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1994 Fiscal Year Final Research Report Summary

Population biology and molecular systematics of northern temperate elements of Arcto-Tertiary origin in North America

Research Project

Project/Area Number 05041090
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionKyoto University

Principal Investigator

KAWANO Shoichi  Professor, Kyoto Faculty of Science, University, 理学部, 教授 (30019244)

Co-Investigator(Kenkyū-buntansha) SOLTIS P.s.  Professor, Dept.of Botany, Washington State University, プルマン市 ワシントン州立大学, 教授
SOLTIS D.e.  Professor, Dept.of Botany, Washington State University, プルマン市 ワシントン州立大学, 教授
WHIGHAM D.  Senior Ecologist, Smithsonian Environmental Research Center, スミソニアン環境研究センター, 主任研究員
UTECH F.h.  Head, Section of Botany, Carnegie Museum of Natural History, カーネギー自然史博物館・植物部門, 主任研究員
MORITA Tatsuyoshi  Professor, Faculty of Education, Niigata University, 教育学部, 教授 (30115084)
TAKASU Hideaki  Asso.professor, Faculty of Education, Wakayama University, 教育学部, 助教授 (90108001)
OHARA Masashi  Asst.professor, Faculty of Agriculture, Hokkaido University, 農学部, 助手 (90194274)
TAJIMA Fumio  Asst.professor, Department of Population Genetics, National Institute of Genetic, 集団遺伝研究系, 助手
TERACHI Toru  Lecturer, Faculty of Engineering, Kyoto Sangyo University,, 国土利用開発研究所, 講師 (90202192)
MIYASHITA Naohiko  Asst.professor, Faculty of Agriculture, Kyoto University, 農学部, 助手 (20212243)
TERAUCHI Ryohei  Asst.professor, Faculty of Science, Kyoto University, 理学部, 助手 (50236981)
Project Period (FY) 1993 – 1994
KeywordsLiliiflorae / Arcto-Tertiary element / chloroplast DNA / rbcL gene / Adh gene / mitochondria DNA / Saxfragaceae / evolutionary distance
Research Abstract

1. Sequences of the rbcL gene (cpDNA) (1380bp) were analyzed for 98 taxa of Liliiflorae, which show disjunct geographical distributions between Japan and western and/or eastern North America. These taxa studied are representatives of northern temperate elments of Arcto-Tertiary origin. Molecular phylogenetic status of these Liliiflorae taxa and their affinities were critically analyzed. Several noteworthy and significant findings were made as to the systematic status of North American as well as Asiatic taxa. Melanthiales-Heloniadeae proved to be very heterogeneous, Tofieldia and Japonolirion being at the root of the Monocots, while other genera are scattered over several different clades.
2. cDNA of phosphoglucoisomerase (Pgi) was determined for Dioscorea tokoro, a wild yam (Dioscoreaceae). Microsatellite polymorphism of D.tokoro was also analyzed.
3. In Brassicaceae, adh gene (2.3kb) of Arabis gemmifera was sequenced. Outstandingly high rates of nuceotide substitutions (61), insertion … More and deletion (25) were detected from populations of this species. In Raphanus and Helianthus, cloning and restriction mapping of mitochondrial DNA fragments containing atpA gene were conducted, and cytoplasmic male sterility was examined by using a new anlaytic method. This new approach will be useful for future molecular phylogenetic analysis as well molecular population biology. In Compositae, a unique gene duplication was newly detected in 6PGDH of Aster (Asterinae) from Japan and eastern North America. Several disjunct groups of Saxifragaceaeand Hydrangeaceae between Japan and North America were also analyzed based on the sequencing data of rbcL and matK genes.
4. New methods have been developed to estimate evolutionary distance for reconstructing molecular phylogenetic trees based upon nuceotide sequence data. Statistical methods were designed for estimating the effective number of alleles, expected heterozygosity and genetic distance in self-incompatibility locus. These new analytical methods are most useful for future molecular evolutionary analyzes. Less

  • Research Products

    (68 results)

All Other

All Publications (68 results)

