1992 Fiscal Year Final Research Report Summary
New viral diseases occurring in larval and juvenile marine fishes.
| Project/Area Number |
03660193
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
General fisheries
|
|---|
| Research Institution | HIROSHIMA UNIVERSITY |
|---|
Principal Investigator |
MUROGA Kiyokuni Faculty of Applied Biological Science, Hiroshima University, Professor, 生物生産学部, 教授 (30011993)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIZAWA Toyohiko Faculty of Applied Biological Science, Hiroshima University, Research Associate, 生物生産学部, 助手 (10222184)
NAKAI Toshihiro Faculty of Applied Biological Science, Hiroshima University, Associate Professor, 生物生産学部, 助教授 (60164117)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Keywords | Seed production / Viral disease / Striped jack / Redspotted grouper / Nodaviridae / ELISA / PCR |
|---|
| Research Abstract |
In this research project, viral epidermal hyperplasia(VEH) of flounder larvae and viral nervous necrosis(VNN) of striped jack larvae were studied. The results obtained are summarized as follows:
VEH A diagnostic measure using fluorescent antibody technique was developed for this viral infection.
VNN 1) The disease was confirmed to be caused by a virus and this virus (SJNNV) was identified to Nodaviridae based on its morphology and nucleic acid constitution. 2) A diagnostic method using ELISA was applied for this disease, and the detection of viral antigens from eggs indicates a vertical transmission of the disease. 3) The spawners of striped jack proved to be widely infected with the virus(SJNNV), and VNN occurred in larvae obtained from both antibody (anti-SJNNV)-positive and -negative spawners. 4) PCR (polymerase chain reaction) method was applied for the detection of SJNNV by using primers which were composed from analysis of RNA-2 sequence. Selection of virus-free eggs and larvae became possible by using this detection method.
|
