, S.: "Alteration of cellular compartmentation and membrane permeability to sugars in immature and mature apple fruit." J.Amer. Soc. Hort. Sci.117. 951-954 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae, T.: "Leaf development in Lolium temulentum : Photosynthesis and photosynthetic proteins in leaves senescing under different irradiances." Plant Cell Physiol.34. 391-399 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae, T.: "Changes in the content of two glutamate synthase proteins in spikelets of rice (Oryza sativa) plants during ripening." Plant Physiol.101. 1257-1262 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaya, T.: "Cellular localization of NADH-dependent glutamate-synthase protein in vascular bundle of unexpanded leaf blades and young grain of rice." Planta. 193 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ueda, J.: "Separation of a new type of plant growth regulator, jasmonates, by chromatografhic procedures." J.Chromatogr.(in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, H.: "Molecular mechanism of xylem defferentiation." J.Plant Res. Special Issue. 3. 97-107 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, H.: "Interrelationship between lignin deposition and the activities of peroxidase isozymes in differetiating tracheary elements of Zinnia : Analysis with L-alpha-aminooxy-beta-phenylpropionic acid and 2-aminoindan-2-phosphonic acid." Planta. 189. 584-589 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, H.: "Molecular cloning and charactarization of cDNAs associated with tracheary element differentiation in cultured Zinnia cells." Plant Physiol.103. 815-821 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, H.: "Redifferentiation of single mesophyll cells into tracheary elements." Int. J.Plant Sci.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawasaki, S.: "Analysis of leaf senescence through mutation and affinity-labeling." Gamma Field Symposia.31. 45-65 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katoh, S.: "Acclimation and senescence of leaves : Their role in canopy photosynthesis." Photosynthetic Responses to the Environment, Yamamoto and Smith eds.1-13 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mae, T.: "Leaf constituents and photosynthesis." Science of the Rice Plants, Matsuo et al. eds.2 : (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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