  • [Publications] Kudoh,H.: "Phenotypic variabilities in life history traits and phenology of field populations of Cardamine flexuosa and C.fallax(Cruciferae)in Honshu,Japan" Plant Species Biol.8. 7-20 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshie,F.: "Seasonal changes in nitrogen fractions Copitis japonica,a temperate forest evergreen hemicryptophyte,and their implications" Ann.Bot.74. 217-226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinwari,Z。K.: "Recognition of the New World Disporum section Prosartes and Prosartes (Liliacea based on the sequence data of rbcL gene" Taxon. 43. 353-366 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinwari,Z.K.: "Phylogenetic relationships among genera in the Liliaceae-Aparagoideae-Polygonatae sensu lato inferred from rbcL gene sequence data." Pl.Syst.Evol.192. 263-277 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinwari,Z.K.: "Molecular systematics of the Liliace tribe Polygonateae.I.RFLP analysis of cpDNA in several Asiatic Disporum species" Plant Species Biol.9. 11-18 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishiguri,Y.: "Effects of different photoperiods and chilling treatments on growth and reproduction of Cardaine flexuosa With.and its ecological implicaltions" Plant Species Biol.9. 75-84 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Utech,F.H.: "Biosystematic studies in Disporum (Liliaceae-Asparagoideae-Polygonateae)VI.Recognition of the North American Section Prosartes and an autonomous genus" Mem.Fac.Sci.,Kyoto Univ.16. (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinwari,Z.K.: "Molecular systematics of the genus Uvularia and related taxa based upon rbcL gene sequence data" Plant Species Biol.10(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano,S.: "A study on the natural hybrid swarms of two Artemisia species (Compositae).A.capillaris and A.japonica in central Honshu,Japan" J.Phytogeogr.& Taxon. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,H.: "Molecular systematics of the Trilliaceae sensu lato as inferred from rbcL sequence data" Molecular Phylogenetics and Evolution. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,H.: "Evolutionary biology of Trillium and related genera (Trilliaceae) I.Restriction site mapping and variation of chloroplast DNA and its systematics implications" Plant Species Biol.10(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Thien,L.B.: "Fluorescent Magnolia flowers" Plant Species Biol.10(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano,S.: "Pollination biology of Oenothera (Onagraceae).The interplay between floral UV-absorbancy patterns and floral volatiles as signals to nocturnal insects" Plant Species Biol.10(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano,S.: "Optimal strategy between propagule output,propaguel size and cost of propagule production in plants with special reference to its evolutionary-ecological implications" Plant Species Biol.10(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawano,S.: "Evolutionary biology of Trillium and genera (Trilliaceae) II.Cladistic analyses on gross morphological characters and phylogeny of the genus Trillium" Plant Species Biol.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terauchi,R.: "Microsatellite polymorphism in Dioscorea tokoro,a wild yam species" Genome. 37. 794-801 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terauchi,R.: "A polymorphic microsatellite marker from the tropical tree Dryobalanops lanceolata (Diptercarpaceae)" Japanese Journal of Genetics. 69. 567-576 (1944)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyashita,N.: "Molecular population genetics of chloroplast genes in ancestral species of wheat" Proc.8th Int.Weat Symp.Beijing. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyashita,N.T.: "Molecular variation in chloroplast DNA regions in ancestral species of theat" Genetics. 137. 883-889 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Statistical method for estimating the effective number of alleles,expected heterozygosity and genetic distance in self-incompatibility locus" Jpn.J.Genet.69. 287-295 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Unbiased estimation of evolutionary distance between nucleotide sequences" Mol.Biol.Evol.10. 677-688 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Simple methods for testing the molecular evolutionary clock hypothesis" Genetics. 135. 599-607 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Statistical analysis of DNA polymorphism" Jpn.J.Genet.68. 567-595 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Estimation of evolutionary distance for reconstructing molecular phylogenetic trees" Mol.Biol.Evol.11. 278-286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akimoto,M.: "Genetic structure of natural populations of Oryza glumaepatula distributed in the Amazon basin." Rice Genetic News Letter. 