1994 Fiscal Year Final Research Report Summary
Detection of p53 gene mutations in fine-needle aspiration biopsy specimens from suspected breast cancers by polymerase chain reaction-single strand conformation polymorphism analysis.
| Project/Area Number |
04670743
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Agro-economics
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| Research Institution | Sapporo Medical University School of Medicine |
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Principal Investigator |
SATO Takashi Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 講師 (50205928)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Shuji Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 助手 (40231394)
OKAZAKI Akira Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 助手 (50213921)
OKAZAKI Minoru Sapporo Med.Univ.School of Medicine, Assistant prof., 医学部, 講師 (30160667)
HIRATA Koichi Sapporo Med.Univ.School of Medicine, Professor., 医学部, 教授 (50136959)
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| Project Period (FY) |
1992 – 1994
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| Keywords | Breast cancer / Aspiration biopsy / PCR-SSCP / p53 / Point mutation / PCR / SSCP |
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| Research Abstract |
The best means of reducing the mortality rate in the breast cancer patients is the early detection of breast cancers, and fine-needle aspirated breast tissue biopsy is one method to achieve this purpose. However, false-negative or false-positive diagnoses invariably occur and this can detract from the usefulness of the technique. Therefore, to obtain greater diagnostic accuracy with aspirated breast tissue specimens suspected of being cancers, we have evaluated the use of p53 gene mutation to identify cancer cells.
Genomic DNA was extracted from aspiration biopsy specimens taken from 15 suspected cases of breast cancer, including 7 known cases of breast cancer, and the p53 gene was studies for evidence of mutation by using a polymerase chain reaction-single strand conformation polymerphism (PCR-SSCP) analysis. In 5 of the 15 cases (33%), p53 gene mutation was identified and these tumors were subsequently histologically diagnosed as malignant. Further, DNA flow cytometry of the 15 tumors demonstrated that 6 (40%) were aneuploid and malignant, whereas 9 (60%) were diploid and benign. It was also found that the tumor cells in 5 aspirated cases that showed p53 gene mutations were all aneuploid, the p53 protein expression was positive, and the tumors were proved to be histologically malignant. It was thus concluded that the detection of p53 gene mutation by PCR-SSCP analysis of aspirated biopsy specimens from suspected breast cancers is a useful method for achieving a more accurate diagnosis.
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