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1994 Fiscal Year Final Research Report Summary

Fundamental study of development of autologous tissue fragmented vascular prosthesis for A-C bypass use

Research Project

Project/Area Number 04557060
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionYokohama city University School of Medicine

Principal Investigator

NOISHIKI Yasuharu  Yokohama City University School of Medicine, Assistant Professor, 医学部, 講師 (60033263)

Co-Investigator(Kenkyū-buntansha) YAMANE Yoshihisa  Tokyo University of Agriculture and Technology, Professor, 農学部, 教授 (50262225)
MIYATA Teruo  Koken Bioscience Institute, Director, 研究所, 所長
KONDO Jiro  Yokohama City University School of Medicine, Associate Professor, 医学部, 助教授 (00046038)
Project Period (FY) 1992 – 1994
KeywordsVascular prosthesis / Endothelial cell / Autologous tissue transplantation / Neointima / Bone marrow / Capillary formation / bFGF / Autocrine artificial Organ
Research Abstract

We developed a new method to accelerate the healing process of neointima formation of fabric vascular prostheses using autologous tissue fragment transplantation technology. This technology was applied to develop a small diameter vascular prostheses for aorta-coronary bypass surgery. As the autologous tissue, we used peripheral venous tissue, omentum tissue, subcutaneous adipose tissue and bone marrow tissue were adopted in this study. These tissues were chopped up and suspended. The suspension was enmeshed into the graft wall to entrap the tissue fragments in the interstices of the graft wall. All the grafts showed excellent healing of neointima with rapid endothelialization throughout the graft surface. Adipose tissue was useful in clinic since it was available in any surgical fields. Peripheral venous tissue contained major cell component of arterial wall. Therefore, the graft seeded with venous tissue fragments showed good healing. Omentum tissue revealed very active cell migration and proliferation. Bone marrow tissue showed different and special healing process as follows.
A bone marrow transplanted vascular graft functioned as an autocrine artificial organ which produced growth factors and was self-regulated. In a canine study, autologous bone marrow was harvested, enmeshed into a long-fibril-lenght ePTFE graft wall and implanted in the abdominal aorta. Active endothelialization started simultaneously throughout the graft, with numerous capillary ingrowth and was completed throughout the length within 3 weeks. Transplanted marrow cells survived and continued exogenous hemopoiesis with synthesis of bFGF which activated angiogenesis in the graft wall. A complete endothelial cell lining and hemopoiesis were maintained without adverse effects for up to 6 months.
From these results, we concluded that the grafts with autologous tissue fragmentation were promising candidates for aorta-coronary bypass surgery.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Y.Noishiki,Y.Tomizawa,Y.Yamane,T.Okoshi,A.Matsumoto: "Acceleration of neointima formation in vascular prostheses transplantation of autologous venous tissue fragments.Application to small-diameter grafts" J.Thoracic and Cardiovascular Surgery. 105. 796-804 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Noishiki,Y.Yamane,Y.Tomizawa,T.Okoshi,K.Imoto,A.Matsumoto: "Rapid neointima formation with elastic laminae similar to the natural arterial wall on an adipose tissue fragmented vascular prosthesis" American Society for Artificial Internal Organs. 40. 267-272 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Noishiki,Y.Tomizawa,Y.Yamane,A.Matsumoto: "Effectiveness of fragmented autologous adipose tissue as a sealer of porous textile grafts:Effects on endothelial development" J.Vascular Surgery. 20. 279-287 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Noishiki,Y.Yamane,Y.Tomizawa,A.Matsumoto: "Transplantation of autologous tissue fragments into an e-PTFE graft with long fibrils" Artificial Organs. 19. 17-26 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Matsumoto,Y.Noishiki,Y.Ichikawa,T.Soma,J.Kondo,T.Kosuge: "Sealing of fabric vascular prosthesis with autologous adpiose tissue:A preliminary report of its clinical application" Artificial Organs. 19. 51-56 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Satoh,S.Niu,Kanda,Y.Wada,T.Oka,Y.Noishiki: "Clinical use of low porosity woven ultrafine polyester fiber grafts" Artificial Organs. 19. 