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1993 Fiscal Year Final Research Report Summary

Genetical and biochemical characterizations of hemorrhagic fever with renal syndrome virus infection in mice.

Research Project

Project/Area Number 03558009
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionHokkaido University

Principal Investigator

ARIKAWA Jiro  Institute of Immunological Associate Science, Hokkaido University Professor, 免疫科学研究所, 助教授 (10142704)

Co-Investigator(Kenkyū-buntansha) ISEGAWA Yuji  Research Institute for Assistant Microbial Diseases, Osaka Professor University, 微生物病研究所, 助手 (20184583)
ISHIHARA Chiaki  School of Veterinary Professor Medicine, Rakuno Gakuen University, 獣医学科, 教授 (90082172)
YOSHIMATSU Kumiko  Institute of Immunological Research Science, Hokkaido University Assistant, 免疫科学研究所, 教務職員 (90220722)
ITAKURA Chitoshi  Faculty of Veterinary Professor Medicine, Hokkaido University, 獣医学部, 教授 (30021695)
HASHITOMO Nobuo  Faculty of Veterinary Professor Medicine, Hokkaido University, 獣医学部, 教授 (60082103)
Project Period (FY) 1991 – 1993
KeywordsHemorrhagic fever with renal syndrome / Hantavirus / Animal model / Scid mouse / PCR / Virus infection / Animal experiment / Clinical biochemistry
Research Abstract

1. SCID mice inoculated i.p. with Hantaan virus and Seoul virus developed systemic infection and died after 32 to 38 days post infection.
2. Viral antigen was detected from various organs including kidney. Although no hemorrhagic manifestations was seen in kidney, the SCID mice showed increased level of BUN after infection.
3. RT-PCR followed by Nested-PCR method was established for detecting Hantaan virus genome in various organs in infected mice.
4. SCID mice (21 days post infection) were passively administered with spleen cells which were prepared from normal BALB/c mice. Seven days after the cell transfer, SCID mice began to produce serum antibody to Hantaan virus. In accordance with the appearance of antibody, BUN levels transiently increased. These results suggest the immune response mediated pathology of Hantavirus infection.
These results suggest the possible application of SCID mice as the model for hemorrhagic fever with renal syndrome in human. However, the infected SCID mice did not show any hemorrhagic manifestation nor pathologic changes. Further characterizations with the transfer of immune spleen cells and measurement of cytokines in serum will be required.

