1992 Fiscal Year Final Research Report Summary
Effect of chemotactic factor on pulmonary vascular permeability
| Project/Area Number |
03670396
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | SHINSHU UNIVERSITY SCHOOL OF MEDICINE |
|---|
Principal Investigator |
YOSHIMURA Kazuhiko Shinshu Univ., Assistant Prof., 医学部附属心脈管病研究施設, 講師 (70174985)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUBO Keishi Shinshu Univ., Assistant Prof., 医学部, 講師 (80143965)
KOBAYASHI Toshio Shinshu Univ., Assistant Prof., 医学部附属病院, 講師 (80020775)
HANDA Kenjiro Shinshu Univ., Associate Prof., 医学部, 助教授 (70020724)
|
|---|
| Project Period (FY) |
1991 – 1992
|
| Keywords | chemotactic factor / leukotriene B4 / polymorphonuclear leukocyte / pulmonary vascular permeability / filtration coefficient / isolated perfused lungs / wet to dry lung weight ratio / pulmonary edema |
|---|
| Research Abstract |
The effects of exogenous LTB4 on the pulmonary microvascular perme-ability and the roles of polymorphonuclear leukocytes (PMNs), cyclooxy-genase products of arachidonic acid and neutrophil elastase in the microvascular response to LTB4 in the isolated non-blood perfused rabbit lungs were studied. We observed that LTB4 increased filtration coefficient (Kf) and wet to dry lung weight ratio (W/D ratio) in the presence of PMNs in the perfusate. Neither LTB4 alone nor PMNs alone produced changes in Kf and W/D ratio. Indomethacin failed to inhibit the LTB4-induced increases in Kf and W/D ratio. Neutrophil elastase inhibitor effectively inhibited the LTB4-induced lung injury. These results suggest that LTB4 produces lung injury which is dependent on neutrophil elastase derived from PMNs but not on the cyclooxygenase pathway of arachidonic acid metabolism.
|
Research Products
(4 results)
