• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Ultrastructure and kinetics of the apical vesicles of the stria vascularis epithelial cell of the inner ear.

Research Project

Project/Area Number 03670012
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKochi Medical School

Principal Investigator

OKADA Teruhiko  Kochi Medical School, associate professor, 医学部, 助教授 (00025628)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Toshihiro  Kochi Medical School, 医学部, 助手 (40153621)
SEGUCHI Harumichi  Kochi Medical School, 医学部, 教授 (90030866)
Project Period (FY) 1991 – 1992
KeywordsStria vascularis / Cationized ferritin / Histochemistry / ACPase / TMPase / Na-K-ATPase / Ca-ATPase / Mg-ATPase
Research Abstract

The localization of ion-transporting enzyme activity and the endocytic process of cationized ferritin (CF) were electron microscopically investigated in the guinea pig stria vascularis.
Na-K-ATPase activity was localized in basolateral infolding of marginal cells of the sria vascularis. Ca-ATPase activity was detected in the limiting membrane of the large endosomal vacuoles and the luminal plasma membrane of the marginal cells. Mg-ATPase activity was found in the lateral plasma membrane of the marginal cells.
CF was first observed scattered on the luminal surface of the marginal cells. Then it was internalized in the form of coated vesicles and transported to the large vacuolar endosomes. At 5 min after CF injection, CF particles were mainly localized in the coated vesicles, and at 15 min, they appeared in large vacuoles. At 60 min, multivesicular bodies provided with several internal vesicles were havily labeled with CF.At 2 hours, the content of the multivesicular bodies condensed and internal vesicles, a granular substance of moderate to high density and membrane remants become clearly visible. These multivesicular bodies were havily labeled with clustered CF particles.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] T.Okada et al.: "Endocytosis of cationized ferritin in the marginal cells of the guinea pig stria vascularis." Journal of Electron Microscopy. 41. 320- (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Kanoh et al.: "Ultracytochemical study of ouabain-sensitive K^+-dependent p-nitrophenylphosphatase activity in stria vascularis of reserpinized guinea pig.in press" Acta Oto-laryngologica(Stockholm). 113. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Okada, T.Kobayashi, E.Garcia and H.Seguchi: "Endocytosis of cationized ferritin in the marginal cells of the guinea pig stria vascularis" Journal of Electron Microscopy. 41(4). 320 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Kanoh, T.Kumoi, T.Kobayashi, T.Okada and H.Seguchi: "Ultracytochemical study of ouabain-sensitive K^+-dependent p-nitrophenyl-phosphatase activity in stria vascularis of reserpinized guinea pigs" Acta Oto-laryngologica. 113, (in press). (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi