1993 Fiscal Year Final Research Report Summary
Single lung transplantation for canine pulmonary hypertension
| Project/Area Number |
04670828
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kobe University School of Medicine |
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Principal Investigator |
YAMASHITA Chojiro Kobe University Associate Professor, 医学部, 助手 (00144569)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Masayoshi Kobe University Professor, 医学部, 教授 (70030856)
ISHII Noboru Kobe University Professor, 医学部, 講師 (10168172)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Eisenmenser syndrome / Lung transplantation / dog / Pulmonary hypertension |
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| Research Abstract |
Background : Despite recent reports of the clinical application of single lung transplantation for pulmonary hypertension, there is little underlying experimental data because of the lack of a reliable animal pulmonary hypertensive transplant model. We have established a pulmonary hypertensive model in beagles using dehydromonocrotaline, and have been able to accurately measure cardiopulmonary hemodynamics and use circulatory assists during procedures. The purpose of this study was to determine whether single lung transplantation could be performed following the protocol of clinical procedure.
Methods and Results : In six control dogs, allograftings were successfully completed without cardiopulmonary bypass. Since one pulmonary hypertensive recipient dogs died of right ventricular failure during the procedure without cardiopulmonary bypass, we used bypass for allograftings in five pulmonary hypertensive dogs. Cardiopulmonary bypass lowered pulmonary artery pressure allowing pulmonary arterial clamping and avoiding right ventricular overload. All pulmonary hypertensive dogs on bypass were successfully weaned from bypass, indicating a good hemodynamic response to transplantation. In pulmonary hypertensive dogs, transplantation resulted in significant decreases in systolic pulmonary artery pressure and pulmonary vascular resistance, and a significant increase in blood flow to the graft lung, whereas in controls the results were the reverse.
Conclusions : Thus, we were able to show that hemodynamics improved following single lung transplantation with cardiopulmonary bypass in a new pulmonary hypertensive animal model. These relatively larger animals are valuable for further studies of single, double, bilateral, and heart-lung transplantation for pulmonary hypertension.
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