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1993 Fiscal Year Final Research Report Summary

Tonsil and Free Radical

Research Project

Project/Area Number 03670814
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionSapporo Medical University

Principal Investigator

KATAURA Akikatsu  Sapporo Medical University, Otolaryngology, Professor, 医学部, 教授 (60045359)

Co-Investigator(Kenkyū-buntansha) KUKUMINATO Yasushi  Sapporo Medical University, Otolaryngology, Instructor, 医学部, 助手 (00231291)
SHIDO Fumiaki  Sapporo Medical University, Otolaryngology, Lecturer, 医学部, 講師 (70170876)
Project Period (FY) 1991 – 1993
Keywordstonsil / focal tonsillitis / superoxide / cytochrome C / SOD / ASO / S.pyogenes / tonsillitis
Research Abstract

The tonsil plays an important role in immune response against many foreign antigens, and could be the target organ for the bacterial infection. It is well known that neutrophils which shows bactericidal function due to product the superoxide. The purpose of our research is to clarify the patho-physiology of tonsillitis. And we investigated the application of that to the usual clinical medicine.
1. Tonsillar T- and B-lymphocytes were isolated from patients with recurrent tonsillitis or focal tonsillitis by Ficoll-Conray gradient method and E-rosette forming method. The SOD (superoxide dismutase) activity of T- and B-lymphocytes was measured, and the activity of T-lymphocytes tended to be higher than that of B-lymphocytes.
2. Peripheral neutrophils were collected from patient with recurrent tonsillitis or focal tonsillitis, and the superoxide productions of these cells were measured with cytochrome C method. There was no significant correlation between superoxide productions and CRP,peripheral WBC cound and frequency of tonsillitis, however, the superoxide productions related to the serum titer of ASO (antistreptolysin O) negatively (r=-.44, p=.002).
3. Superoxide productions of neutrophils cultured with the supernatant culture of S.pyogenes tended to be inhibited with protein concentration of the supernatant dependent manner.
These result indicated that recurrent Streptococcul infections may inhibit the superoxide productions of the neutrophils in recurrent tonsillitis or focal tonsillitis.
Further study is needed to investigate what components of the S.pyogenes supernatant effect the superoxide productions and the relationship between the superoxide and tonsillar lymphocytes.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 横山雄司、久々湊靖、形浦昭克: "扁桃とフリーラジカルについての研究(第1報)" 第32巻 日本扁桃研究会誌. 32. 36-39 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横山雄司、原渕保明、久々湊靖、形浦昭克: "習慣扁桃炎患者における末梢血顆粒球フリーラジカル産生能" 第6回 日本口腔・咽頭科学会発表. 9月3日. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横山雄司、原渕保明、久々湊靖、形浦昭克: "習慣扁桃炎患者における好中球スーパーオキサイド産生能の検討" 日本耳鼻咽喉科学会会報. 97. 2259-2265 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yokoyama, Y.Harabuchi, Y.Kukuminato, A.Kataura: "Superoxide Production by Neutrophils in Patients with Recurrent Tonsillitis" Juornal of Otolaryngology of Japan. 97. 2259-2265 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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