Heart preservation and immunological tolerance induction in orthotopic heart transplantation in swines.

1992 Fiscal Year Final Research Report Summary

• Project/Area Number: 03454335
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: Thoracic surgery
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: YASUI Hisataka Kyusyu University, Faculty of Medicine, Associate professor, 医学部, 助教授 (20089923)
• Co-Investigator(Kenkyū-buntansha): MASUDA Munetaka Kyusyu University, Faculty of Medicine, Assistant professor, 医学部, 助手 (10190365) ; KOHNO Hiroyuki Kyusyu University, Faculty of Medicine, Assistant professor, 医学部, 助手 (80178227) ; TOMINAGA Ryuji Kyusyu University, Faculty of Medicine, Lecturer, 医学部, 講師 (70136464) ; KAWACHI Yoshito Kyusyu University, Faculty of Medicine, Lecturer, 医学部, 講師 (80117093)
• Project Period (FY): 1991 – 1992
• Keywords: Cyclophosphamide / Tolerance / Skin graft / Orthotopic heart transplantation

Research Abstract

Cyclophosphamide-induced immunological tolerance was further studied in various fully allogeneic murine combination, while a system of orthotopic heart transplantation was tried to established in swines by using cardiopulmonary bypass.
Exp.1. When a recipient mouse was given 100mug anti Thy 1.2mAb i.v. on day-1,9x10^7 fully allogeneic spleen cells plus 3x10^7 bone narrow cells i.v. on day 0, and 200mg/kg cyclophosphamide on day 2, a long-lasting skin graft tolerance to the tolerogenic strain was induced in various fully allogeneic murine combinations such as C3H(H-2^k)*B6(H-2^b), B6*C3H, BALB(H-2^d)*B6, and BALB*C3H. The median survival (days) was 123 (n=19), 136(n=9), >503(n=15), and >165(n=8), respectively.
Exp.2. Female swines (20-25kg) were used. Orthotopic heart transplantation was per-formed using cardiopulmonary bypass. The donor heart was arrested with Kyushu University cardioplegic solution (CP;Na87,K20)and then flushed with and emerged in the CP or in Collins' solution (C;Na10,K117). The preservation was performed at 0ﾟC for 4 hours. One hour after the aortic declamping, Emax was examined. Each group contained 5 pigs. Cardiopulmonary bypass was able to discontinue in 3(60%) in the CP group and in 1(20%) in the C group. Only one of the 3 of the CP group could be examined for Emax. LOS and bleeding was the major complications. Any long-term survivors were not obtained.