New procedure for early detection of rejection on transplanted lung

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 03454333
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: KYOTO UNIVERSITY
• Principal Investigator: HITOMI Sigeki Kyoto University, Chest Disease Research Institute, Professor, 胸部疾患研究所, 教授 (80173186)
• Co-Investigator(Kenkyū-buntansha): YOKOMISE Hioroyasu Kyoto University, Chest Disease Research Institute, Assistant Professor, 胸部疾患研究所, 助手 (80231728) ; WADA Hiromi Kyoto University, Chest Disease Research Institute, Associate Professor, 胸部疾患研究所, 助教授 (90167205)
• Project Period (FY): 1991 – 1993
• Keywords: lung transplantation / rejection / Adult T cell leukemia derived factor

Research Abstract

Recently lung transplantation is an accepted therapeutic option for patients with end-stage lung disease. However, an early detection of rejection is major obstruction for success of lung transplantation. Adult T cell leukemia derived factor (ADF) has been shown to be induced by a viriety of streses. In this study we exmined ADF expression in bronchoalveolar lavage (BAL) cells after canine lung transplantation in order to determine whether it could be used for early detection of rejection. Allotransplantations were performed in adult mongrel dogs, and FK506(0.1mg/kg : im) was administerd from the day of the operation to the 5th post operative day (POD). No immunosuppression was given from the 6th POD to 10th POD.Animals were sacrificed on the 10th POD.BAl was performed on the 5th and 10th POD.BAL cells and transplanted lungs were analyzed using anti-ADF anti-body. ADF in BAL cells was significantly (p<0.01) presented in the 10th POD than the 5th POD.In infected lung, ADF was not induced compared to rejected lungs. In summary, the analysis of BAL cells for ADF may be useful in the early diagnosis of rejection.