1993 Fiscal Year Annual Research Report

鞭毛・繊毛運動を制御する分子調節機構の解明

Research Project

Project/Area Number	04304006
Research Institution	University of Tokyo
Principal Investigator	森沢正昭東京大学, 理学部, 教授 (40013594)
Co-Investigator(Kenkyū-buntansha)	奥野誠東京大学, 教養学部, 助教授 (40143325) 豊島陽子東京大学, 教養学部, 助教授 (40158043) 神谷律東京大学, 理学部, 教授 (10124314) 高橋景一国際基督教大学, 教養学部, 教授 (40011481) 毛利秀雄放送大学, 教養学部, 教授 (70012268)
Keywords	鞭毛・繊毛運動調節 / 微小管滑り / ダイニン / 再構成運動系 / モータードメイン / 遺伝子解析 / プロテアソーム / タンパク質リン酸化
Research Abstract	高橋は断片化したウニ精子鞭毛軸糸にエラスターゼを作用させ、ATP中で滑り運動を解析し、滑り速度のCa^<2+>依存性を示した。真行寺は除膜ウニ精子で、振動数を変えて鞭毛微小管滑り速度を解析し、滑り速度の振動数に伴う変化は外腕を抽出しても起きることを示した。豊島はキネシン頭部の遺伝子を用い特定部位を修飾し、頭部のみで微小管を滑らせる能力があることを明らかにした。小川はダイニンβ重鎖機能ドメインを、その相当領域をコードするcDNAをベクターにつなげ、バキュロウイルス発現系を用い量産する方法を確立した。神谷はクラミドモナスでダイニン外腕β鎖が短い変異株、内腕重鎖一部欠失株を単離、解析し、外腕の機能にβ重鎖が重要で、内腕重鎖の機能が重複していることを示した。毛利は系統上重要な腔腸動物イソギンチャクで精子鞭毛外腕ダイニンを生化学的・超微形態学的に検討し、2頭構造であることを示した。中村はキモトリプシン感受性の著しく異なるαチューブリン由来のチューブリンアイソフォームを見い出した。森沢は海産魚精子運動開始に細胞内イオン強度の増加が必要であることを示し、更にニシン精子活性化物質のcDNAクローニングを完成した。稲葉はシロサケ精子で、プロテアソームが鞭毛軸糸と細胞膜を連結している構造に局在していることを免疫電顕法により明らかにした。奥野は哺乳類精子で、繊維鞘の滑りが微小管#5-6のそれに伴って起きることから#5-6のダイニンの活性が高いことを示した。中岡は繊毛波の形成をcGMPが調節していることを示した。野口はゾウリムシ繊毛の有効打の方向制御機構に、29,65kDaタンパクのcAMP依存的リン酸化が重要であることを示した。馬場は精子鞭毛断片の周波数測定で、高Ca^<2+>でオシレーター2が特異的に阻害されることを示した。

Research Products
(52 results)

All Other

All Publications (52 results)

