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1993 Fiscal Year Final Research Report Summary

Characterization of the HTLV-I receptor

Research Project

Project/Area Number 04670276
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 蚕糸学
Research InstitutionKumamoto University School of Medicine

Principal Investigator

KANNAGI Mari  M.D.Kumamoto University School of Medicine, Department of Biodefense and Medical Virology, Associate Professor, 医学部, 助教授 (80202034)

Project Period (FY) 1992 – 1993
KeywordsHTLV-I / Virus receptor / Syncytium / Viral adsorption
Research Abstract

Human T-cell leukemia virus type-I (HTLV-I) and human immunodeficiency virus type-1 (HIV-1) have a similar tropism for target cell types, especially for CD4+ T cells. Although CD4 is an HIV-1 receptor, the cellular receptor for HTLV-I is not yet known. HTLV-I does not effectively infect cells as a cell free virus, but require a cell-to-cell contact between HTLV-I infected cells and target cells. Initially, we used a syncytium formation system to detect HTLV-I infection. This system provided us an evidence that the receptors for HTLV-I and HIV-1 are totally independently present on the cell surface of human T cells. Furthermore, HTLV-I receptor seemed not to be involved in the CD4 molecule. We tried to make monoclonal antibodies for the HTLV-I receptor by using this system, but an antibody blocking HTLV-I-induced syncytium formation was not obtained yet. Besides such cell-to-cell system, we established an experimental system to detect HTLV-I adsorption, since the cell-to-cell would be more affected by adhesion molecules on the cell surface unrelated to virus receptors. HTLV-I particles were concentrated and incubated with the target cells' and viral adsorption to the cell surface was quatnitated by FACS analysis. HTLV-I could bind a wide range of human cell lines with a few exceptions. Human anti-HTLV-I sera inhibited the HTLV-I adsorption, suggesting that these sera contain antibodies neutralizing HTLV-I binding. Efforts to obtain monoclonal antibodies to the HTLV-I receptor are currently being made using these systems.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] M.Kannagi,et al.: "Cytotoxic T cell response and expression of the target antigen in HTLV-I infection." Leukemia. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.A.El-Farrash,et al.: "The mycoplasma-related inhibitor of HIV-1 reverse transcriptase is a DNase present in the particle free supernatants." J.Virol.Methods. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kannagi,et al.: "Expression of the target antigen for cytotoxic T lymphocytes on adult T cell leukemia cells." Int J.Cancer. 54. 582-588 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Arari,et al.: "Simple assay system for detecting human T cell leukemia virus type-I-binding cells and its application in titrating binding inhibitory antibodies." Laboratory Investigation. 69. 629-634 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.J.Kuroda,et al.: "Inefficient transmission of HTLV-I to MOLT-4 cells by cell-free virus and co-cultivation." Intervirology. 34. 202-212 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kannagi,et al.: "Target epitope in the tax protein of human T cell leukemia virus type I recognized by class I major histo-compatibility complex-restricted cytotoxic T cells." J.Virol.66. 2928-2933 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Kannagi: "Target epitope in the taxt protein of human T cell leukemia virus type I recognized by class I major histocompatibility complex-restricted cytotoxic T cells." J.Virol.66. 2928-2933 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.J.Kuroda: "Inefficient transmission of HTLV-I to Molt-4 cells by cell-free virus and cocultivation." Intervirology. 34. 202-212 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Akari: "Sinple assay system fordetecting human T cell leukemia virus type I-bindin cells and its application in titrating binding inhibitory antibodies." Laboratory Investigation. 69. 629-634 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kannagi: "Expression of the target antigen for cytotoxic T lymphocytes on adult T lymphocytes on adult T cell leukemia cells." Int.J.Cancer. 54. 582-588 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.A.El-Farrash: "The mycoplasma-related inhibitor of HIV-1 reverse transcriptase is a DNase present in the particle free supernatant." J.Virol.Methods. in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Kannagi: "Cytotoxic T cell response and expression of the target antigen in HTLV-I infection." Leukemia. in press. (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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