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1993 Fiscal Year Final Research Report Summary

The investigation of molecular mechanism of the activation of human basophils

Research Project

Project/Area Number 03670390
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

KAKUTA Yasunori  Tohoku University Hospital, Asistant, 医学部・附属病院, 助手 (80142933)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Kouhei  Tohoku University School of Medicine Asistant, 医学部, 助手 (20200579)
Project Period (FY) 1991 – 1993
KeywordsBasophils / Bronchial Asthma / Degranulation / Calcium ion / G-Protein
Research Abstract

We applied a patch-clamp technique to highly purified human basophils and examined the effect of intracellular Ca concentration ([Ca^<2+>]_i) on granule release by morphological and electrophysiological measurement. In addition, we examined the effect of guanosine 5'-_0-(3-thiortriphosphate) (GTP-gamma-S) and a specific protein kinase C inhibitor, calphostin C, on intracellular Ca-induced granule release. We obtained the following results : First, intracellular application of Ca, 1-10muM, caused granule fusion, which was confirmed by the definite correlation between morphological and electrophysiological changes. The addition of GTP-gamma-S,100muM, promoted granule release by 4.4 times at 2muM Ca in association with marked morphological changes. In addition, GTP-gamma-S caused significant granule membrane fusion even at 0.1muM Ca. Calphostin C, 200nM, inhibited intracellular Ca-and intracellular Ca+GTP-gamma-S-induced granule release by 69% and 50%, respectively. These results indicate that granule membrane fusion is induced by an increase in intracellular Ca alone at relatively high concentrations in the human basophil. In addition, GTP binding proteins and protein kinase C would modulate the Ca-induced granule release.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Toshiya Aizawa: "Induction of granule release by intracellular application of calcium and guanosine-5'-o-(3-thiotriphosphate)in human eosinophils." J.Allergy Clin.Immunol.90. 789-795 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobuhiro Maruyama: "Quinine inhibits production of tumor necrosis factor-α from human alveolar macrophages." Am.J.Respir.Cell Mol.Biol.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiya Aizawa et al.: "Induction of granule release by intracellular application of calcium and guanosine-5'-o-(3-thiotriphosphate) in human eosinophils" J.Allergy Clin. Immunol. 90. 789-795 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nobuhiro Maruyama et al.: "Quinine inhibits production of tumor necrosis factor-alpha from human alveolar macrophages." Am.J.Respir. Cell Mo1. Bio1.in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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