[Publications] Morisawa,M.: "Regulatory mechanisms of sperm motility." In Biology of the germ line.In animals and man.(H.Mohri,M.Takahashi,and C.Tachi,eds.). 81-93 (1993)
[Publications] Morisawa,S.: "Roles of pH and cyclic adenosine monophosphate in the aquisition of potential for sperm motilityduring migration from the river in chum salmon." Mol.Rep.Dev.34. 420-460 (1993)
[Publications] Oda,S.: "Rises of intracelular Ca^<2+> and pH mediate the initiation of sperm motility by hyperosmolality in marine teleosts." Cell Motil.Cytoskeleton.25. 497-506 (1993)
[Publications] Yoshida.M.: "Sperm chemotaxix during the process of fertilization in the ascidians Ciona savygni and Ciona intestinalis." Dev.Biol.157. 497-506 (1993)
[Publications] Yamada,A.: "Sudden increase in speed of an filament moving on myosin cross-bridges of“mismatched"polarity observed when its leading end begins to interact with cross-bgidges of“matched"polarity." J.Biochem.111. 676-680 (1992)
[Publications] Murakami,A.: "Analysis of gravitational responce of Paramecium by free-all experiments." Proc.of the 9th Space Utilization Symp.102-104 (1992)
[Publications] Murakami,A.: "Analysis of gravitation and response to microgravity in Paramecium." Proc.of the 10th Space Utilization Symp.86-87 (1993)
[Publications] 高橋景一: "鞭毛運動" Annual Review細胞生物学1993. 165-178 (1993)
[Publications] Eshel,D.: "The phase of sperm flagellar beating is not conserved over a brief imposed interruption." Exp.Cell Res.202. 552-555 (1992)
[Publications] Ogawa,K.: "Primary structure and function of a dynein motor molecule." Zool.Sci.9. 265-274 (1992)
[Publications] 小川和男: "鞭毛モーターダイニンの分子構造" Cell Science. 9. 33-41 (1993)
[Publications] Ogawa,K.: "cDNA cloning for mouse caltractin" Biochim.Biophys.Acta.1216. 126-128 (1993)
[Publications] Kamimura,S.: "High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin." J.Cell Biol.116. 1443-1454 (1992)
[Publications] Kamiya,R.: "Molecular mechanism of ciliary and flagellar movement" Advances in Comparative and Enviromental Physiology. 12. 206-226 (1992)
[Publications] Takada,S.: "Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer arm-missing axonemes." J.Biochem.111. 758-762 (1992)
[Publications] Kagami,O.: "Translocation and lotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein." J.Cell Biol.103. 653-664 (1992)
[Publications] Sakakibara,H.: "A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain." J.Cell Biol.122. 653-662 (1993)
[Publications] Kato,T.: "Isolation of two species of Chlamydomonas reinhardtii flagellar mutants,ida5 and ida6,that lack a newly identified heavy chain of the inner dynein arm" Cell Structure and Function. (印刷中). (1994)
[Publications] Johara,M.: "Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion" Proc.Natl.Acad.Sci.USA.90. 2127-2131 (1993)
[Publications] Itakura,S.: "Force-generating domain of myosin motor." Biochem.Biophys.Res.Commun.196. 1504-1510 (1993)
[Publications] 豊島陽子: "in vitro運動系" Annual Review細胞生物学1993. 3. 145-155 (1993)
[Publications] Iwamoto,T.: "Purification and characterization of a sperm motility-dynein ATPase inhibitor from boar seminal plasma." Mol.Reprod.Deveop.31. 55-62 (1992)
[Publications] Wada,S.: "Dynein of sperm flagella of oyster belonging to Protostomia also has a two headed structure" Biol.Cell. 76. 311-317 (1992)
[Publications] Mohri,H.: "Role of tubulin and dynein in spermatozoan motility" Mol.Reprod.Develop.36. 221-223 (1993)
[Publications] 高谷知恵子: "電気泳動法を応用したタンパク質の分離精製" 比較生理生化学. 9. 156-159 (1992)
[Publications] Wazawa,T.-I.: "Hydrogen peroxide plays a key role in the oxidation reaction of myoglobin by molecular oxygen." Biophys.J.63. 544-555 (1992)
