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1994 Fiscal Year Final Research Report Summary

Paracrine regulation of reproductive system by TGFbeta superfamily

Research Project

Project/Area Number 04404018
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 基礎獣医学
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAHASHI Michio  The Univ.of Tokyo, Fac.Agriculture, Professor, 農学部, 教授 (30011943)

Co-Investigator(Kenkyū-buntansha) MATSUYAMA Shigemi  The Univ.of Tokyo, Fac.Agriculture, Assistant Professor, 農学部, 助手 (80219526)
NISHIHARA Masugi  The Univ.of Tokyo, Fac.Agriculture, Associate Professor, 農学部, 助教授 (90145673)
SHIOTA Kunio  The Univ.of Tokyo, Fac.Agriculture, Associate Professor, 農学部, 助教授 (80196352)
TACHI Chikashi  The Univ.of Tokyo, Fac.Agriculture, Professor, 農学部, 教授 (30011711)
HAYASHI Yoshihiro  The Univ.of Tokyo, Fac.Agriculture, Professor, 農学部, 教授 (90092303)
Project Period (FY) 1992 – 1994
KeywordsTGF-beta / Activin / Inhibin / Follistatin / Ovary / Embryogenesis / Pituitary / Immuno assay
Research Abstract

Recently, the evidence has been accumulated that transforming growth factor- (TGF) beta superfamily growth factors play indispensable roles for differentiation, cell proliferation or expression of the function of various tissues. In this research project, we focused on the physiological roles of TGFbeta superfamily growth factors and related peptides in reproductive system. The summary of the results are as follows :
1) We found that activin release "2-cell block" during early embryogenesis and enhance embtyo development in mice. Immunohistochemical analysis and in situ hybridization technique to detect mRNA revealed that activin is synthesized both in the embryo and the oviduct. Activin receptor mRNA was detected in embryos but not in oviductal cells. These results suggest that activin is an autocrine factor to promote embryo development and oviductal cells help its growth by supplying additional activin.
2) The mode of action of activin to regulate gonadotropin-secreting-cells in the p … More ituitary was investigated. Activin increased the number of follicle stimulating hormone secreting-cells specifically. Furthermore, it was found that folliculo-stelate cells in the pituitary secrete follistatin and this peptide regulate activin's paracrine/autocrine action by inhibiting its bioactivity.
3) TGFbeta was found to be a paracrine factor from macrophage to mediate prolactin action maintaining luteal progesterone secretion in rats. We also succeeded in isolating and characterizing the cDNA of 20alpha-hydroxysteroid dehydrogenase, which is one of the target proteins which expression are regulated by TGFbeta in the ovary.
4) We succeeded in producing specific antibodies to activin/inhibin betaA and betaB subunits, inhibin alpha subunits, and follistain. Using these antibodies we established immunoassay system for these bioactive polypeptides.
5) We Succeeded in isolating cDNA clone of equine avtivin/inhibin betaA subunit. Cloning of equine betaB or alpha subunits, follistatin is also now under way. Less

  • Research Products

    (70 results)

All Other

All Publications (70 results)