94(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohara,M.: "Some ecological and demographic characteristics of two growth of wild soybean (Clycine soja Sieb.et Zuee.)" Can.J.Bot.72. 486-492 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohara,M.: "Effects of inflorescence size on visits from pollinators and seed set of Corydalis ambigua (Papaveraceae)" Oecologia(Berlin). 98. 25-30 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akhter,S.: "Clonal Diversity in the Agamospermous Polyploids of Taraxacum hondoense in Northern Honshu,Japan" J.Plant Res.106. 167-179 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishino,T.: "Biosystematic studies on the Kalimeris species (Compositae) in Niigata Prefecture.I.Winter leaves and branching pattern" J.Jpn.Bot.69. 226-234 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishino,T.: "6-Phosphogluconate Dchydrogenase (6PGD) Gene Duplication in Kalimeris (Asteraceae)" Plant Species Biol.9. 91-97 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita,T.: "Phytogeography and speciation of Taraxacum (Asteraceae) in East Asia" Korean Journal of Plant Taxonomy. 24. 145-155 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matthews,P.: "The genetics of agriculture: DNA variation in taro and yam.In: Hather,J.G.(ed.),Modern Techniques and Applicaions in the Archaeobotany of Tropical Agriculture" Unwin and Hyman,London, 251-262 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsunewaki,K.: "Use of RFLP analyses for wheat germplasm evaluation.In: Damania,A.B.(ed.),Biodiversity and Wheat Improvement" John Wiley and Sons,Inc.,Chichester, 17-31 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tajima,F.: "Measurement of DNA polymorphism,In: Takahata,N.and Clark,A.G.(eds.),Mechanisms of Molecular Evolution" Japan Sci.Soc.Press,Tokyo/Sinauer Associates,Sunderland,MA., 37-59 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kudoh, H., Ishiguri, Y.and Kawano, S.: "Phenotypic variabilities in life history traits and phenology of field populations of Cardamine flexuosa and C.fallax (Cruciferae) in Honshu, Japan." Plant Species Biol.8. 7-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshie, F., Nakashima, H., Arai, H.and Kawano, S.: "Seasonal changes in nitrogen fractions of Copitis japonica, a temperate forest evergreen hemicryptophyte, and their implications." Ann.Bot.74. 217-226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinwari, Z.K., Terauchi, R., Utech, F.H.and Kawano, S.: "Recognition of the New World Disporum section Prosartes and Prosartes (Liliaceae) based on the sequence data of rbcL gene." Taxon. 43. 353-366 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinwari, Z.K., Kato, H., Terauchi, R.and Kawano, S.: "Phylogenetic relationships among genera in the Liliaceae-Aparagoideae-Polygonatae sensu lato inferred from rbcL gene sequence data." Pl.Syst.Evol.192. 263-277 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinwari, Z.K., Kato, H., Terauchi, R.and Kawano, S.: "Molecular systematics of the Liliaceae tribe Polygonateae. I.RFLP analysis of cpDNA in several Asiatic Disporum species." Plant Species Biol.9. 11-18 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishiguri, Y., Kudoh, H.and Kawano, S.: "Effects of different photoperiods and chilling treatments on growth and reproduction of Cardamine flexuosa With. and its ecological implications." Plant Species Biol.9. 75-84 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Utech, F.H., Shinwari, Z.K.and Kawano, S.: "Biosystematic studies in Disporum (Liliaceae-Asparagoideae-Polygonateae) VI.Recognition of the North American Section Prosartes and an autonomous genus." Mem.Fac.Sci., Kyoto Univ.16. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinwari, Z.K., Utech, F.H., Whigham, D.H., Terauchi, R.and Kawano, S.: "Molecular systematics of the genus Uvularia and related taxa based upon rbcL gene sequence data." Plant Species Biol.10 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano, S., Hoshiya-Ushida, S.and Nagai, Y.: "A study on the natural hybrid swarms of two Artemisia species (Compositae). A.capillaris and A.japonica in central Honshu, Japan." J.Phytogeogr.& Taxon. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, H., Terauchi, R.Utech, F.H.and Kawano, S.: "Molecular systematics of the Trilliaceae sensu lato as inferred from ubcL sequence data." Molecular Phylogenetics and Evolution. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, H., Kawano, S., Terauchi, R., Ohara, M and Utech, F.H.: "Evolutionary biology of Trillium and related genera (Trilliaceae) I.Restriction site mapping and variation of chloroplast DNA and its systematics implications." Plant Species Biol.10 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Thien, L.B., Kawano, S., Latimer, S., Devall, M.S., Rosso, S., Azuma, H., Jobes, D.: "Fluorescent Magnolia flowers." Plant Speceis Bilogy. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyashita, N.T., Mori, N.and Tsunewaki, K.: "Molecular variation in chloroplast DNA regions in ancestral sepcies of wheat." Genetics. 137. 883-889 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F., Tokunaga, T.and Miyashita, N.T.: "Statistical method for estimating the effective number of alleles, expected heterozygosity and genetic distance in self-incompatibility locus." Jpn.J.Genet.69. 287-295 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F.: "Unbiased estimation of evolutionary distance between nucleotide sequences." Mol.Biol.Evol.10. 677-688 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F.: "Simple methods for testing the molecular evolutionary clock hypothesis." Genetics. 135. 599-607 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F.: "Statistical analysis of DNA polymorphism." Jpn.J.Genet.68. 567-595 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F.and Takezaki, N.: "Estimation of evolutionary distance for reconstructing molecular phylogenetic trees." Mol.Biol.Evol.11. 278-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akimoto, M., Ohara, M., Morishima, H.and Shimamoto, Y.: "Genetic structure of natural populations of Oryza glumaepatul distibuted in the Amazon basin." Rice Genetic News Letter. 94 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohara, M.and Shimamoto, Y.: "Some ecological and demographic characteristics of two growth of wild soybean (Glycine soja Sieb.et Zucc.)." Can.J.Bot.72. 486-492 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohara, M.and Higashi, S.: "Effects of inflorescence size on visits from pollinators and seed set of Corydalis ambigua (Papaveraceae)." Oecologia (Berlin). 98. 25-30 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akhter, S., Morita, T.and Yoshida, Y.: "Clonal Diversity in the Agamospermous Polyploids of Taraxacum hondoense in Nothern Honshu, Japan." J.Plant Res.106. 167-179 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishino, T.and Morita, T.: "Biosystematic studies on the Kalimeris species (Compositae) in Niigata Prefecture. I.Winter leaves and branching pattern." J.Jpn.Bot.69. 226-234 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishino, T.and Morita, T.: "6-Phosphogluconate Dchydrogenase (6PGD) Gene Duplication in Kalimeris (Asteraceae)." Plant Species Biol.9. 91-97 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano, S., Odaki, M., Yamaoka, R., Oda-Tanabe, M., Takeuchi, M.and Kawano, N.: "Pollination biology of Oenothera (Onagraceae). The interplay between floral UV-absorbancy patterns and floral volatiles as signals to nocturnal insects." Plant Species Biol.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano, S.and Hara, T.: "Optimal strategy between propagule output, propagule size, and cost of propagule production in plants with special reference to its evolutionary-ecological implications." Plant Species Biol.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawano, S.and Kato.H.: "Evolutionary biology of Trillium and related genera (Trilliaceae) II.Cladistic analyzes on gross morphological characters and phylogeny of the genus Trillium." Plant Species Biol.(submitted).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terauchi, R.and Konuma, A.: "Microsatellite polymorphism in Dioscorea tokoro, a wild yam species." Genome. 37. 794-801 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terauchi, R.: "A polymorphic microsatellite marker from the tropical tree Dryobalanops lanceolata (Diptercarpaceae)." Japanese Journal of Genetics. 69. 567-576 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyashita, N., Mori, N.and Tsunewaki, K.: "Molecular population genetics of chloroplast genes in ancestral species of wheat" Proc.8th Int.Wheat Symp.Beijing.(1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, T.: "Phytogeography and speciation of Taraxacum (Asteraceae) in East Asia." Korean Journal of Plant Taxonomy. 24. 145-155 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matthews, P.and Terauchi, R.: Unwin and Hyman, London. The genetics of agriculture : DNA variation in taro and yam In : Hather, J.G.(ed.), Modern Techniques and Applications in the Archaeobotany of Tropical Agriculture, 251-262 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunewaki, K., Liu, Y.-G., Takumi, S., Mori, N., Nakamura, H., Terachi, T., Siregar, U.J.and Miyashita, N.: John Wiley and Sons, Inc., Chichester. Use of RFLP analyzes for wheat germplasm evaluation. In : Damania, A.B.(ed.), Biodiversity and Wheat Improvement., 17-31 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tajima, F.Measurement of DNA polymorphism. In : Takahata, N.and Clark, A.G.(eds.): Japan Sci.Soc.Press, Tokyo/Sinauer Associates, Sunderland, MA.Mechanisms of Molecular Evolution., 37-59 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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