1993 Fiscal Year Final Research Report Summary
CELL BIOLOGICAL AND BIOCHEMICAL ANALYSIS OF THE STRUCTURE AND MECHANISM OF INSECT PHOTOPERIODISM
| Project/Area Number |
04660052
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
植物保護
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
TAKEDA Makio FACULTY OF AGRICULTURE, KOBE UNIVERSITY, ASSOCIATE PROFESSOR, 農学部, 助教授 (20171647)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ENDO Yasuhisa DEPARTMENT OF APPLIED BIOLOGY, KYOTO INSTITUTE OF TECHNOLOGY, 農学部, 教授 (50176806)
AIZONO Yasuo FACULTY OF AGRICULTURE, KOBE UNIVERSITY, PROFESSOR, 農学部, 教授 (20089931)
|
|---|
| Project Period (FY) |
1992 – 1993
|
| Keywords | catecolamines / photoperiodism / diapause / wild silkmoths / valine / ecdysteroids / adrenalin / dopamine |
|---|
| Research Abstract |
(1)Neurosecretory cells in the brain of Antheraea silkmoth showed immunohistochemical reactivities against antiopsin and anti-retinol binding protein, which shows the possibility that these cells are the photoperiodic receptors.
(2)HPLC-ECD analysis revealed that both adrenalin and dopamine contents peaked in the brain of Antheraea pernyi before ecdisteroids started being released into hemolymph. The result suggests that theses catecolamines are causally involved in the prothoracicotropic hormone(PTTH) release from the brain. These monoamine like immunohistochemical reactivities were located in the sites of both PTTH containing cells and bombyxin containing cells in the brain.
Diapause of A.pernyi is terminated by chilling. HPLC-ECD analysis revealed that low temperature affects monoamine metabolism. At intermediate temperatures MAO(monoamine oxidase) activity is high but at low temperatures NAT(N-acetyltransferase) is activated.
(3)We compared ecdysteroid titers both between under diapause maintaining photoperiod and under diapause terminating photoperiod and between the two Anteraea species. Diapause of both species was terminated by ten cycles of activating photoperiod but constitutively relatively high titer persisted in A.yamamai but the titer was at the base line level in A.pernyi when ecdysteroid release stopped. Hemolymph amino acid compositions of the two species were drastically different ; valine was the highest in A.yamamai but low in A.pernyi.
|