  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Yao,J.-S.: "Antibody-dependent enhancement of hantavirus ifection in macrophge cell lines." Archives of Virology. 122. 107-118 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi,K.: "Renal lesions in rats infected with Rattus serotype hantavirus (SR-11 strain)." Journal of Veterinary Medicine. B38. 657-664 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mori,F.: "Pathological studies on central nervous tissues of rats infected with Rattus serotype hantaviruses." Journal of Veterinary Medicine. B38. 665-672 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamura,K.: "Susceptibility of laboratory and wild rodents to Rattus or Apodemus-type hantaviruses." Acta Virologica. 35. 54-63 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 苅和宏明: "ドブネズミにおける腎症候性出血熱ウイルス感染の疫学的研究" 北海道獣医師会誌. 35. 69-74 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arikawa,J.: "Protective role of antigeni sites on the envelope protein of Hantaan virus defined by monoclonal antibodies." Archives of Virology. 126. 271-281 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa,H.: "Development and application of protein G antibody assay for the detection of antibody to hantavirus." Journal of Virological Methods. 37. 345-354 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yao,J.-S.: "Effect of neutralizing monoclonal antibodies on Hantaan virus infection of the macrophage P388D1 cell line." Japanese Journal of Veterinary Research. 40. 87-97 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Azuma,I.: "Stimulation of host-defense mechanism with synthetic adjuvants and recombinant cytokines against viral infection in mice." Microbial Infections. 19. 253-263 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Isegawa,Y.: "Selective amplification of cDNA sequence from total RNA by cassette-ligation mediated polymerase chain reaction(PCR):Application to sequencing 6.5 kb genome segment of hantavirus strain B-1." Molecular and Cellular Probes. 6. 467-475 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arikawa,J.: "Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies." Archives of Virology. 126. 271-281 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大島淳: "PCR法による腎症候性出血熱ウイルスHantaan株の微量検出法" 日本臨床. 50. 305-309 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishihara,C.: "Anti-viral activity of sulfated chitin derivatives against Friend Leukemia and herpes simplex type-1 viruses." Vaccine. 11. 670-673 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimatsu,K.: "Protective immunity of Hantaan virus nucleocapsid protein studied using the baculovirus-expressed proteins." Archives of Virology. 130. 365-376 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoo,Y.-C.: "Comparison of virulence between Seoul type virus strain SR-11 and Hantaan type virus strain 76-118 of hantaviruses in suckling mice." Microbiology and Immunology. 37. 557-562 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshimatsu,K.: "Application of recombinant baculovirus expressed hantavirus nucleocapsid protein as a diagnostic antigen inIFA test" Journal of Veterinary Medical Science. 55. 1047-1050 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有川二郎: "特集腎症候性出血熱(HFRS);最近の話題,免疫学的・診断学的立場から." アニテックス. 5. 277-281 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 伊勢川裕二: "特集腎症候性出血熱(HFRS);最近の話題,腎症候性出血熱ウイルス." アニテックス. 5. 273-276 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arikawa,J.: "Epizootiological studies of hantavirus infection among urban rats in Hokkaido,Japan" Journal of Veterinary Medical Science. 56. 27-32 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa,H.: "Enhancement of infectivity of hantavirus in cell culture by centrifugation." Journal of Virological Method. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kariwa,H.: "Comparison of nucleotide sequences of M genome segments among Seoul virus strains isolated from Eastern Asia." Virus Research. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, K., Itakura, C., Arikawa, J.and Hashimoto, N.: "Renal lesions in rats infect-ed with Rattus serotype hantavirus(SR-11 strain)." Journal of Veterinary Medicine. B38. 657-664 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mori, F., Kobayashi, K., Itakura, C., Arikawa, J., Hashimoto, N.: "Pathologic studies on central nervous tissues of rats infected with Rattus serotype hantavirus(SR-11 strain)." Journal of Veterinary Medicine. B38. 665-672 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamura, K., Zhang, X.-K., Arikawa, J., Takashima, I., Dempo, K.and Hashimoto, N.: "Susceptibility of laboratory and wild rodents to Rattus or Apodemus-type Hantaviruses." Acta virologica. 35. 54-63 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa, H., Arikawa, J., Yao, K.-S., Takashima, I., Hashimoto, N.: "Epidemiological studies on wild rats" Hokkaido Jyuishikai Zasshi (in Japanese). 35(3). 69-74 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arikawa, J., Yao, J.-S., Yoshimatsu, K., Takashima, I.and Hashimoto, N.: "Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies." Archives of Virology. 126. 271-281 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Azuma, I., Ishihara, C., Iida, J., Yoo, Y.C., Yoshimatsu, K.and Arikawa, J.: "Stimu- lation of host-defense mechanism with synthetic adjuvants and recombinant cytokines against viral infection in mice." Microbial Infections Friedman (eds). 253-26.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa, H., Arikawa, J., Takashima, I.and Hashimoto, N.: "Development and application of protein G antibody assy for the detection of antibody to hantavirus." Journal of Virological Methods. 37. 345-354 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yao, J.-S., Kariwa, H., Takashima, I., K., Yoshimatsu, Akikawa, J.and Hashimoto, N.: "Antibody dependent enhancement of Hantavirus infection in macrophage cell lines." Archives of Virology. 122. 107-118 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yao, J.-S., Akikawa, J., Kariwa, H., Yoshimatsu, K., Takashima, I.and Hashimoto, N.: "Effect of neutralizing monoclonal antibodies on Hantaan virus intection of the macrophage P388D1 cell line." Japanese Journal of Veterinary Research. 40. 87-97 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arikawa, J., Yao, J.-S., Yoshimatsu, K., Takashima, I., Hashimoto, N.: "Protective role of antigenic sites on the envelope protein of Hantaan virus defined by monoclonal antibodies." Archives of Virology. 126. 271-281 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohshima, A., Isegawa, Y.: "Detection of HFRS Hantaan virus RNA sequence by PCR." Nippon Rinsho (in Japanese). 50. 305-309 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isegawa, Y., Sheng, J., Sokawa, Y., Yamanishi, K., Nakagomi, O., Ueda, S.: "Selective amplification mediated polymerase chain reaction (PCR) : Application to sequencing 6.5Kb genome segment of hantavirus strain B-1." Molecular and Cellular Probes. 6. 467-47

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishihara, C., Yoshimatsu, K., Tsuji, M., Arikawa, J., Saiki, I.and Azuma, I.: "Anti- viral activity of sulfated chitin derivatives against Friend Leukemia and herpes simplex type-1 viruses." Vaccine. 11. 670-673 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimatsu, K., Yoo, Y.-C., Yoshida, R., Ishihara, C., Azuma, I.and Arikawa, J.: "Protective immunity of Hantaan virus necleocapsid and envelope protein studied using the baculovirus-expressed proteins." Archives of Virology. 130. 365-376 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoo, Y.-C., Yoshimatsu, K., Yoshida, R., Azuma, I.and Arikawa, J.: "Comparison of virulence between Seoul type virus strain SR-11 and Hantaantype virus strain 76-118 of hantaviruses in suckling mice." Microbiology and Immunology. 37. 557-562 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimatsu, K., Arikawa, J.and Kariwa, H.: "Application of recombinant baculovirus expressed hantavirus uncleocapsid protein as a diagnostic antigen in IFA test : cross reactive among three different serotypes of hemorrhagic fever with renal syndrome (HFRS)." Journal of Veterinary Medical Science. 55. 1047-1050 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arikawa, J.: "Hemorrhagic fever with renal syndrome (HFRS) ; Current topics, Immunological and diagnostic study." Laboratory Animal Technology and Science (in Japanese). 5(6). 277-281 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Isegawa, Y.: "Hemorrhagic fever with renal syndrome (HFRS) ; Current topics, Virology of the hemorrhagic fever with renal syndrome virus." Laboratory Animal Technology and Science (in Japanese). 5(6). 273-276 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arikawa, J.: "Epizootiological studies of hantavirus infection among urban rats in Hokkaido, Japan." Journal Veterinary Medical Science. 56. 27-32 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa, H., Arikawa, J., Takashima, I., Isegawa, Y., Yamanishi, K., Hashimoto, N.: "Enhancement of infectivity of hantavirus in cell culture by centrifugation" Journal of Virological Methods. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kariwa, H., Isegawa, Y., Arikawa, J., Takashima, I., Ueda, S., Yamanishi, K., Hashimoto, N.: "Comparison of nucleotide sequence of M genome segments among Seoul virus strains isolated from Easten Asia." Virus Research. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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