57-63 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Noishiki,H.Sung,C.Hata,S.Shen JMCintrye,P.Randall,D.Lin,M.Rawland,R.Quijano: "New horizone and the future of heart valve bioprostheses" International Association for cardiac Biological Implants, 290 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Noishiki,Y.Yamane,Y.Tomizawa,T.Okoshi,S.Satoh,M.Mo,J.Kondo,A.Matsumoto: "Heart Replacement Artificial Heart 4" International Symposyum on Artificial heart and Assist Devices, 424 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomizawa Y,Moon MR,Noishiki Y: "Antiadhesive membranes for cardiac reoperations [letter]" J Thorac Cardiovasc Surg. 107(2). 627-9 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sung HW,Tu R,Shen SH,Witzel TH,et al: "A newly developed porcine heart valve bioprosthesis fixed with an epoxy compound. An experimental evaluation" Asaio J. 40(2). 192-8 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Tomizawa Y,Yamane Y,Matsumoto A: "Effectiveness of fragmented autologous adipose tissue as a sealer of porous textile grafts : Effect on endothelial development" J Vasc Surg. 20. 279-87 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomizawa Y,Moon MR,Noishiki Y: "Dialdehyde starch-tanned bovine grafts in the coronary position [letter]" Ann Thorac Surg. 56(3). 593-5 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomizawa Y,Moon MR,Noishiki Y: "Biologic vascular grafts in the venous position [letter]" J Thorac Cardiovasc Surg. 107(3). 948-9 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sung HW,Witzel TH,Hata C,Tu R,Shen SH,Lin D,Noishiki Y,Tomizawa Y: "Quijano RC,Development and evaluation of a pliable biological valved conduit. Part II : Functional and hemodynamic evaluation" Int J Artif Organs. 16(4). 199-204 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Tomizawa Y,Yamane Y,Okoshi T,Satoh S,Matsumoto A: "Acceleration of neointima formation in vascular prostheses by transplantation of autologous venous tissue fragments. Application to small-diameter grafts" J Thorac Cardiovasc Surg. 105(5). 796-804 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Yamane Y,Tomizawa Y,Okoshi T,et al: "A functional neointima with regularly arranged smooth muscle cells in a fabric vascular prosthesis transplanted with autologous venous tissue fragments." Asaio J. 39(3). M746-M749 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Hata C,Tu R,Shen SH,et al: "Development and evaluation of a pliable biological valved conduit. Part I : Preparation, biochemical properties, and histological findings" Int J Artif Organs. 16(4). 192-8 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Yamane Y,Tomizawa Y: "Sealing of highly porous fabric vascular prostheses by adipose connective tissue fragments instead of preclotting with fresh blood" J Invest Surg. 6(3). 231-40 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki S,Kondo J,Imoto K,Kajiwara H,et al: "[Long-term results of closed mitral commissurotomy--comparative study of closed mitral commissurotomy (CMC), open mitral commissurotomy (CMC) and mitral valve replacement (MVR)]" Nippon Kyobu Geka Gakkai Zasshi. 41(9). 1460-6 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto K,Noishiki Y,Mo M,Kosuge T,Ishii M,Ichikawa Y,Imoto K,Kondo J,Matsumoto A: "[Disadvantages of "Gelseal graft" as a vascular prosthesis]" Kyobu Geka. 46(9). 762-6 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto K,Noishiki Y,Mo M,Tobe M,Imoto K.Kondo J,Matsumoto A: "[Unusual non-infective inflammatory responses around a collagen impregnated vascular prosthesis]" Kyobu Geka. 46(13). 1094-7 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Karube N,Noishiki Y,Yamamoto K,Kondo J,Matsumoto A: "Percutaneous insertion of a jacketed stent to close the entry of dissecting aortic aneurysms of DeBakey types I and II" Artif Organs. 17(12). 1004-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sung HW,Shen SH,Tu R,Lin D,Hata C,Noishiki Y,Tomizawa Y,Quijano RC: "Comparison of the cross-linking characteristics of porcine heart valves fixed with glutaraldehyde or cpoxy compounds" Asaio J. 39(3). M532-M536 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noishiki Y,Yamane Y,Tomizawa Y,Okoshi T,Satoh S,Wildevuur CR,Suzuki K: "Rapid endothelialization of vascular prostheses by seeding autologous venous tissue fragments" J Thorac Cardiovasc Surg. 104(3). 770-8 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto K,Noishiki Y,Mo M,Kondo J,Matsumoto A: "Unusual inflammatory responses around a collagen-impregnated vascular prosthesis" Artif Organs. 17(12). 1010-6 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomizawa Y,Noishiki Y,Okoshi T,Koyanagi H: "[Development of a small-caliber heparinized bovine graft]" Jpn J Artif Organs. 21. 100-103 (1992)

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      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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