  • [Publications] Seong,H.et al.: "Expression of activities of two 20α-hydroxysteroid dehydrogenase isozymes in rat corpora lutea." J.Reprod.Ferti.96. 573-580 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda,K.et al.: "Changes in two kind of 20α-hydroxysteroid dehydrogenase during the luteal phase in the rat." J.Reprod.Dev.38. 49-53 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama,S.et al.: "Splenic Macrophage enhancce luteotropic action of prolactin and luteinizing hormone in rat luteal cell cultures." Endocrinol.Jpn.39. 51-57 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lu,R.-Z.et al.: "Histochemical demonstration of activin/inhibin βA-,βB-and α-subunits in the early embryos and oviducts of different strains of mice." J.Reprod.Dev.38. 79-90 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,S.et al.: "Changes in ratios of phagocytotic macrophages in the splenic adherent cells during estrous cycle and pseudopregnancy in rat." J.Reprod.Dev.38. 115-120 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katayama et al.: "Paracrine effect of folliculo-stellate cells on the growth factor-like action of activin A in anterior pituitary cultutes." Endocrinol.Jpn.39. 289-297 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamanouchi,K.et al.: "Splenic macrophages enhance prolactin-induced progestin secretion from mature rat granulosa cells in vitro." Biol.Reprod.46. 1109-1113 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,S.et al.: "Translation of rat ovarian mRNA to two 20α-hydroxysteroid dehydrogenase isozymes in Xenopus oocytes." Endocrinol.Jpn.39. 365-369 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miura,R.et al.: "Changes in 20α-hydroxysteroid dehydrogenase mRNA in the ovary of immature rats bearing a single generation of corpora lutea." J.Reprod.Dev.38. 249-254 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa K et al.: "Immunoreactive βA subunit of inhibin/activin is present in cytoplasma of rat spermatogenec cells." J.Reprod Dev. 38. 5-9 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamei,T et al.: "Basic fibroblast growth factor-like substance associates with DNA in germ cell nuclei." J.Reprod.Dev.39. 25-31 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamei,T.et al.: "Expression of basic frowth factor-like substance in immature rat testis:Immunocytochemical localization and detection of mRNA." J.Reprod.Dev.39. 33-39 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda,K.et al.: "Rat ovarian 20α-hydroxysteroid dehydrogenase-1 may belong to aldo-keto reductase superfamily." J.Reprod.Dev.39. 169-173 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lu,R.-Z.et al.: "Developmental expression of activin/inhibin βA,βB and α subunits and acctivin receptor genes in preimplantation mouse embrryos." Biol.Reprod.49. 1163-1169 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiota,K.et al.: "20α-Hydroxysteroid dehydrogenase activity in rat placenta." Endocrine J.40. 673-681 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamanouchi,K.et al.: "Effect of calyculin-A,phosphatase inhibitor,on 20α-hydroxysteroid dehydrogenase activity in rat luteal cell culture." Endocrine J.40. 699-704 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hattori,H.et al.: "Charaterization of Rat Placental Lactogen-a(PL-a)with an Antipeptide Antibody Directed against Rat PLs." Endocrine J.40. 727-735 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa,K.et al.: "Expression of α,βA and βB subunits of inhibin or activin and follistatin in rat pancreatic islets." FEBS. 319. 217-220 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiota,K.et al.: "Expression of Placental lactogen(s)and 20α-hydroxysteroid dehydrogenase in the rat placenta." Proc.J.Soc.Anim.Biochem.30. 1-6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miura,R.et al.: "Molecular cloning of cDNA for rat ovarian 20α-hydroxysteroid dehydrogenase (HSD1)." Biochem.J.299. 561-567 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka,N-M.et al.: "20α-hydroxysteroid dehydrogenase in canine spontaneous neoplasm" J.Vet.Med.Sci.56. 581-583 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lu,R-Z.et al.: "Activin A and superoxide dismutase synergistically enhance development of 1-cell mouse embryos to the blastocyst stage in vitro." J.Reprod.Dev.40. 279-284 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirosawa M et al.: "A cDNA encoding a new member of the rat placental lactogen family,PL-1 mosaic(PL-1m)." Endocrine J.41. 387-397 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shiota K et al.: "Alteration of basal release of anterior pituitary hormones by pretreatment of primary cultured Cells with trypsin." Molecular and Cellular Endocrinology. 101. 315-320 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa K et al.: "Changes in immunoreactivity of follistatin within preovulatory follicles after primary gonadotropin surge in rat." J Reprod Fert. 101. 577-581 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama S et al.: "Immunoreactive(ir)-Transforming Growth Factor(TGF)-β in Rat Corpus Luteum:ir-TGFβ is Expressed by Luteal Macrophages." Endocrine J.42. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida S et al.: "Molecular cloning of cDNA for equine ovarian inhibin/activin βA subunit." J Vet Med Sci. 57. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa K et al.: "Effect of streptozotocin injection on expression of immunoreactive follistatin and βA and βB subunits of inhibin/activin in rat pancreatic islets." Eur J Endocrinol. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松山茂実 他: "卵巣の機能発現に必須な免疫系細胞の役割。" 医学のあゆみ. 160. 219-221 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋迪雄 他: "アクチビンとフォリスタチンによるFSH分泌の調節." 細胞. 24. 78-83 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 呂容真 他: "胚発生と細胞成長因子-マウス培養受精卵に対するアクチビンの効果-" J.Reprod.Dev.38. 2147-2155 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋迪雄 他: "培養マウス受精卵に対するアクチビンの効果." アニテックス. 4. 83-88 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋迪雄: "バイオテクノロジーの動物生産への適用." 遺伝. 47. 41-45 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松山茂実 他: "ラット黄体退行をモデルとしたcDNAサブトラクション法によるプログラム細胞死最初期遺伝子の探索." 獣医畜産新報. 48. 117-118 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takahashi,M.et al.: "In:Biology of the Germ Line in Animals and Man(eds H.Mohri,H.,Takahashi,M.and Tachi,C.)Japan" Scientific Society Press and Karger, 304 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seong, H.et al.: "Expression of activities of two 20a-hydroxysteroid dehydrogenase isozymes in rat corpora lutea." J.Reprod.Ferti.96. 573-580 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, K.et al.: "Changes in two kind of 20a-hydroxysteroid dehydrogenase during the luteal phase in the rat." J.Reprod.Dev.38. 