APROARCH TO RICE BACTERIAL BLIGHT RESISTANCE THROUGH MOLECULAR GENETICS

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04660008
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Breeding science
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: YOSHIMURA Atsushi KYUSHU UNIVERSITY, FUCULTY OF AGRICULTURE, ASOCIATE PROFESSOR, 農学部, 助教授 (00182816)
• Co-Investigator(Kenkyū-buntansha): IWATA Nobuo KYUSHU UNIVERSITY, FACULTY OF AGRICULTURE, PROFESSOR, 農学部, 教授 (50038204)
• Project Period (FY): 1992 – 1993
• Keywords: Rice bacterial blight resistance / RFLP marker / RAPD marker / Near-isogenec ines / Chromosome walk / Combining resisntance genes / Single seed descent method / Quantitative resistnace

Research Abstract

Genes for bacterial resistance of rice, Xa-1, Xa-3, Xa-4, xa-5, Xa-10 and Xa-11, were tagged with several RFLP and RAPD markers using near-isogenic lines and F2 populations. Xa-1 linked to several RFLP and RAPD markers with map distances of less than 5cM.Xa-3 and Xa-4 that are closely linked or allelic, linked to the same RFLP markers. Xa-10 showed the linkage with some RFLP and RAPD markers. The RAPD markeres linked to xa-5 and Xa-11 were also found. These RFLP and RAPD markers were useful for developing the combined lines of the bacterial blight resisitance genes.
We developed the recombinant inbred (RI) lines as a permanent mappinng population for RFLP/RAPD markers and used them to analyze the quantitative resistance to bacterial blight. The RFLP/RAPD land mark map constructed using the RI population covered approximately 1090 cM containg 114 RFLP loci and 26 RAPD loci. Three putative loci affecting the quantitative resistance were identified in the preliminary study.

Research Products

• [Publications] Yoshimura.S,A.Yoshimura,A.Saito,N.Kishimoto,M.Kawase,M.Yano,M.Nakagahra,T.Ogawa and N.Iwata: "RFLP analysis of introgressed chromosomal segments in three near-isogenic lines of rice for bacterial blight resistance genes,Xa-1,Xa-3 and Xa-4." Jpn.J.Genet.67. 29-37 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshimura.S,R.Nelson,A.Yoshimura,T.W.Mew and N.Iwata: "RFLP mapping of the bacterial blight resistance genes Xa-3 and Xa-4." Rice Genetics Newsletter. 9. 136-138 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshimura.S,A.Yoshimura,R.Nelson,T.W.Mew and N.Iwata: "Tagging genes for bacterial blight resistance in rice by RFLP and RAPD markers." Agricultural Biotechnology,Proceeding of Asia-Pacific Conference on Agricultural Biotechnology,China Sci.Tech.Press.202-205 (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tsunematsu.H,H.Hasegawa,S.Yoshimura,A.Yoshimura and N.Iwata: "Construction of an RFLP/RAPD linkage map by using recombinant inbred lines." Rice Genetics Newsletter. 10. 89-90 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshimura, S., A.Yoshimura, A.Saito, N.Kishimoto, M.Kawase, M.Yano, M.Nakagahra, T.Ogawa and N.Iwata: "RFLP anasysis of introgressed chromosomal sgments in three near isogenic lines of rice for bacterial blight resistance genes, Xa-1, Xa-3 and Xa-4." Jpn.J.Genet. 67. 29-37 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshimura, S., A.Yoshimura, R.Nelson, T.W.Mew and N.Iwata: "Tagging genes for bacterial blight resisntance in rice by RFLP and RAPD markers." Agricultural Biotechnology, Proceeding of Asia-Pacific on Agricultural Biotechnology, China Sci.Tech.Press. 202-205
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshimura, S., R.Nelson, A.Yoshimura, T.W.Mew and N.Iwata: "RFLP mapping of the bacterial blight resistance genes Xa-3 and XA-4." Rice Genetics Newsletter. 9. 136-138 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tsunematsu, H., H.Hasegawa, S.Yoshimura, A.Yoshimura and N.Iwata: "Construction of an RFLP/RAPD linkage map by using recombinant inbred lines." Rice Genetics Newsletter. 10. 89-90 (1993)
  • Description: 「研究成果報告書概要(欧文)」より