[Publications] Jin,Z.X.: "Isolation and properties of a protein complex that cantains flagellar movement initiating-phosphoprotein from testis of chum salmon." J.Biochem.(印刷中). (1994)
[Publications] Jin,Z.X.: "Monoclonal antibodies against the protein complex that contains the flagellar movement initiating-phosphoprotein of Onchorhynchus keta." J.Biochem.(印刷中). (1994)
[Publications] 森沢正昭: "特集を終えるにあたって" Cell Science. 9. 1993 (1992)
[Publications] Inaba,K.: "Two high molecular mass proteases from sea urchin sperm" Biochem.Biophys.Res.Commun.182. 667-674 (1992)
[Publications] Inaba,K.: "Chymotrypsin-like protease activity associated with demembranated chum salmon sperm." Biol.Cell. 76. 329-333 (1992)
[Publications] 稲葉一男: "精子のプロテアソーム" Cell Science. 8. 750-758 (1992)
[Publications] Inaba,K.: "Interactions among the heterogenous components of dynein" In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.76. 385-386 (1992)
[Publications] Inaba,K.: "Purification of fproteasomes from salmonid fish sperm and their localization along sperm flagella." J.Cell Sci.104. 907-915 (1993)
[Publications] Mohri,H.: "Conformational changes of dynein molecule" In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.76. 413-416 (1992)
[Publications] Ishijima,S.: "Separation of X- and Y-chromosome-bearing sperm by free-flow electrophoresis:Evaluation of separation using PCR." Zool.Sci.9. 601-606 (1992)
[Publications] Ishijima,S.: "Identification of X- and Y-chromosome-bearing sperm separated by free-flow elecrophoresis using chromosome-specific polymerase chain reaction." Biomed.Res.13. 221-224 (1992)
[Publications] Ishida,K.: "Microflashing analysis of sperm motility." J.Reprod.Dev.39. 7-12 (1993)
[Publications] Si,Y.: "Multiple activation of mouse sperm motility." Mol.Reprod.Deveop.36. 89-95 (1993)
[Publications] Si,Y.: "The sliding of fibrous sheath through the axoneme proximally together with microtubule extrusion." Exp.Cell Res.208. 170-174 (1993)
[Publications] Nakaoka,Y.: "Hyperpolarization-activated inward current associated with the frequency increase in ciliary beating of Paramecium." J.Comp.Physiol.A. 170. 723-727 (1992)
[Publications] Nakaoka,Y.: "Cationic inhibition of Ca current and Ca-dependent ciliary responses in Paramecium." J.Comp.Physiol.A. (印刷中). (1994)
[Publications] Okamoto,K.: "Reconstitution of the metachronal waves in ciliated cortical sheet of Paramecium;I.Wave stabilities." J.Exp.Biol.(印刷中). (1994)
[Publications] Okamoto,K.: "Reconstitution of the metachronal waves in ciliated cortical sheet of Paramecium;II.Asymmetry of the ciliary movements." J.Exp.Biol.(印刷中). (1994)
[Publications] Noguchi,M.: "Effects of proteolytic digestion on the control mechanism of ciliary orientation in ciliated sheets from Paramecium." Zool.Sci.10. 267-273 (1993)
[Publications] Mogami,Y.: "Beating cilia of sea urchin embryos:Acritical comparison of the normal and reversed beating of cilia of isolated cells." J.Exp.Biol.175. 251-266 (1993)
[Publications] Mogami,Y.: "Regulation of ciliary movement in sea urchin embryos:Dopamine and 5-HT change the swimming behaviour." Comp.Biochem.Physiol.101C. 251-254 (1992)
[Publications] Ooya,M.: "Gravity-induced changes in propulsion of Paramecium:A possible role of gravireception in Protozoan behaviour." J.Exp.Biol.163. 153-167 (1992)
[Publications] Baba,S.A.: "High resolution analysis of flagellar bends." In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.75. 333-336 (1992)
[Publications] Toyoshima,Y.Y.: "Plenum Publishing Co.(eds.Sugi,H.and Pollack,G.H.)" How are myosin fragments bound to nitrocellulose film?In“Mechanism of myofilament sliding in muscle contraction"., 870(7) (1993)
[Publications] 毛利秀雄: "精子学" 東京大学出版会, 350 (1992)
[Publications] 神谷律: "細胞の運動" 培風館, 182 (1992)

