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1993 Fiscal Year Final Research Report Summary

Histochemical and Immunohistochemical Studies on the Mechanisms of Calcium Regulation by Dental and Periodontal Hard Tissue-Forming Cells (1993)

Research Project

Project/Area Number 04404069
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

TAKANO Yoshiro  Niigata University, Department of Oral Anatomy II, Professor, 歯学部, 教授 (90126425)

Co-Investigator(Kenkyū-buntansha) OHSHIMA Hayato  Niigata University, Department of Oral Anatomy II, Research Associate, 歯学部, 助手 (70251824)
MAEDA Takeyasu  Niigata University, Department of Oral Anatomy II Associate Professor, 歯学部, 助教授 (40183941)
Project Period (FY) 1992 – 1993
KeywordsCalcium / GBHA / Histochemistry / X-ray microanalysis / Ameloblasts / Enamel / Periodontal tissue / Mineralization
Research Abstract

In order to elucidate the mechanisms whereby cells of the dental and periodontal mineralized tissue-forming cells regulate calcium, our research group introduced a new method to reveal possible Ca-binding sites in cell layrs by vascularly perfusing animals with a high Ca-containing solution followed by anhydrous tissue processing for light and electron microscopy and X-ray microanalysis. With this method we found granular precipitates of Ca-phosphate to occur exclusively in the ameloblasts at the stage of matrix formation, and the smooth-ended maturation ameloblasts (SA). A complete absence of such precipitate in bone-related cells was also noted.
In the second research term, we examined whether or not the peculiar phenomenon is specific for continuously growing teeth or is an essential one for mammalian amelogenesis. In the molar tooth germs of high-Ca perfused young rats, we observed similar mineral deposits in the ameloblast layr as those in the incisors. The inhibition of two types of Ca-binding proteins having been assumed to be related to cellular Ca-transport, and known to be contained in the cytoplasm of ameloblasts, did not make any changes in mineral deposits in the ameloblast layr.
The presence of putative Ca-binding sites that appear to bind with excessively high concentration of calcium entering in the cytoplasm has been shown to be a common feature of the amelogenesis at least in rat teech, implicated its relation to the yet unknown mechamism of calcium regulation by the ameloblasts.
We would like to further elaborate the biological significance of peculiar mineral deposits in Ca-loaded ameloblasts in future studies.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] YOSHIRO TAKANO: "GBHA staining method:Its application to demonstration of cytoplasmic calcium stores in some exoerine and endocrine glands." Hard Tissue Biology. (99)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YOSHIRO TAKANO: "Histochemical localization and X-ray microanalysis of Calcium in the rat submandibular gland:Demonstration of possible sites for microlith formation." Arch.Histol.Cytol.56. 177-184 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] AKIRA JIKKO: "Characterization of the mineralizing process in cultures of rabbit growth plate chondrocytes." Development.Biol.193. 327-380 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YOSHIRO TAKANO: "Histochemical demonstration of calcium phosphate depositions in the enamel organ of calcium-loaded tooth germs of the rat." Arch.Comp.Biol.Tooth Enamel. 3. 57-64 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YOSHIRO TAKANO: "Histochemical aspects of calcium regulation by the enamel forming cells during matrix formation and maturation." Acta Anat.Nipponica. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高野吉郎: "歯根端部の解剖" 歯科ジャーナル. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YOSHIRO TAKANO: "Calcium movement in vivo and in vitro in secretory stage enamel of rat incisors." Archs Oral Biol.37. 377-383 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ICHIRO KAWAHARA: "Histochemical and immunohestochemical demonstration of macrophages and dendritic cells in the lingual periodontal ligament of rat incisors." Arch.Histol.Cytol.55. 211-217 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] YOSHIRO TAKANO: "Histochemical demonstration and microanalysis of possible calcium binding sites in the enamel organ of rat incisors." Scanning Microscopy. 6. 773-784 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高野吉郎: "歯牙発生とエナメル質形成" 病態生理. 11. 830-838 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高野吉郎: "組織細胞化学の技術・無機質と色素" 朝倉書店(印刷中), (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Takano, M.A.Crenshaw and J.W.Bawden: "Calcium movement in vivo and in vitro in secretory stage enamel of rat incisors." Archs Oral Biol.37. 377-383 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Kawahara, Y.Takano, O.Sato, T.Maeda and K.Kannari: "Histochemical and immunohistochemical demonstration of macrophages and dendritic cells in the lingual periodontal ligament of rat incisors." Arch.Histol.Cytol. 55. 211-217 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takano: "Histochemical demonstration and microanalysis of possible calcium binding sites in the enamel organ of rat incisors." Scanning Microscopy. 6. 773-784 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Jikko, T.Aoba, H.Murakami, Y.Takano, M.Iwamoto and Y.Kato: "Characterization of the mineralizing process in cultures of rabbit growth plate chondrocytes." Developmental Biol.156. 372-380 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takano, H.Oshima, T.Maeda and Y.Sato: "GBHA staining method : Its application to demonstration of cytoplasmic calcium stores in some exocrine and endocrine glands." Hard Tissue Biology.2. 20-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takano, Y.Sato, H.Ohshima, T.Maeda, I.Kawahara and I.Noguchi: "Histochemical localization and X-ray microanalysis of calcium in the rat submandibular gland : Demonstration of possible sites for microlith induction." Arch.Histol.Cytol.56. 177-184 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takano and H.Ohshima: "Histochemical demonstration of calcium phosphate depositions in the enamel organ of calcium-loaded tooth germs of the rat." Arch.Comp.Biol.Tooth Enamel. 3. 57-64 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takano: "Histochemical aspects of calcium regulation by the enamel forming cells during matrix formation and maturation." Acta Anatomica Nipponica. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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