Comparison of Photochemistry for various retinal-proteins : Consideration for relationship between their structure and function

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04302065
• Research Category: Grant-in-Aid for Co-operative Research (A)
• Allocation Type: Single-year Grants
• Research Field: 生物物性学
• Research Institution: Hokkaido University
• Principal Investigator: KAMO Naoki Hokkaido University, Faculty of Pharmaceutical Science, Professor, 薬学部, 教授 (10001976)
• Co-Investigator(Kenkyū-buntansha): TOKUNAGA Fumio Osaka University, Faculty of Science, Professor, 理学部, 教授 (80025452) ; KITAGAWA Teizo Okazaki National Research Institutes, Institute for Molecular Science, 分子科学研究所, 教授 (40029955) ; TASUMI Mitsuo The University of Tokyo, Faculty of Science, Professor, 理学部, 教授 (60011540) ; SUZUKI Hideo Waseda University, School of Science and Engineering, Professor, 理工学部, 教授 (60063541) ; MUKOHATA Yasuo Nagoya University, Faculty of Science, Professor, 理学部, 教授 (10028110)
• Project Period (FY): 1992 – 1993
• Keywords: Rhodopsin / Bacteriorhodopsin / Halorhodopsin / Phoborhodopsin / Paramecium / Chlamydomonas / Halobacteria / Photochemistry

Research Abstract

(1) Photochemistry of pharaonis phoborhodopsin (ppR) was made clear and the structure of binding pocket of retinal was estimated.
(2) Mutant retinal proteins were obtained both from naturallyhabitant halobacteria and by artificial DNA.Characterization gave clues on amino acids essential for the function.
(3) Octopus rhodopsin was well characterized.
(4) Structural changes of bacteriorhodopsin, Octopus rhodopsin were investigated by FTIR and by Raman spectroscopy.
(5) Crystallization of Squid rhodopsin and bacteriorhodopsin were tried and interesting findings were obtained.
(6) Interference of phototaxis of Chlamydomonas by photosynthesis was first showed. Rhodopsin-like proteins and mechanism of photo-sensory were clarified.
(7) Molecular dynamic theory of retinal isomerization and analysis of vibration mode of retinal were developed.

