1993 Fiscal Year Final Research Report Summary
Gene expression of inflammatory cytokines in periodontal tissues and network of thier cytokines
| Project/Area Number |
04671107
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University, School of Dentistry |
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Principal Investigator |
HANAWAWA Shigemasa Meikai University, School of Dentistry associate Professor, 歯学部, 助教授 (60060258)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Akira Meikai University, School of Dentistry associate Professor, 歯学部, 助手 (70236454)
AMANO Shigeru Meikai University, School of Dentistry associate Professor, 歯学部, 講師 (90167958)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Periodontal tissues / Cytokine / Gene expression / Signal transduction / Network mechanism |
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| Research Abstract |
We have previously demonstrated that lipopolysaccharide and finfriae derived from periodotal pathogens induces strongly gene expression of several inflammatory cytokines in cell components of periodontal tissues.
Recently, many studies have shown that IL-1, TNF-alpha, and IL-6 werepotent stimulater for bone resortptoon. Therefore, these cytokines may play an important role in initiation and development of adult periodontal disease which is characterized in inflammation of gingiva and alveolar bone resorption.
In the present study, we studiesd gene expression of inflammaytory cytokines of gingival fibroblasts and osteoblastsic cells treated by cytokine, and further signal transducing mechanism of their gene expressions. The following results was obtaind in this study. 1) Marked gene expression of monocyte chemoattractant MCP-1 was observed in gingival tissues of adult periodontal patients. 2) TNF-alpha induces expression of monocyte chemoattractant JE/MCP-1 via fos and jun genes in mouse osteoblastic cells MC3T3-E1 cells. 39) IL-1 induces expression of monocyte chemoattractant JE/MCP-1 in mouse osteoblastic cells and human gingival fibroblasts.
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[Publications] Takeshita, A., Niu, Z.G., Hanazawa, S., Takara, I., Higuchi, H., Katayama, I.and Kitano, S.: "Effect of interleukin-1 beta gene expression an function of fibroblastic cells derived from human periodontal ligament." J.Periont., Res.27. 250-255 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Hanazawa, S., Takeshita, A., Amano, S., Semba, T., Niratuka, T., Karo, H., and Kitano, S.: "TNF-alpha induces expression induced in momocyte chemoattractant JE via fos and jun genes in clonal osteoblastic MC3T3-E1 cells" J.Biol. Chem.265(13). 9526-9532 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Murakami, Y., Hanazawa, S., Nishida, K., Iwasaka, H., and Kitano, S.: "N-acethyl-D -Galactosamine inhibits TNF-alpha gene expression induced in mouse peritoneal macrophages by fimbriae of Porphyromonas gingivalis, an oral anaerobe." Biochem, Biophys. Res. Commun. 192(2). 826-832 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Hanazawa, S., Kawata, Y., Takeshita, A., Kumada, H., Okithu, M., Tanaka, S., Yamamoto, Y., Masada, T., Umenoto, T. and Kitano, S.: "Expression of monocyte chemoattractant MCP-1 IN adult periodontal disease : Increased monocyte chemotactic activity increbicular fluids an dinduction of MCP-1 expression in gingival tissues." Infect, Immun.61(12). 5419-5224 (1993)
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[Publications] A., Amano, Hanazawa, S., Kawata, Y., Miyata, Y., and Kitano, S.: "Phorbol Myristate acetate stimulates osteoclast fromation in 1a, 25(OH)2D3 prined mouse embronic calvarial cells by prostaglandin-dependent mechanism" J.Bone Mine. Res.(in press). (1994)
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「研究成果報告書概要(欧文)」より
