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1994 Fiscal Year Final Research Report Summary

Physio-chemical Analysis on the Mechanism of Conjugation between Eukyaryote and Prokaryote.

Research Project

Project/Area Number 04454015
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionHOROSHIMA UNIVERSITY

Principal Investigator

YOSHIDA Kazuo  Fac. Science, Biological Science, Hiroshima University Professor, 理学部, 教授 (70022720)

Co-Investigator(Kenkyū-buntansha) SHIDARA Sohsuke  Fac. Integeral Arts & Sci., Environmental Science, Assoc. Professor, 総合科学部, 助教授 (90034587)
SUZUKI Katsunori  Fac. Science, Biological Science, Assistant, 理学部, 助手 (50221320)
Project Period (FY) 1992 – 1994
Keywordstrans-kindom conjugation / organelles origin and evolution / conjugative plasmids / Escherichia coli / yeasts / YAC (yeast artificial chromosome) / sex / endosymbiotic conjugation hypothesis
Research Abstract

Trans-kindom conjuation between prokaryotic Escherichia coli and eukaryotic Saccharomyces cerevisiae yeast required the sexual cell aggultination in which the conjugation occurred. The cell surface substances of both bacterium and yeast involved in the agglutination. The sex plasmid which were transmitted, were generally recirculized and replicated as plasmids in transconjugants. However, at very rare frequency, the plasmids were integrated into the transconjugants chromosomes by double crossing over. The conjugation process directly involves into the integration because this type integration is very rare in transformation. Newly constucted conjugative YACs in which oriT was inserted were successfully transferred into yeast recipient from E.coli. E.coli also could conjugate with Saccharomyces kluyveri which was a relatively remote species from S.cerevisiae. This conjugation induced a high frequent plasmid recombination. Agrobacterium tumafaciens could conjugate with S.cerevisiae. We compared T-DNA transfer with conjugate transfer of Ti-plasmid in Agrobacterium. Finally, we have proposed a novel hypothesis which describes the role of trans-kingdom conjugation on the origin and evolution of mitochondrion and chloroplast.

  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] M.Yamaguchi,K.Yoshida and N.Yanagishima: "Sexual agglutination subotanos requires a carrier glycoproteins for integration into the cell wall of Saccharomyces cerevisiae." Microbiology. 140. 2217-2223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Okamoto,T.Sekito and K.Yoshida: "The mitochondrial genome of yeast Hanseruls wingei encodes NADH dehydrogenase genes ND4L and ND5." Molecular and General Genetics. 243. 473-476 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Inomata,M.Nishikawa and K.Yoshida: "The yeast Saccharomyces Rluyreri as a recipient eukaryote:Beharior of transmitted plasmids in transconjugants." Jounal of Bacteriology. 176. 4770-4773 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Sekito,K.Okamoto,H.Kitano and K.Yoshida: "Yeast Hansenula wingei mitochondrial genome's complete DNA sequene demonstrated usique characteristics." Nucleic Acids Symposium Series. 31. 233-234 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Sekito,K.Okamoto,H.Kitano and K.Yoshida: "The complete mitochondrial DNA sequence of Hansenula wingei reveals new characteristics of yeast mitochondria." Current Genetics. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yoshida,M.Nishikawa and A.Katoh20GB06:Endosymbiotic conjugation:A new hypothetical mechanism for gene transfer to the host nucleus during the organolles origin.: Viva Origino. (accepted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 吉田和夫、谷口寿章、杉浦昌弘編著: "蛋白質・核酸・酵素・増刊号(共立出版)" バイオ高性能機器導入・共同利用マニュアル, 381 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa, M., Suzuki, K.and Yoshida, K.: "DNA integration into recipient yeast chromosome by trans-kingdom conjugation between Escherichia coli and Saccharomyces cerevisiae." Curr. Genet.21. 101-108 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., Suzuki, K.and Yoshida, K.: "Nucleotide sequences of ten mitochondrial genes in yeast Hansenula wignei." Nucleic Acids Res.20. 2373-2374 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M., Inomata, K., Sekito, T., Suzuki, K.and Yoshida, K.: "Trans-kingdom conjugation as a sumbiotic plasmid transfer between bactoria and yeasts." in Endocytobilogy V (ed by Satoh, S Ishida, M.and Ishikawa, H.) p.537-544. Tubingen Unversity Press.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., Sekito, T., Suzuki, K.and Yoshida, K.: "Physical mapping , DNA sequencing and RFLP analysis of mitochondrial DNAs from ascosporogenous yeasts : Saccharomyces exiguus, Saccharomyces kluyveri and Hansenula wingei." in Endocytobilgoy V (ed by Satoh, S Ishida, M.and Ishikawa, H.) p.343-350. Tubingen Unversity Press.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekito, T., Okamoto, K., Suzuki, K.and Yoshida, K.: "Nucleotide sequence and secondary structure of four tRNA genes in mitochondrial DNA of Hansenula wiugei yest." Nucleic Acids Res.21. 3589-35890 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K., Yamada, K., Yamagiwa, Y., Kondo, T., Naganuma, K and Ozaki, K.: "Construction of a new pulse-controller for multi-electric field PFGE and separation of yeast chromosomal DNA." Seikagaku. 65 (in Japanese). 570-575 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K., Nishikawa, M., Inomata, K., Sekito, T.and Matsumoto, Y.: "Trans-kingdom conjugation as environmental response-Molecular mechanism of conjugation between prokaryotic bacteria and eukaryotic yeasts." in Gene Expression and Environmental Responses in Eukaryotic Microbes (ed by Yoshida, K.and Ootaki, T.), IGE16, p. 21-37, Tohoku University.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K.and Nishikawa, M.: "Molecular mechanism of trans-kingdom conjugation between Escherichia coli and yeasts. Protein" Nucleic Acid and Enzyme. 38 : (Review in Japanese). 61-68 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kohzuwa, T., Shidara, S., Yamaguchi, K.and Nakamura, N., Deligeer and Suzuki, S.: "Direct electrochemistry of copper-containing nitrite reductase from Achromobacter xylosoxidans NClB11015." Chemistry Letters. 1993. 2029-2032 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, K.and Yoshida, K.: "Organization of the fungal mitochondrial genomes. Protein, Nucleic Acid and Enzyme" 37, (Review in Japanese). 1110-1117 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekito, T., Okamoto, Kitano, H.and Yoshida, K.: "The structure and organization of mitochondeial genome in Hansenula wingei.l" Complete DNA sequence and gene organization. XV International Botanical Congress. ABSTRACTS. 503 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., Sekito, T., Kitano, H.and Yoshida, K.: "The structure and organization of mitochondeial genome in Hansenula wingei.l1. Gene structure and expression." XV International Botanical Congress.ABSTRACTS. 504 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, T., Nishino, N., Kai, Y., Harada, S., Ohshiro, Y., Suzuki, S., Kohzuma, T., Shidara, S.and Iwasaki, H.: "Crystallization and preliminary X-ray studies on Pseudoazurin from Achromobacter cycloclasters IAM1013." J.Biochem.114. 761-762 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshimura, T., Shidara, S., Ozaki, T.and Kamada, H.: "Five-coordinated nitrosylhemoprotein in the whole cells of denitrifying bacterium Achromobacter xylosoxidans NCIB11015" Arch. Mirobiol.160. 498-500 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohzuma, T., Takase, S., Shidara, S.and Suzuki, S.: "Electrochemical properties of copper proteins, pseudo-azurin and nitrite reductase from Achromobacter xylosoxidans NCIB11015" Chemistry Letters. 1993. 149-152 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, S., Kohzuma, T., Shidara, Ohki, K, and Aida, T.: "Novel spectroscopic aspects of type 1 copper in Hyphomicrobium nitrite reductase." Inorganica Chimica Acta.208. 107-109 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi, M., Yoshida, K.and Yanagishima, N.: "Sexual agglutination substances require a carrier glycoprotein for integration into the cell wall of Saccharomyces cerevisiae." Microbiology.140. 2217-2223 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inomata, K., Nishikawa, M.and Yoshida, K.: "The yeast Saccharomyces kluyveri as a recipient eukaryote : Bahavior of transmitted plasmids in transconjugants." j. Bact.176. 4770-4773

