1993 Fiscal Year Final Research Report Summary
A cancer-reactive human monoclonal antibody derived from a colonic cancer patient treated with localimmunotherapy
| Project/Area Number |
04670780
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
MONDEN Takushi Osaka University Assistant Professor, 医学部, 助手 (20174477)
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| Co-Investigator(Kenkyū-buntansha) |
OUE Masakuki Osaka University M.D., 医学部・付属病院, 医員
TAKEDA Tsutomu Osaka University Assistant Professor, 医学部, 助手 (80236471)
KOBAYASHI Tetsuro Osaka University Assistant Professor, 医学部, 助手 (40162002)
SHIMANO Takashi Osaka University Assistant Professor, 医学部, 講師 (80144476)
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| Project Period (FY) |
1992 – 1993
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| Keywords | human monoclonal antibody / local immunotherapy / colon cancer / cell surface antigen / sialylated lacto-series |
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| Research Abstract |
A human monoclonal antibody, YJ-37 (IgM) was generated through thefusion of human B lymphoblastoid cell line HO-323 with the regional lymph node lymphocytes from a clonic cancer patient who was treated with a local immunotherapy. This antibody was purified and conjugated with biotin, after which direct immunohistochemical staining was performed. The results revealed that YJ-37 selectively reacted with colonic cancer (7/19), gastriccancer (7/13), but not with normal epithelia. Membrane immunofluorescence and FACS analysis also showed that YJ-37 bound to tumor cell surfaces. Furhermore, the chemical structure of the antigen defined by YJ-37 was analyzed by means of thin-layr chromatography immunostaining and ELSA.The results indicated that YJ-37 reacted with sialylated lacto-series carbohydrate chains, which have been reported to accumulate in cancer cells.
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