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1993 Fiscal Year Final Research Report Summary

STUDY ON ACTION MECHANISM OF BRASSINOSTEROIDS

Research Project

Project/Area Number 03305009
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionTEIKYO UNIVERSITY

Principal Investigator

YOKOTA Takao  TEIKYO UNIV, DEPT OF BIOSCI, PROFESSOR, 理工学部, 教授 (40011986)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Yasutomo  UTSUNOMIYA UNIV, WEED SCI RES CENTER, PROFESSOR, 雑草科学センター, 教授 (90008003)
TAKATSUTO Suguru  JOETSU UNIV OF EDUC, DEPT OF CHEM EDUC, ASSOCIATE PROFESSOR, 学校教育学部, 助教授 (30179460)
ABE Hiroshi  TOKYO UNIV OF AGRIC AND TECH, DEPT OF APPLIED BIOL SCI, PROFESSOR, 応用生物科学科, 教授 (10015084)
SAKURAI Akira  INST OF PHYS CHEM RES (RIKEN), CHIEF RESEARCHER, 主任研究員 (10087562)
KATSUMI Masayuki  ICU, DEPT OF EDUCATION, PROFESSOR, 教養学部, 教授 (10052242)
Project Period (FY) 1991 – 1993
KeywordsBRASSINOSTEROID / BIOSYNTHESIS / GROWTH / MICROTUBLE / BLUE LIGHT / ANTIBRASSINOSTEROID / CONJUGATE / MEMBRANE STABILITY
Research Abstract

1)A biosynthetic pathway of brassinolide (teasterone -> typhasterol -> cstasterone -> brassinolide) is found in both cell cultures and intact plant tissue. 3-Dehydrotesterone was found as an intermediate between teasterone and typhasterol
2)In pollen of Lolium perenne, brassinosteroid is distributed in amyloplast as examined by immunocytochemistry.
3)A myristate ester of teasterone was identified from lily pollen.
4)Biological activity of brassinosteroids in rice leaf was increased by irradiation of blue light which causes unrolling of the rice leaf. However, this is not due to increased level of endogenous brassinosteroids.
An antibrassinolide termed KM-01 was isolated from a fungus Drechslera avenae and the chemical structure was determined.
Slow-releasing synthetic brassinolides were synthesized and a candidate was seltected for practical agricultural use.
7)Conditioning effects of brassinolide on seed germination of Striga and Orobanche were analyzed.
8)In contrast to auxin, growth effect of brassinosteroids is related with microtuble behavior and with inner layr of stem tissue.
9)Cold tolerance effects of brassinosteroids are based on their stabilizing effects of plant membrane.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] T.Iwasaki: "Brassinosteroids act as regulators of tracheary element differentiation in isolated Zinnia mesophyll cells" Plant & Cell Physiology. 32. 1007-1014 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Suzuki: "Metabolism of castasterone and brassinolide in mung bean explant" Phytochemistry. 33. 1361-1367 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Suzuki: "Biosynthesis of brassinosteroids in Catharanthus roseus.Part I Biosynthesis of brassinolide from castasterone in cultured cells of Catharanthus roseus" J.Plant Growth Regul.12. 101-106 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Abe: "Effect of Introducing a lacfone group into typhasterol and teasterone to promote rice eamira inclination" Biosci.Biotech.Biochem.56. 1312-1313 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Tominaga: "Brassinolide-induced elongation of inner tissues of squash(Cucurbit a maxima Duch.)hypocotyl segments" Plant & Cell Physiology. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.-K.Kim: "Brassinolide-Inhibitor KM-01,its isolation and structure elucidation from a fungus Drechslera avenae" Tetrahedron Letters. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.G.Cutler: "Brassinosteroids.Chemistry,Bioactivity & Applications" アメリカ化学会, 358 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T Yokota: "Transport and metabolism of brassinosteroids in rice" Progress in Plant Growth Regulation (ed. CM Karssen et al), Kluwer Academic Publishers. 298-305 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Suzuki: "Metabolism of castasterone and brassinolide in mung bean explant" Phytochemistry. 33. 1361-1367 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Suzuki: "Biosynthesis of brassinolide from castasterone in cultured cells of Catharanthus roseus" J Plant Growth Regul. 12. 101-106 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Suzuki: "Biosynthesis of brassinolide from teasterone via typhasterol and castasterone in cultured cells of Catharanthus roseus" J Plant Growth Regul. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Abe: "Rice lamina inclination, endogenous levels in plant tissues and accumulation during pollen development of brassinosteroids" Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 200-207 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Asakawa: "Teasterone 3-myristate : A new type of brassinosteroid derivative in Lilium longiflorum anthers." Biosci Biotech Biochem. 58. 219-220 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H Abe: "Effect of blue light on endogenous brassinosteroid activity and leaf unrolling in rice seedlings" Plant Physiol. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S-K Kim: "A brassinolide-inhibitor KM-01, Its isolation and structure elucidation from a fungaus Drechslera avenae." Tetrahed Lett.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Kamuro: "Practical uses of brassinolide derivatives : Promoting effects on feritilization and seed setting" Proceedings of 19th Annual Meeting of Plant Growth Regulator Society of America (San Fransisco, July 17-10). 275-277 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Takeuchi: "Effects of brassinolide on conditioning and germination of witchweed (Striga asiatica) seeds." Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 298-305 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y Takeuchi: "Effects of brassinosteroids on conditioning and germination of clover broomrape (Orobanche minor)seeds." J Plant Growth Regul. (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S Kuraishi: "Effect of brassinolide on levels of indoleacetic acid and abscisic acid in squash hypocotyls" Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 312-319 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R Tominaga: "Brassinolide-induced elongation of inner tissues of squash (Cucurbita maxima duch.) hypocotyl segments." Plant Cell Physiol. (submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T Iwasaki: "Brassinosteroids act as regulators of tracheary-elements differentiation in isolated Zinnia mesophyll cells." Plant Cell Physiol. 32. 1007-1014 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M Katsumi: "Brassinolide protects plant tissues from chilling injury" (to be submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M Seki: "Effect of brassinolide on cortical microtuble arrangements under chilling stress." Abstracts of papers, XI International Botanical Congress (Yokohama, Aug 28-Sep 3). 457 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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