1993 Fiscal Year Final Research Report Summary
Application of monoclonal antibody against solid tumor to boron neutron capture therapy model
| Project/Area Number |
04670765
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUJII Yuzo Institute of Medical Science, University of Tokyo, Assistant, 医科学研究所, 助手 (40143515)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Hisao Institute of Atomic Energy, Rikkyo University, Professor, 原子力研究所, 教授 (10062605)
NARIUCHI Hideo Institute of Medical Science, University of Tokyo, Professor, 医科学研究所, 教授 (10012741)
TOMITA Toshio Institute of Medical Science, University of Tokyo, Assistant, 医科学研究所, 講師 (00126129)
SAEGUSA Yoshiyuki Institute of Medical Science, University of Tokyo, Clinical Fellow, 医科学研究所, 医員
YANAGIE Hironobu Institute of Medical Science, University of Tokyo, Instructor, 医科学研究所, 教務職員 (30212278)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Boron neutron capture therapy (BNCT) / ^<10>B-compound / Thermal neutron / Anti-CEA monoclonal antibody / Immunoliposome |
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| Research Abstract |
An immunoliposome containing a ^<10>B compound has been examined as a selective drug delivery system in boron neutron capture therapy.
Immunoliposome was prepared by conjugating anti-CEA monoclonal antibody (MoAb) with liposomes containing ^<10>B compound. The immunoliposome were shown to bind selectively to human pancreatic carcinoma cells (AsPC-1) bearing CEA on their surface. Boronated anti-CEA immunoliposome exhibited the suppression of tumor cell growth in vitro selectively upon thermal neutron irradiation. The control boronated anti-DNP immunoliposome could not show the suppression.
The tumor growth of inplanted AsPC-1 cells after injection of ^<10>B-immunoliposome was suppressed upone thermal neutron irradiation in in vitro. Histopathologically, hyalinization and necrosis were found in the tumor tissues. The tumor tissue injected with saline-containing immunoliposome and irradiated showed neither destruction nor necrosis. The tumor growth suppression treated with ^<10>B-immunoliposome was stronger than the groups treated with ^<10>B-compound solutions or MoAb-free 10B containing liposome upon thermal neutron irradiation.
The results suggested that boronated immunoliposome can deliver a great amount of ^<10>B atoms to the tumor cells selectively and exert cytotoxicity and tumor growth suppression on them upon thermal neutron irradiation.
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[Publications] H.Yanagie, Y.Fujii, K.Kosakai, Y.Saegusa, M.Eriguchi, S.Mikamo, Y.Takeda, I.Yoshizaki, N.Akiyama, Y.Nonaka, H.Hasebe, T.Tomitaq and H.Kobayashi: "Effect of Intratumoral Injection of ^<10>B-immunoliposome on BNCT for Growth Inhicition of Human Pancreatic Cancer Grafts in Nude Mice." Biotherapy. (in press). (1994)
Description
「研究成果報告書概要(欧文)」より
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[Publications] H.Yanagie, Y.Fujii, Y.Saegusa, M.Eriguchi, S.Mikamo, Y.Takeda, I.Yoshizaki, N.Akiyama, Y.Nonaka, H.Hasebe, T.Tomita and H.Kobayashi: "In vitro and in vivo boron neutron capture therapy model using drug delivery system." Journal of Japanese Research Society for Gastroenterological Carcinogenesis. 5. 117-120 (1993)
Description
「研究成果報告書概要(欧文)」より
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