1993 Fiscal Year Annual Research Report

鞭毛・繊毛運動を制御する分子調節機構の解明

Principal Investigator

森沢 正昭 東京大学, 理学部, 教授 (40013594)

Research Products

[Publications] Morisawa,M.: "Regulatory mechanisms of sperm motility." In Biology of the germ line.In animals and man.(H.Mohri,M.Takahashi,and C.Tachi,eds.). 81-93 (1993)

[Publications] Morisawa,S.: "Roles of pH and cyclic adenosine monophosphate in the aquisition of potential for sperm motilityduring migration from the river in chum salmon." Mol.Rep.Dev.34. 420-460 (1993)

[Publications] Oda,S.: "Rises of intracelular Ca^<2+> and pH mediate the initiation of sperm motility by hyperosmolality in marine teleosts." Cell Motil.Cytoskeleton.25. 497-506 (1993)

[Publications] Yoshida.M.: "Sperm chemotaxix during the process of fertilization in the ascidians Ciona savygni and Ciona intestinalis." Dev.Biol.157. 497-506 (1993)

[Publications] Yamada,A.: "Sudden increase in speed of an filament moving on myosin cross-bridges of“mismatched"polarity observed when its leading end begins to interact with cross-bgidges of“matched"polarity." J.Biochem.111. 676-680 (1992)

[Publications] Murakami,A.: "Analysis of gravitational responce of Paramecium by free-all experiments." Proc.of the 9th Space Utilization Symp.102-104 (1992)

[Publications] Murakami,A.: "Analysis of gravitation and response to microgravity in Paramecium." Proc.of the 10th Space Utilization Symp.86-87 (1993)

[Publications] 高橋景一: "鞭毛運動" Annual Review細胞生物学1993. 165-178 (1993)

[Publications] Eshel,D.: "The phase of sperm flagellar beating is not conserved over a brief imposed interruption." Exp.Cell Res.202. 552-555 (1992)

[Publications] Ogawa,K.: "Primary structure and function of a dynein motor molecule." Zool.Sci.9. 265-274 (1992)

[Publications] 小川和男: "鞭毛モーターダイニンの分子構造" Cell Science. 9. 33-41 (1993)

[Publications] Ogawa,K.: "cDNA cloning for mouse caltractin" Biochim.Biophys.Acta.1216. 126-128 (1993)

[Publications] Kamimura,S.: "High-frequency vibration in flagellar axonemes with amplitudes reflecting the size of tubulin." J.Cell Biol.116. 1443-1454 (1992)

[Publications] Kamiya,R.: "Molecular mechanism of ciliary and flagellar movement" Advances in Comparative and Enviromental Physiology. 12. 206-226 (1992)

[Publications] Takada,S.: "Three-headed outer arm dynein from Chlamydomonas that can functionally combine with outer arm-missing axonemes." J.Biochem.111. 758-762 (1992)

[Publications] Kagami,O.: "Translocation and lotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein." J.Cell Biol.103. 653-664 (1992)

[Publications] Sakakibara,H.: "A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain." J.Cell Biol.122. 653-662 (1993)

[Publications] Kato,T.: "Isolation of two species of Chlamydomonas reinhardtii flagellar mutants,ida5 and ida6,that lack a newly identified heavy chain of the inner dynein arm" Cell Structure and Function. (印刷中). (1994)

[Publications] Johara,M.: "Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion" Proc.Natl.Acad.Sci.USA.90. 2127-2131 (1993)

[Publications] Itakura,S.: "Force-generating domain of myosin motor." Biochem.Biophys.Res.Commun.196. 1504-1510 (1993)

[Publications] 豊島陽子: "in vitro運動系" Annual Review細胞生物学1993. 3. 145-155 (1993)

[Publications] Iwamoto,T.: "Purification and characterization of a sperm motility-dynein ATPase inhibitor from boar seminal plasma." Mol.Reprod.Deveop.31. 55-62 (1992)

[Publications] Wada,S.: "Dynein of sperm flagella of oyster belonging to Protostomia also has a two headed structure" Biol.Cell. 76. 311-317 (1992)

[Publications] Mohri,H.: "Role of tubulin and dynein in spermatozoan motility" Mol.Reprod.Develop.36. 221-223 (1993)

[Publications] 高谷知恵子: "電気泳動法を応用したタンパク質の分離精製" 比較生理生化学. 9. 156-159 (1992)

[Publications] Wazawa,T.-I.: "Hydrogen peroxide plays a key role in the oxidation reaction of myoglobin by molecular oxygen." Biophys.J.63. 544-555 (1992)

[Publications] Jin,Z.X.: "Isolation and properties of a protein complex that cantains flagellar movement initiating-phosphoprotein from testis of chum salmon." J.Biochem.(印刷中). (1994)

[Publications] Jin,Z.X.: "Monoclonal antibodies against the protein complex that contains the flagellar movement initiating-phosphoprotein of Onchorhynchus keta." J.Biochem.(印刷中). (1994)