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., Sekito, H., Kitano, H.and Yoshida, K.: "Structure and diversityof yeast mitochondrial genomes. Protein." Nucleic Acid and Enzyme. 39 (Review in Japanese). 1638-1650 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, K., Yamagiwa, Y., Matsui, T.and Yoshida, K.: "Restriction enzyme-resistant high molecular weight telomeric DNA fragments in tabacco." DNA Res.1. 129-138 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okamoto, K., Sekito, T.and Yoshida, K.: "The mitochondrial genome of yeast Hansenula wingei encodes NADH dehyrogenase subunit genes ND4L and ND5." Mol. Gen. Genet.243. 473-476 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K.: "Trans-kingdom conjugation-A new gene introducing method for eukaryotic cells." Biosciense & Industry. 52. 812-817 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohzuma, T., Shidara, S.and Suzuki, S.: "Direct electrochemistry of nitrite reducatase from Achromobacter cycloclasters IAM1013." Bull. Chem. Soc. Jpn.67. 138-143 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekito, T., Okamoto, K., Kitano, H.and Yoshida, K.: "Yeast Hansenula wingei mitochondrial genome's complete DNA sequence demonstrated unique characteristics." Nucleic Acids Res. Symposium Series. 31. 233-234 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekito, T., Okamoto, K., Kitano, H.and Yoshida, K.: "The complete mitochondrial DNA sequence of Hansenula wingei reveals new characteristics of yeast mitochondria." Curr. Genet.27 (in press.). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M.and Yoshida, K.: "The trans-kindom conjugation more frequently induce the gene replacement than the transformation does in Saccharomyces cerevisiae yeast." (Submitted).(1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K.: "A new hypothesis of gene transfer in mitochondrion origin." Viba Origino.21. 74-76 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawasaki, Y., Inomata, K.and Yoshida, K.: "Trans-kingdom conjugation between Agrobacerium tumefaciens and Saccharomyces cerevisiae yeast." Plant & Cell Physiol.(Submitted). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekito, T., Okamoto, K., Kitano, H.and Yoshida, K.: "Clone bank of the mitochondrial genome of yeast Hansenula wingei." (Submitted). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, K., Nishikawa, M.and Katoh, A.: "Endosymbiotic conjugation : A new hypothetical mechanism for gene transfer to the host nucleus during the organelles origin." (Submitted). (1985)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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