1993 Fiscal Year Final Research Report Summary
A New Pulsed FAB Control System Coupled with an Array Detector on a Four-Sector Tandem Mass Spectrometer
| Project/Area Number |
04558022
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Osaka University |
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Principal Investigator |
SHIMONISHI Yasutsugu Inst.for Protein Res., Osaka Univ., Professor, 蛋白質研究所, 教授 (00029951)
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| Co-Investigator(Kenkyū-buntansha) |
KAMMEI Yoshihiro MS group, JEOL Ltd., Group leader, 質量分析開発グループ, グループ長
TAKAO Toshifumi Inst.for Protein Res., Osaka Univ., Instructor, 蛋白質研究所, 助手 (10197048)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Pulsed FAB / Array detector / Four-sector tandem mass spectrometer / Function generator / Initial ionization event / Sample lifetime / MS / MS sequencing / Femtomole |
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| Research Abstract |
A pulsed FAB has been proposed to be a powerful technique for dramatic enhancement in sensitivity and increase in sample lifetimes. Here we developed a new pulsed FAB control system coupled with a photodiode-based array detector on a four-sector tenden mass spectrometer.
The trigger pulse initially generated from the array detector control system is transmitted to the data acquisition processor via an optical fiber line, and them, to a function generator. The function generator is designed to provide a set of pulses of various repetitive frequencies and duty cycles. A high voltage power supply combined with the function generator allows a primary ion beam to be accelerated at up to a 6-kV potential with a quick rising and falling time less than 1 millisecond. The pulsing of primary ion beam is synchronized with start-up of the data acquisition on the array detector.
When the system was tested on the second MS using a pulsed Xe atom beam, the protonated molecular ion peaks of peptides were obtained with 10 times higher intensity than those by a continuous FAB in the initial ionization event. The detection limits for angiotensin I (m/z 1296.7) and substance P (1347.7) were about 10 femtomoles. This new pulsed FAB control system was further demonstrated to be effective for reducing sample amounts required for MS/MS sequencing of peptides to the subfemtomole order.
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[Publications] Agarwala, K., Kawabata, S., Takao, T., Murata, H., Shimonishi, Y., Nishimura, H., and Iwanaga, S.: "Activation Peptide of Human Factor IX Has Oligosaccharides O-Glycosidically Linked to Threonine residues at 159 and 169." Biochemistry. (in press). (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Fukada, Y., Matsuda, T., Kokame, K., Takao, T., Shimonishi, Y., Akino T., and Yoshizawa, T.: "Effects of Carboxyl Methylation of Photoreceptor G Protein gamma-Subunit in Visual Transduction." J.Biol.Chem.269. 5163-5170 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Fujisawa, Y., Muneoka, Y., Takahashi, T., Takao, T., Shimonishi, Y., Kubota, I., Ikeda, T., Minakata, H., Nomoto, K., Kiss, T., and Hiripi, L.: "An Invertebrate-Type Tachykinin Isolated from the Freshwater Bivalve Mollusc, Anodonta Cygnea." Peptide Chemistry. 1993. 161-164 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Kumazawa, S., Kanda, M., Aoyama, H., Utagawa, M., Kondo, J., Yoshikawa, N., Mikawa, T., Chiga, I., Hayase, T., Hino, T., Takao, T., and Shimonishi, Y.: "Structure of Aibellin, A New Peptide Antibiotic That Modifies Rumen Fermentation." Peptide Chemistry. 1993. 137-140 (1994)
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「研究成果報告書概要(欧文)」より
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[Publications] Morikawa, M., Daido, H., Takao, T., Murata, S., Shimonishi, Y., and Imanaka, T.: "A New Lipopeptide Biosurfactant Produced by Arthrobacter sp. Strain MIS38." J.Bacteriol.175. 6459-6466 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshino, K., Miyachi, M., Takao, T., Bag, P.K., Huang, X., Nair, G.B., Takeda, T., and Shimonishi, Y.: "Purification and Sequence Determination of Heat-Stable Enterotoxin elabolated by a Cholera Toxin-Producing Strain of Vibrio cholerae O1." FEBS Lett.326. 83-86 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Takao, T., Gonzalez, J., Yoshidome, K., Sato, K., Asada, T., Kammei, Y., and Shimonishi, Y.: "Automatic Precursor-Ion Switching in a Four-Sector Tandem Mass Spectrometer and Its Application to Acquisition of the MS/MS Product Ions Derived from a Partially ^<18>O-Labeled Peptide for Their Facile Assignments." Anal.Chem.65. 2394-2399 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Kanaji, T., Ozaki, H., Takao, T., Kawajiri, H., Ide, H., Motoki, M., and Shimonishi, Y.: "Primary Structure of Microbial Transglutaminase from Streptoverticillium sp. Strain S-8112." J.Biol.Chem.268. 11565-11572 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Takao, T., Kokame, K., Fukada, Y., Yoshizawa, T., Masatsugu, Y., Tanida, K., and Shimonishi, Y.: "Chemical and Mass Spectrometric Characterization of the Heterogeneous N-terminal Lipid Modification of Photoreceptor G-Protein alpha-Subunit." Proceedings of the 41th ASMS Conference on Mass Spectrometry and Allied Topics, San Francisco. 375 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Ikeda, T., Kubota, I., Miki, W., Nose, T., Takao, T., Shimonishi, Y., and Muneoka, Y.: "Structures and Actions of 20 Novel Neuropeptides Isolated from the Ventral Nerve Cords of an Echiuroid Warm, Urechis unicinctus." Peptide Chemistry. 1992. 583-585 (1993)
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