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1992 Fiscal Year Final Research Report Summary

Production of New Bioactive Compounds in Transgenic Medicinal Plants.

Research Project

Project/Area Number 03807138
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Chemical pharmacy
Research InstitutionChiba University

Principal Investigator

MURAKOSHI Isamu  Chiba Univ., Fac. Pharm. Sci. Professor, 薬学部, 教授 (30009162)

Co-Investigator(Kenkyū-buntansha) YAMAZAKI Mami  Chiba Univ., Fac. Pharm. Sci. Res. Assist., 薬学部, 教務職員 (70222370)
SAITO Kazuki  Chiba Univ., Fac. Pharm. Sci. Lecturer, 薬学部, 講師 (00146705)
Project Period (FY) 1991 – 1992
Keywordstransgenic plant / secondary metabolism / herbicide-resistance / inheritance of transgenic trait / molecular breeding
Research Abstract

Transgenic herbicide-resistant plants of Scoparia dulcis were obtained by using Ri binary vector system. The chimeric bar gene encoding phosphinothricin acetyltransferase flanked with promoter for cauliflower mosaic virus 35S RNA and terminal sequence for nopaline synthase was introduced in the plant genome by scratching young plants. Hairy roots resistant to bialaphos were selected and plantlets (R0) were regenerated. Progenies (S1) were obtained by selffertilization. Transgenic state was confirmed by DNA-blot hybridization and enzyme assay of neomycin phosphotransferase II. Expression of bar gene in transgenic R0 and S1 progenies was indicated by enzymatic activity of phosphinothricin acetyltransferase. Transgenic plants accumulated scopadulcic acid B, a specific secondary metabolite of S. dulcis, by the amounts of 15-60% compared with that of normal plants. The transgenic plants and progenies showed resistance towards bialaphos and phosphinothricin. These results suggest that Ri binary system is one of the useful tools for transformation of medicinal plants in which regeneration protocol is not established.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] K.SAITO他: "Transgenic herbicide-resistant Atropa belladonna using an Ri binary vector and inheritance of transgenic trait." Plant Cell Reports. 11. 219-224 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.SAITO他: "Transgenic medicinal plants:Agrobacterium-mediated foreign gene transfer and production of secondary metabolites." Journal of Natural Products. 55. 149-162 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.SAITO他: "Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase." Proceedings of National Academy of Sciences USA. 89. 8078-8082 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.SAITO他: "Isolation and nucleotide sequence of cDNA encoding cysteine synthase from spinach." Plant Physiology.99S. 148 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 斉藤 和季: "トランスジェニック薬用植物の作出と物質代謝の修飾" 組織培養. 18. 407-411 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.SAITO他: "Acyltrans for lupin alkaloidds in Lupinus hirsutus" Phytochemistry. 32. 87-91 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.SAITO他(分担執筆): "Plant Tissue Culture and Gene Manipulation for Breeding and Formation of Phyto chemicals(ed.by Oono et al.)" NIAR/Japan, 395 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山崎 真巳他(分担執筆): "「植物バイオテクノロジーの基礎と産業化」" 日本植物培養学会, 157 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K. Saito, M. Yamazaki, H. Anzai, K. Yoneyama, I. Yamaguchi and I. Murakoshi: "Transgenic herbicide-resistant Atropa belladonna using an Ri binary vector and inheritance of transgenic trait." Plant Cell Reports,. 11. 219-224 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito, M. Yamazaki and I. Murakoshi:"Transgenic medicinal plants: Agrobacterium-mediated foreign gene transfer and production of secondary metabolites." Journal of Natural Products. 55. 149-162 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito, N. Miura, M. Yamazaki, H. Hirano and I. Murakoshi: "Molecular cloning and bacterial expression of cDNA encoding a plant cysteine synthase." Proceedings of National Academy of Sciences USA. 89. 8078-8082 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito, N. Miura, M. Yamazaki and I. Murakoshi:"Isolation and nucleotide sequence of cDNA encoding cysteine synthase from spinach." Plant Physiology. 99S. 148 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito:"Genetic Modification of Metabolism in Transgenic Medicinal Plants." The Tissue Culture. 18. 407-411 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K. Saito, H. Suzuki, S. Takamatsu and I. Murakoshi: "Acyltransferases for lupin alkaloids in Lupinus hirsutus." Phytochemistry. 32. 87-91 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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