[Publications] 森沢正昭: "特集を終えるにあたって" Cell Science. 9. 1993 (1992)

[Publications] Inaba,K.: "Two high molecular mass proteases from sea urchin sperm" Biochem.Biophys.Res.Commun.182. 667-674 (1992)

[Publications] Inaba,K.: "Chymotrypsin-like protease activity associated with demembranated chum salmon sperm." Biol.Cell. 76. 329-333 (1992)

[Publications] 稲葉一男: "精子のプロテアソーム" Cell Science. 8. 750-758 (1992)

[Publications] Inaba,K.: "Interactions among the heterogenous components of dynein" In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.76. 385-386 (1992)

[Publications] Inaba,K.: "Purification of fproteasomes from salmonid fish sperm and their localization along sperm flagella." J.Cell Sci.104. 907-915 (1993)

[Publications] Mohri,H.: "Conformational changes of dynein molecule" In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.76. 413-416 (1992)

[Publications] Ishijima,S.: "Separation of X- and Y-chromosome-bearing sperm by free-flow electrophoresis:Evaluation of separation using PCR." Zool.Sci.9. 601-606 (1992)

[Publications] Ishijima,S.: "Identification of X- and Y-chromosome-bearing sperm separated by free-flow elecrophoresis using chromosome-specific polymerase chain reaction." Biomed.Res.13. 221-224 (1992)

[Publications] Ishida,K.: "Microflashing analysis of sperm motility." J.Reprod.Dev.39. 7-12 (1993)

[Publications] Si,Y.: "Multiple activation of mouse sperm motility." Mol.Reprod.Deveop.36. 89-95 (1993)

[Publications] Si,Y.: "The sliding of fibrous sheath through the axoneme proximally together with microtubule extrusion." Exp.Cell Res.208. 170-174 (1993)

[Publications] Nakaoka,Y.: "Hyperpolarization-activated inward current associated with the frequency increase in ciliary beating of Paramecium." J.Comp.Physiol.A. 170. 723-727 (1992)

[Publications] Nakaoka,Y.: "Cationic inhibition of Ca current and Ca-dependent ciliary responses in Paramecium." J.Comp.Physiol.A. (印刷中). (1994)

[Publications] Okamoto,K.: "Reconstitution of the metachronal waves in ciliated cortical sheet of Paramecium;I.Wave stabilities." J.Exp.Biol.(印刷中). (1994)

[Publications] Okamoto,K.: "Reconstitution of the metachronal waves in ciliated cortical sheet of Paramecium;II.Asymmetry of the ciliary movements." J.Exp.Biol.(印刷中). (1994)

[Publications] Noguchi,M.: "Effects of proteolytic digestion on the control mechanism of ciliary orientation in ciliated sheets from Paramecium." Zool.Sci.10. 267-273 (1993)

[Publications] Mogami,Y.: "Beating cilia of sea urchin embryos:Acritical comparison of the normal and reversed beating of cilia of isolated cells." J.Exp.Biol.175. 251-266 (1993)

[Publications] Mogami,Y.: "Regulation of ciliary movement in sea urchin embryos:Dopamine and 5-HT change the swimming behaviour." Comp.Biochem.Physiol.101C. 251-254 (1992)

[Publications] Ooya,M.: "Gravity-induced changes in propulsion of Paramecium:A possible role of gravireception in Protozoan behaviour." J.Exp.Biol.163. 153-167 (1992)

[Publications] Baba,S.A.: "High resolution analysis of flagellar bends." In Comparative Spermatology 20 Years After(Baccetti,B.,ed.)Raven Press,New York.75. 333-336 (1992)

[Publications] Toyoshima,Y.Y.: "Plenum Publishing Co.(eds.Sugi,H.and Pollack,G.H.)" How are myosin fragments bound to nitrocellulose film?In“Mechanism of myofilament sliding in muscle contraction"., 870(7) (1993)

[Publications] 毛利秀雄: "精子学" 東京大学出版会, 350 (1992)

[Publications] 神谷律: "細胞の運動" 培風館, 182 (1992)

森沢正昭東京大学, 理学部, 教授 (40013594)