RAPID DETECTION OF MYCOPLASMAS BY A FILTER-IMMUNOBINDING TECHNIQUE

1993 Fiscal Year Final Research Report Summary

• Project/Area Number: 04660333
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: Applied veterinary science
• Research Institution: MIYAZAKI UNIVERSITY
• Principal Investigator: SHIMIZU Takamasa MIYAZAKI UNIV., FAC.OF AGRICULTURE,PROFESSOR, 農学部, 教授 (10040825)
• Co-Investigator(Kenkyū-buntansha): NAGATOMO Hiroshi MIYAZAKI UNIV., FAC.OF AGRICULTURE,ASSIST.PROF., 農学部, 助教授 (10041063)
• Project Period (FY): 1992 – 1993
• Keywords: Mycoplasmas / Filter-immunobinding / Non-culture detection / イムノバインド反応

Research Abstract

A filter-immunobinding (FIB) technique in which commercial dot-plates and enforced nitrocellulose membrane, Nitro Plus 2000, were utilized, was developed. When diluted mycoplasma liquid cultures were used as antigens, it was critical to adsorb antisera with culture medium in order to avoid significant unspecific FIB reactions. Adsorbed antisera gave rise to specific FIB reactions with bovine and avian mycoplasma Type strains as well as field isolates identified by growth inhibition test. Rate of detection of M.bovirhinis and M.bovis from nasal discharge of calves by FIB was siightly less than by the routine culture method. Detection rate of M.gallisepticum and M.synoviae by FIB much less than that obtained by culture method, probablly due to small CFU of mycoplasmas existed in the oropharynxes of normal chickens examined. It was concluded that FIB technique was useful for the rapid diagnosis of mycoplasmoses in which great number of organisms might exist.