49-53 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, S.et al.: "Splenic Macrophage enhancce luteotropic action of prolactin and luteinizing hormone in rat luteal cell cultures." Endocrinol.Jpn.39. 51-57 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lu, R.-Z.et al.: "Histochemical demonstration of activin/inhibin betaA-, betaB-and alpha-subunits in the early embryos and oviducts of different strains of mice." J.Reprod.Dev.38. 79-90 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, S.et al.: "Changes in ratios of phagocytotic macrophages in the splenic adherent cells during estrous cycle and pseudopregnancy in rat." J.Reprod.Dev.38. 115-120 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama et al.: "Paracrine effect of folliculo-stellate cells on the growth factor-like action of activin A in anterior pituitary cultures." Endocrinol.Jpn.39. 289-297 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamanouchi, K.et al.: "Splenic macrophages enhance prolactin-induced progestin secretion from mature rat granulosa cells in vitro." Biol.Reprod.46. 1109-1113 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, S.et al.: "Translation of rat ovarian mRNA to two 20alpha-hydroxysteroid dehydrogenase isozymes in Xenopus oocytes." Endocrinol.Jpn.39. 365-369 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura, R.et al.: "Changes in 20a-hydroxysteroid dehydrogenase mRNA in the ovary of immature rats bearing a single generation of corpora lutea." Endocrinol.Jpn.39. 365-369 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa K et al.: "Immunoreactive bA subunit of inhibin/activin is present in cytoplasma of rat spematogenec cells." J.Reprod Dev. 38. 5-9 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamei, T et al.: "Basic fibroblast growth factor-like substance associates with DNA in germ cell nuclei." J.Rerod.Dev.39. 25-31 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamei, T.et al.: "Expression of basic frowth factor-like substance in immature rat testis : Immunocytochemical localization and detection of mRNA." J.Reprod.Dev.39. 33-39 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, K.et al.: "Rat ovarian 20alpha-hydroxysteroid dehydrogenase-1 may belong to aldo-keto reductase superfamily." J.Reprod.Dev.39. 169-173 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lu, R.-Z.et al.: "Developmental expression of activin/inhibin betaA,betaB and alpha subunits and acctivin receptor genes in preimplantation mouse embrryos." Bio.Reprod.49. 1163-1169 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiota, K.et al.: "20alpha-Hydroxysteroid dehydrogenase activity in rat placenta." Endocrine J.40. 673-681 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamanouchi, K.et al.: "Effect of calyculin-A,phosphatase inhibitor, on 20alpha-hydroxysteroid dehydrogenase activity in rat luteal cell culture." Endocrine J.40. 699-704 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hattori, H.et al.: "Charaterization of rat placental lactogen-alpha (PL-alpha) with an antipeptide antibody derected against rat PLs." Endocrine J.40. 727-735 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa, K.et al.: "Expression of alpha, betaA andbetaB subunits of inhibin or activin and follistatin in rat pancreatic islets." FEBS. 319. 217-220 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiota, K.et al: "Expression of Placental lactogen (s) and 20alpha-hydroxysteroid dehydrogenase in the rat placenta." Proc.J.Soc.Anim.Biochem.30. 1-6 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miura, R.et al.: "Molecular cloning of cDNA for rat ovarian 20alpha-hydroxysteroid dehydrogenase (HSD1)" Biochem.J.299. 561-567 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, N-M.et al.: "20alpha-hydroxysteroid dehydrogenase in canine spontaneous neoplasm" J.Vet.Med.Sci.56. 581-583 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lu, R-Z.et al.: "Activin A and speroxide dismutase synergistically enhance development of 1-cell mouse embryos to the blastocyst stage in vitro." J.Reprod.Dev.40. 279-284 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirosawa M et al.: "A cDNA encoding a new member of the rat placental lactogen family, PL-1 mosaic (PL-1m)" Endocrine J.41. 387-397 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiota K et al.: "Alteration of basal release of anterior pituitary hormones by pretreatment of primary cultured cells with trypsin." Molecular and Cellular Endocrinology. 101. 315-320 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa K et al.: "Changes in immunoreactivity of follistatin within preovulatory follicles after primary gonadotropin surge in rat." J Reprod Fert. 101. 577-581 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama S et al.: "Immunoreactive (ir) -Transforming Growth Factor (TGF) -beta in Rat Corpus Luteum : ir-TGFbeta is Expressed by Luteal Macrophages." Endocrine J.42 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida S et al.: "Molecular cloning of cDNA for equine ovarian inhibin/activin betaA subunit." J Vet Med Sci. 57 (3) (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa K et al.: "Effect of streptozotocin injection on expression of immunoreactive follistatin and betaA and betaB subunits of inhibin/activin in rat pancreatic islets." Eur J Endocrinol. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama S et al.: "An essential role of immunocytes for the expression of ovarian function (in Japanese)" Progress in Medical Science (Igaku no Ayumi). 160. 219-221 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi M et al: "Regulation of FSH secretion by activin and follistatin. (in Japanese)" Cell (Saibou). 24. 78-83 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lu et al: "Embryonal development and growth factors : Effect of activin on mouse fertilized ova in vitro. (in Japanese)" J Reprod Dev. 38. j147-j155 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi M et al.: "Effect of activin on mouse early embryos in culture (in Japanese)" Anitex. 4. 83-88 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi M et al.: "Biotechnology ; application to animal production. (in Japanese)" Genetics (Iden). 47. 41-45 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama S et al.: "Isolation of programd cell death genes from rat corpora lutea by cDNA subtraction" J Vet Med. 48. 117-118 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takahashi, M.et al: "Paracrine/autocrine regulation in thte murine reproductive process. In : Biology of the Germ Line in Animals and Man (eds H.Mohri, H., Takahashi, M.and Tachi, C.) Japan" Scientific Society Press and Karger. 63-80

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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