1993 Fiscal Year Final Research Report Summary
Stress response and stress protein
| Project/Area Number |
03304051
|
|---|
| Research Category |
Grant-in-Aid for Co-operative Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
物質生物化学
|
|---|
| Research Institution | The Tokyo Metropolitan Institute of Medical Science |
|---|
Principal Investigator |
YAHARA Ichiro Tokyo Met.Inst.Med.Sci., Vice-Dir., 副所長 (60109957)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKASAWA Toshio Keio Univ., Fac.Med., Prof., 医学部, 教授 (90029934)
KAGAWA Yasuo Jichi Med.Sch., Fac.Med., Prof., 医学部, 教授 (30048962)
NAGATA Kazuhiro Kyoto Univ., Inst.Chest Dis., Prof., 胸部疾患研究所, 教授 (50127114)
AIBA Hiroji Nagoya Univ., Fac.Sci., Prof., 理学部, 教授 (20025662)
ISHIHAMA Akira Nat.Inst.Genet., Mol.Genet., Prof., 分子遺伝, 教授 (80019869)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Keywords | stress response / stress proteins / transcriptional control / molecular chaperone / domain structure / temperature-sensitive mutants / environmental adaptation / cell defense mechanism |
|---|
| Research Abstract |
The synthesis of heat shock proteins in Escherichia coli was shown to be regulated by increasing amounts of the transcription factor sigma^<32>. Functions of E.coli stress proteins involved in DNA replication and secretion have been analyzed using temperature-sensitive mutants. Evidence has been presented that E.coli adapts environmental atresses by modifying both transcriptional and translational machinaries. We also found that heat shock induces relaxation of super-coiled plasmid DNA in E.coli. Stress proteins of thermophilic bacterium were investigated. Stress response of the budding yeast, Saccharomyces cereviseae, was analyzed using various mutants. Domain atructure, stress-induced alteration in biophysical properties, and tissuespecific expression of HSP90 were investigated. Human HSP40 was identified by cDNA cloning to be a homologue of E.coli DnaJ.Function and expression of the collagen-specific chaperone, HSP47, were extensively analyzed.
|
