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1993 Fiscal Year Final Research Report Summary

Endemic Primary Distal Renal Tubular Acidosis in Thailand

Research Project

Project/Area Number 04041041
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionKyorin University School of Medicine (1993)
The University of Tokyo (1992)

Principal Investigator

ENDOU Hitoshi  Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 医学部, 教授 (20101115)

Co-Investigator(Kenkyū-buntansha) VIPADA Chaovakul  Department of Medicine Ubol District Hospital, 内科学, 医長
WATTANACHAI スサエングラット  コンケン地区病院, 内科学, 医長
UDOM Chantharaksri  Department of Pharmacology, Faculty of Science, Mahidol University, 理学部, 助手
CHAIRAT Shayakul  Department of Medicine, Faculty of Medicine, Siriraj Hospital, 医学部, 助手
SAMAISUKH So  マヒドン大学, 理学部, 講師
SOMKIAT Vasuvattakul  Department of Medicine, Faculty of Medicine, Siriraj Hospital, 医学部, 助手
PRIDA Malasit  Department of Molecular Biology, Faculty of Medicine Mahidol University, 医学部, 講師
SUMALEE Nimm  マヒドン大学, 医学部, 教授
SANGA Nilwarangkur  Department of Medicine, Faculty of Medicine, Siriraj Hospital, 医学部, 教授
MURAI Yoshiro  Department of Hematology, Tokyo Metropolitan Tama Geriatric Hospital, 血液科, 医長
CHIBA Momoko  Department of Hygiene, Juntendo University School of Medicine, 医学部, 助教授 (80095819)
KOMATSU Yasuhiro  Department of Pediatric Nephrology, Tokyo Women's Medical College, 医学部, 助手 (60195849)
INABA Yutaka  Department of Hygiene, Juntendo University School of Medicine, 医学部, 教授 (30010094)
IGARASHI Takashi  Department of Pediatrics, Faculty of Medicine The University of Tokyo, Mejirodai, 医学部, 講師 (70151256)
SUMALEE Nimmannit  Department of Medicine, Faculty of Medicine, Siriraj Hospital
SAMAISUKUH Sophasan  Department of Physiology, Faculty of Science, Mahidol University
WATTANACHAI Susaengrat  Department of Medicine, Khon Kaen District Hospital
Project Period (FY) 1992 – 1993
KeywordsDistal renal tubular acidosis / North-eastern Thailand / beta2-Microglobulin / Hypokalemia / Hypomagnesemia / Proximal tubule dysfunction / Trace element / Sodium pump
Research Abstract

The purpose of this project was to investigate possible causes of endemic renal distal tubular acidosis(EdRTA)in Northeastern part of Thailand. Two ways of trials were carried out, fieldwork and animal model experiments. Results obtained are as follows.
1.Fieldwork : A research center has been settled at the Ubol District Hospital, Ubol Rajathanee, Ubol Prefecture, Thailand. EdRTA patients and healthy volunteers were selected for interview, clinical examinations and clinical biochemical analyses of blood and urine. Materials such as diet, soil, water and agricultural products were also sampled and brought to Japan for analysis of trace elements. Results are as follows.
(1)Most patients were old aged females. They were small in height and light in body weight. Thus, BMI (body weight/hight^2)were low. Their urine gravity decreased.
(2)Urinary beta2-microglobulin contents in EdRTA were significantly increased, and pH was high. Urinary creatinine concentrations decreased.
(3)Urinary vanadium e … More xcretion was significantly increased in EdRTA.
(4)Plasma Mg concentrations were high, and K was low in both sexes. Zn concentrations were high in male EdRTA, and Na was low in the female.
2.Animal model with low Mg diet
Mg is an essential substrate especially for ATPases including Na, K-ATPase and H-ATPase, and low Mg sometimes is parallelled with low K. Usually Mg is reabsorbed in the thick ascending limb of Henle's loop(TAL). We, therefore, microdissected TAL and the collecting tubule(CT)from control and low K rat kidneys for Na, K-ATPase assay.
(1)Na, K-ATPase activity in TAL from low Mg rats was significantly lowered.
(2)The enzyme activity in CT was significantly increased. This finding is unexpected, since lowered Na, K-ATPase activity should initiate plasma K increase, resulting in dRTA theoretically. This discrepancy should be further investigated.
3.Analysis of urinary proteins in hypo K rats.
SD rats were treated with low K diet for 3 months, and their urinary proteins were electrophoresed. Total protein excretion was significantly increased. Electrophoretic patterns show increment of not only low molecular proteins but also high molecular proteins. This result is also slightly different from clinical data.
In conclusion, these results clearly suggest that electrolytes and/or trace elements may play important roles in manifestation of EdRTA in Thailand. Less

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] T.Igarashi,Y.Inaba,M.Chiba,Y.Komatsu,Y.Murai,H.Endou,S.Nilwarangkur,S.Nimmannit,V.Chaovakul: "Low-molecular-weight proteinuria in patients with endemic distal renal tubular acidosis in the northeast of Thailand" Kidney International.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Igarashi,T.Kosugi: "The Incidence of Renal Cyst Formation in Patients with Primary Distal Renal Tubular Acidosis" Nephron. 66. 474 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Igarashi,T.Ishii,K.Watanabe,H.Hayakawa,K.Horio,Y.Sone,and K.Ohga: "Persistent isolated proximal renal tubular acidosis-a systemic disease" Pediatr Nephrol. 8. 70-71 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Igarashi,Y.Sekine,H.Kawato,S.Kamoshita,Y.Saigusa: "Transient neonatal distal renal tubular acidosis with secondary hyperparathyroidism" Pediatr Nephrol. 6. 267-269 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Komatsu,K.Ito,H.Endou: "Effect of Magnesium Depletion on Na,K-ATPase activity of rat Nephron segments" J Am Soc Nephrol.4. 710 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Igarashi, Y.Inaba, M.Chiba, Y.Komatsu, Y.Murai, H.Endou, S.Nilwarangkur, S.Nimmannit, V.Chaovakul: "Low-molecular-weight proteinuria in patients with endemic distal renal tubular acidosis in the northeast of Thailand" Kidney International.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Igarashi, T.Kosugi: "The Incidence of Renal Cyst Formation in Patients with Primary Distal Renal Tubular Acidosis" Nephron. 66. 474 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Igarashi, T.Ishii, K.Watanabe, H.Hayakawa, K.Horio, Y.Sone, and K.Ohga: "Persistent isolated proximal renal tubular acidosis-a systemic disease with a distinct clinical entity" Pediatr Nephrol. 8. 70-71 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Igarashi, Y.Sekine, H.Kawato, S.Kamoshita, Y.Saigusa: "Transient neonatal distal renal tubular acidosis with secondary hyperparathyroidism" Pediatr Nephrol. 6. 267-269 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Komatsu, K.Ito, H.Endou: "Effect of Magnesium Depletion on Na, K-ATPase activity of rat nephron segments" J Am Soc Nephrol.4. 710 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-02-07  

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