1993 Fiscal Year Final Research Report Summary
Studies on the control of signaling molecules expressed in hairpin-loop linear DNA plasmid in the plant pathogenic fungus
| Project/Area Number |
03454052
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物保護
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
HASHIDA Teruyoshi Tohoku University, Faculty of Agriculture, Assistant Professor, 農学部, 助教授 (20189476)
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| Co-Investigator(Kenkyū-buntansha) |
KARASAWA Akira Tohoku University, Faculty of Agriculture, Tutor, 農学部, 助手 (90204651)
TAKAHASHI Hideki Tohoku University, Faculty of Agriculture, Tutor, 農学部, 助手 (20197164)
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| Project Period (FY) |
1991 – 1993
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| Keywords | Specific protein / ORF of pRS64 / Plasmid-derived transcript / Protein of 28kDa / Amino acid Sequence / Plasmid-encoded protein / Sequence homology between pRS64 and RNA |
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| Research Abstract |
The plant pathogenic isolate RI-64 of anastomosis group 4 of Rhizoctonia solani possesses three linear DNA plasmids (pRS64-1, -2, and -3). Unique poly(A)^-RNA of 0.5 kb hybridizable with the pRS64 DNAs was found in mycelial cells of the isolate RI-64. The overall homology at the nucleotide level between pRS64-1, -2, and -3 and the cDNA prepared for the poly(A)^-RNA was 100%, 73%, and 84%, respectively. The open reading frames found in pRS64-1, -2, and -3 (ORF1-1, ORF2-1, and ORF3-1) were 68 amino acids long. The amino acid sequence showed no significant homology with known properties. Extracts from E.coli cells expressing ORF1-1 gave a specific protein of 7 kDa. Antisera raised to the ORF1-1 product obtained from E.Coli cells cross reacted with the specific proteins found in the mycelia. The results indicate that the DNA plasmids found in the mycella. The results indicate that the DNA plasmids found in R.solani contain a sequence for a specific protein, that may be involved in determination of pathogenicity to plants.
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