Research Products

• [Publications] Takahashi,T.et al.: "Photosynthesis modulates the sign of phototaxis of wild-type Chlamydomonas reinhardtii" FEBS Lett.336. 516-520 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kubo,I.,et al.: "A new type of halide-ion biosensor using halorhodopsin and an ion-sensitive field-effect transistor." Sensors and Actuators. B13-14. 659-660 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kandori,H.,et al.: "Comparative study of primary photochemical events of two retinal proteins,bacteriorhodpsin and halorhodopsin." Chem.Phys.Lett.211. 385-391 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takahashi,S.,et al.: "Multichannel Fourier transform spectroscopy using two-dimensional detection of interferogram and application to Raman spectroscopy." Appl.Spectrosc.47. 863-868 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizutani,Y.,et al.: "Ultraviolet resonance Raman spectra of pea intact,large,and small phytochromes" Biochemistry. 32. 6916-6922 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Masuda,S.et al.: "Infrared Study of Octopus Rhodopsin Existence of a Long-lived intermediate and the States of the Carboxylic Group of Asp-81 in Rhodopsin and its Photoproducts." FEBS Lett.317. 223-227 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Seidou,M.et al.: "Nucleotide content of Octopus photoreceptor cells;No changes in octopus retina immediately following an intense light flash." Zool.Sci.10. 275-279 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Suzuki,T.et al.: "Immunochemical detection of GTP-binding protein in cephalopod photoreceptors by anti-peptide anti-bodies." Zool.Sci.10. 425-430 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sasaki,J.et al.: "Time-resolved infrared spectral analysis of the KL-to-L conversion in the photocycle of bacteriorhodopsin." Biochemistry. 32. 867-871 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Maeda,A.: "Structure changes of bacteriorhodopsin in the L-to-M and M-to-N conversions." Frontiers of Photobiology. 135-138 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Araga,C.et al.: "Functional reconstitution of the primary quinone acceptor,OA,in the photosystem II core complex." Biochim.Biophys.Acta. 1142. 36-42 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Maeda,A.,et al.: "Water structural changes in lumirhodopsin,metarhodopsin I,and metarhodopsin II upon photolysis of bovine rhodopsin" Biochemistry. 32. 12033-12038 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Masuda,S.et al.: "Infrared Study of Octopus Rhodopsin and Lumirhodopsin." J.Mol.Struct.297. 29-34 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Suzuki,H.et al.: "Dynamical theory of the photoisomerization of visual-pigment chromophore(II)" Bull.Sci.Eng.Res.Lab.,Waseda Univ.142. 79-89 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kikkawa,S.et al.: "GTP-binding Protein Couples with Metabotropic Glutamate Receptors in Bovine Retinal ON-Bipolar Cell." Biochem.Biophys.Res.Commun.195. 374-379 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Taiji,M.et al.: "Femtosecond Studies of Primary Photoprocesses in Octopus Rhodopsin." Photochem.Photobiol.56. 1003-1011 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kouyama,T.: "Bacteriorhodopsin,a bilogical proton pump working in the ligh." RIKEN Review. 1. 5-6 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Uehara,K.et al.: "Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alcohol)film." Thin Solid Films. 232. 271-277 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mukohata,Y.: "Comparative studies on bacterial rhodopsins-new pumps and new study." Biophys.Chem.(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sugiyama,Y.and Mukohata,Y.: "Archaeopsin expressed in Escherichia coli and its conversion to purple pigment in vitro." J.Biochem.(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mizutani,Y.et al.: "Resonance Raman spectra of the photointermediates in phytochrome phototransformation" Biochemistry. 33. 153-158 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kouyama,T.et al.: "Polyhedral assembly of a membrane protein in its three-dimensional crystal." J.Mol.Biol.236(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hamanaka,T.,et al.: "X-ray study of the live squid retina." J.Mol.Biol.(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Maeda,A.,et al.: "Interaction of aspartate-85 with a water molecule and the protonated Schiff base in the L intermediate of bacteriorhodopsin" Biochemistry. 33(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakaki,J.et al.: "Complete identification of C=0 stretching vibrational bands of protonated aspartic acid residues in the difference infrared spectra of M and N intermediates versus bacteriohrodopsin." Biochemistry. 33(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kimura,N.et al.: "Time-resolved fluorescence depolarization to study the molecular motion of organic thin films." Langmuir. (in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takahashi, T.and W.Watanabe: "Photosynthesis modulates the sign of phototaxis of wild-type chlamydomonas reinhardtii : Effects of red backgroud illumination and 3-(3', 4'-dichloropheny1)-1,1-dimethylurea." FEBS Lett.336. 516-520 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kubo, I., Seki, A., Tomioka, H., Sasabe, H.: "A new type of halide-ion biosensor using halorhodopsin and an ion-sensitive field-effect transistor." Sensors and Actuators. B 13-14. 659-660 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kandori, H., Yoshihara, K., Tomioka, H., Sasabe, H.and Shichida, Y.: "Comparative study of primary photochemical events of two retinal proteins, bacteriorhodopsin and halorhorhodopsin, by use of subpicosecond time-resolved spectroscopy." Chem.Phys.Lett.211. 385-391 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hariyama, T., Ozaki, K., Tokunaga, F.and Tsukahara, Y.: "Primary structure of crayfish visual pigment deduced from cDNA." FEBS lett.315. 287-292 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kataoka, M., Mihara, K., Kamikubo, H., Lanyi and Tokunaga, F.: "Trimeric mutant Bacteriorhodopsin, D85N, shows a monomeric CD spectrum." FEBS lett.333. 111-113 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Takahashi, S., Ahn, J.S., Asaka, S.and Kitagawa, T.: "Multichannel Fourier transform spectroscopy using two-dimensional detection of interferogram and its application to Raman spectroscopy." Appl.Spectrosc.47. 863-868 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mizutani, Y., Tokutomi, S., Kaminaka, S.and Kitagawa, T.: "Ultraviolet resonance Raman spectra of pea intact, large, and small phytochromes : Differences in molecular topography of the red and farred absorbing forms." Biochemistry. 32. 6916-6922 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Masuda, S., Morita, E.H., Tasumi, M., Iwasa, T.and Tsuda, M.: "Infrared Study of Octopus Rhodopsin : Existence of a Long-lived Intermediate and the States of the Carboxylic Group of Asp-81 in Rhodopsin and its Photoproducts." FEBS Lett.317. 223-227 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Masuda, S., Morita, E.H., Tasumi, M., Iwasa, T.and Tsuda, M.: "Infrared Study of Octopus Rhodopsin and Lumirhodopsin." J.Mol.Struct.297. 29-34 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Suzuki, H., Kikuchi, H.and Shimabukuro, T.: "Dynamical theory of the photoisomerization of visual-pigment choromophore (II) -How to calculate the integrals related with transient electronic states-." Bull.Sci.Eng.Res.Lab., Waseda Univ.142. 79-89 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kikkawa, S., Nakagawa, M., Iwasa, T., Kaneko, A.and Tsuda, M.: "GTP-binding protein Couples with Metabotropic Glutamate Receptors in Bovine Retinal ON-Bipolar Cell." Biochem.Biophys.Res.Commun.195. 374-379 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kouyama, T.: "Bacteriorhodopsin, a biological proton pump working in the ligh." RIKEN Review. 1. 5-6 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Uehara, K., Kawai, K.and Kouyama, T.: "Photoelectric response of oriented purple membrane electrodeposited onto poly(vinyl alchol) film." Thin Solid Films. 232. 271-277 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Seido, M., Ohtsu, K., Narita, K.and Kito, Y.: "Nucleotide con-tent of Octopus photoreceptor cells ; No changes in octopus retina immediately following an intense light flash." Zool.Sci.10. 275-279 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Suzuki, T., Narita, K.and Kito, Y.: "Immunochemical detection of GTP-binding protein in cephalopod photoreceptors by anti-peptide anti-bodies." Zool.Sci.10. 425-430 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sasaki, J., Maeda, A., Kato, C.and Hamaguchi, H.: "Time-resolved infrared spectral analysis of the KL-to-L conversion in the photocycle of bacteriorhodopsin." Biochemistry. 32. 867-871 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Maeda, A.: "Structure changes of bacteriorhodopsin in the L-to-M and M-to-N conversions." Frontiers of Photobiology. 135-138 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Araga, C., Akabori, K., Sasaki, J., Maeda, A., Shiina, T.and Toyoshima, Y.: "Functional reconstitution of the primary quinone acceptor, QA, in the photosystem II core complex." Biochim.Biophys.Acta. 1142. 36-42 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Maeda, A., Ohkita, Y.J., Sasaki, J., Shichida, Y.and Yoshizawa, T.: "Water structural changes in lumirhodopsin, metarhodopsin I, and metarhodopsin II upon photolysis of bovine rhodopsin : Analysis by Fourier transform infrared spectroscopy." Biochemistry. 32. 12033-12038 (1993)
  • Description: 「研究成果報告書概要(欧文)」より