1993 Fiscal Year Final Research Report Summary
Analysis of the T-cells for the specific adoptive immunotherapy.
| Project/Area Number |
03670613
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
MASAKI Tadahiko Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30238894)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MUTO Yasuhiko Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 医員
SANO Talesjo Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 助手 (30215884)
NAGAWA Hirokazu Faculty of Med, University of Tokyo, Assistant Professor, 医学部(病), 講師 (80228064)
KOBORI Ouichiro Faculty of Med, University of Tokyo, Associate Professor, 医学部(病), 助教授 (30110696)
|
|---|
| Project Period (FY) |
1991 – 1993
|
| Keywords | Tumor-draining lymphnode / Cytotoxicity / CD-8 / Adoptive immunotherapy / Anti-CD3 antibody / Interleukin-2 / Interferon-gamma |
|---|
| Research Abstract |
Tumor-draining lymphnode (DLN) cells, obtained from C57BL/6 mice with fibrosarcoma (MC-1), were activated and expanded with anti-CD3 monoclonal antibody followed by the culture with recombinant interleukin-2 These cells were non-cytolytic in vitro. However, they showed the specific anti-tumor efficacy to the pulmonary micrometastases of MC-1. The supernatant, obtained by co-culturing the activated DLN cells with MC-1, exhibited the specific production of interferon-gamma(IFN-gamma). The therapeutic effect correlated with the specific IFN-gamma production better than the cytolytic activity.
|
