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1995 Fiscal Year Final Research Report Summary

Evolution of pituitary hormones

Research Project

Project/Area Number 04404005
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionWaseda University

Principal Investigator

ISHII Susumu  Waseda Unv., Sch.Edu.Professor, 教育学部, 教授 (90063528)

Co-Investigator(Kenkyū-buntansha) ISHIGAKI Haruo  Waseda Univ., Sch.Edu.Professor, 教育学部, 教授 (60063492)
KUBOKAWA Kaoru  Univ.Tokyo, Ocea.Res.Inst.Research Associate, 海洋研究所, 助手 (30240740)
KIKUCHI Motoshi  Waseda Univ., Hum.Sci.Cent.Research Fellow, 人間総合研究センター, 研究員
WAKO Hiroshi  Waseda Univ.Sch.Soc.Sci.Professor, 社会科学部, 教授 (60158607)
KIKUYAMA Sakae  Waseda univ., Sch.Edu.Professor, 教育学部, 教授 (20063638)
Project Period (FY) 1992 – 1995
Keywordsgonadotropin / luteinizing hormone / follicle-stimulating hormone / beta-subunit / alpha-subunit / phylogenic tree / molecular evolution / secondary structure
Research Abstract

We have cloned cDNAs encoding precursor molecules of luteinizing hormone (LH) beta subunits of the chicken and Japanese quail, the beta subunit of follicle-stimulating hormone of Japanese quail and alpha subunits of the Japanese quail, bullfrog, toad and Australian lung-fish. Using these data and data of other vertebrate species reported, we compared the primary structure of the molecules among vertebrate species. The primary structure of the alpha subunits was highly conserved and that of the beta subunits was less conserved. We also predicted the secondary sturctures of the alpha and beta subunits of these gonadotropins and thyrotropins. It was very well coincided among vertebrate species in both subunits. We concluded that the three dimensional structure is more highly conserved than the primary structure in vertebrates. We constructed a phylogenic tree of the beta subunits of gonadotropins and thyrotropin. It was found that follicle-stimulating hormone is more closely related to thyrotropin than luteinizing hormone. This is different from the classic grouping of members of the gonadotropin/thyrotropin family in which follicle-stimulating hormone and luteinizing hormone used to be categorized together as gonadotropin and thyrotropin was separated from gonadotropin. We also found that the alpha subunit of Australian lung-fish is more closely related to alpha subunits of tetrapod vertebrates than to those of teleosts. A similar result was obtained in neurohypophyseal hormone precursor molecules.

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Kawashima,S.,K. Tsutsui,R. N. Saxena and S. Ishii: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and plasma gonadotropin concentrations in Indian Weaver Birds" Gen. Comp. Endocrimol.92. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taguchi,N. and S. Ishii: "Effect of long-days on the expression of gonadotropin subunit genes in Japanese quail." Zoological Science. 10. 132-132 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S. 石居進: "ホルモンの種特異性と分子構造" 遺伝. 1993. 69-75 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S.: "生殖腺刺激ホルモンの化学構造とその利用" 遺伝. 1993. 8-14 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawai,K., . C. Wingfield,and S. Ishii: "Changes in blood sugar of wild Japanese toads(Bufojaponicus)during the breeding migration. (Abstract)" Zoologica Science. 10. 170-170 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ando,H. and S. Ishii: "Molecular cloning of complimentary deoxiribonucleic acids for the pituitary glycoprotein hormone α-subunit and luteinizing hormone β-subunit precursor molecules of Japanese quail(Coturnix coturnix japonica)." Gen. Comp. Endocrinology. 93. 357-368 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mauget,Robert,Pierre Jouventin,Andre Lacroix and Susumu Ishii: "Plasma LH and steroid hormones in King Penguin(Aptenodytes patagonicus)duing the onset of the breeding cycle" Gen. Comp. Endocrinol.93. 36-43 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S.,M. Wada,S. Wakabayashi,H. Sakai,Y. Kubodera,N. Yamaguchi: "Endocrinological studies for artificail breeding of the Japanese ibis,Nipponia nippon,an endangered avian species in Asia" J. Biosci.19. 491-502 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsutsui,K.,S. Kawashima,R. N. Saxena and S. Ishii: "Effect of season and photoperiod on the follicle-stimulating hormone receptors in a subtropical bird" J. Biosci.19. 485-489 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S.: "Evolution of gonadotropin" Hormone Frontier in Gynecology. 1. 211-218 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 石居進: "ヒキガエルの繁殖期の行動" 動物と動物園. 46. 288-291 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubokawa,K. S. Ishii and J. C. Wingfield: "Effect of day length on luteinizing hormone β-subunit mRNA and subsequnet gonadal growth in the white-crowned sparrow,Zonotrichia leucophrys gambelii." Gen. Comp. Endocrinol.95. 42-51 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ishii,Y. Miya and H. Wako: "Why chicken luteinizing hormone (LH) is recognized as follicle-stimulating hormone (FSH) by mammalian gonadal receptors : A molecular approach (Abstract)" Zool. Sci.11(s). 7-7

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Ishii and Y. Ooka: "Effects of stress and female sex steroid hormones on the release vibration of Japanese toads,Bufo japonicus. (Abstract)" Zool. Sci.11(s). 14-14 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakazawa,H.,K. Koh,W. Takahashi,S.Ishii and S. Kaji: "Changes in the electroolfactogram with season and stages of the breeding migration in the Japanese toad,Bufo japonicus. (Abstract)" Zool. Sci.11(s). 15-15 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Itoh,A. and S. Ishii: "Disappearance of temperature dependency of affinity in FSH receptors of the Japanese quail by solubulization. (Abstract)" Proc. Japan Soc. Comp. Endocr.No. 9. 56-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miya,Y.,H. Wako and S. Ishii: "Rat FSH receptor recognizes chicken LH as FSH(Abstract)" Proc. Japan Soc. Comp.Endocr.No. 9. 57-57 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wako,H. and S. Ishii: "Secondary structure prediction of β-subunits of the gonadotropin-thyrotropin family from its aligned sequences using environment-dependent amino-acid and conformational propensities" Biochim. Biophys. Acta. 1247. 104-112 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S.,K. Kubokawa,M. Kikuchi and H. Nishio: "Orientation of toad,Bufo japanicus,toward the breeding pond" Zool. Sci.12. 463-472 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wilson,J. X.,H. Sawai,M. Kikuchi,K. Kubokawa and S.Ishii: "Circulating cathecolamine and glucose concentrations in Japanese toads(Bufo japonicus)during the breeding season" Gen. Comp. Endocrinol.98. 303-310 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wingfild,J. C.,K. Kubokawa,k. Ishida,S. Ishii and M.Wada: "The adrenocortical responese to stress in male bush warblers,Cettia diphone : a comparison of breeding popultions in Honshu and Hokkaido,Japan" Zool.Sci.12. 615-621 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyoshi Tsusui,Mariko Usui,Takeru Kotegawa,Yasuo Furukawa and Susumu Ishii: "Regulatory mechanism of the avian reproductive behavior" J. Yamashina Inst. Ornithol.27. 47-65 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,S.: "The Molecular Biology of Avian Gonadotropin" Poultry Sci.72. 856-866 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuchi,M,K.Sawai,J.X.Wilson and S.Ishii: "Effect of stress on plasma glucose level in the toad,Bufo Japonicus(Abstract)" Zoological Science. 10. 123-134 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii s.,H.Ando,H.Wako and Y.Kubota: "Avian Molecular Biology of Gonnadotrophins Endocrinology" The Endocrinology Society U.K., 123-134 (1993)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 1997-03-04  

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