• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1994 Fiscal Year Final Research Report Summary

Studies on the microbiological basic technologies for bioremediation

Research Project

Project/Area Number 05304051
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 環境保全
Research InstitutionThe University of Tokyo

Principal Investigator

KODAMA Tohru  Univ.of Tokyo, Dept.of Biotechnol., Professor, 農学部, 教授 (30011901)

Co-Investigator(Kenkyū-buntansha) YAGI Osami  Nat.Inst.Environ., Div.of Water and Soil Env., Director, 水土壌圏環境部, 室長研究職 (40132865)
OHTAKE Hisao  Hirosima Univ., Dept.of Ferment.Technol., Professor, 工学部, 教授 (10127483)
FURUKAWA Kensuke  Kyushu Univ., Dept.OF Ferm., Professor, 農学部, 教授 (90221556)
KATO Nobuo  Kyoto Univ., Dept.of Agric.Chem., Professor, 農学部, 教授 (50026556)
IIJIMA Shinji  Nagoya Univ., Dept, of Biol.Func., Professor, 工学部, 教授 (00168056)
Project Period (FY) 1993 – 1994
KeywordsBioremediation / Genetical breeding / Bioreclamation of soils / Trichloroethylene / PCB degradation / Reduction of heavy metals / Denitrification / Degradation enzyme genes
Research Abstract

Through our research works performed during last year, the concept for "Bioremediation" was made clear, and the present state of the worldwide research development was fully understood. On the basis of these results, the microbiological research subjects for the practical bioremediation were investigated and discussed. Some of our members participated and presented the papers in the "OECD Workshop on Bioremediation Tokyo'94", held in November 1994. The effectiveness and safety as well as the necessity of international cooperation for the application of biotechnology for encironmental crises were discussed and confirmed at the workshop. Fundamental researches for the microbial degradation of BHC,chlorinated aromatic hydrocarbons, TCE,PEG and other recalcitrant compounds were developed. The degrading enzyme genes were analyzed and further investigated for enhancing the gene functions for the purpose of their practical use for bioremediation. Members belonging to private companies exploited the supporting technologies to establish the practical bioremediation, for example, improvement of the apparatus for effective treatment of polluted soil or waste water. The results of the present research works will be published by a publisher, Gihodo Shuppan Co.Ltd., in May 1995 in Japanese under the title "Various Kinds of Microorganisms Preserving the Environment".

  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Lee,J.-H.et al.: "Identification of the metabolic intermediates of phthalate by Tn5 mutant o Pseudomonas teststeroni and analysis of the 4,5-dihydroxyphthalate decarboxylase gene" J.Ferment.Bioeng.77. 583-590 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee,J.-H.et al.: "Identification of the plasmid-encoded 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase gene of Pseudomonas teststeroni involved in metabolism" J.Ferment.Bioeng.78. 462-465 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Konohana,T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawai,F.et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kita,K.et al.: "Degradation of poly (3-hydroxybutyrate),PHB,by marine bacteria and properties of PHB depolymerase from an isolate,Alcaligenes faecalis AE122" Appl.Environ.Microbiol.61. in press (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuchi,Y.et al.: "Identification of the bphA4 gene encoding ferredoxin reductase involved in the biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp.strain KKS102" J.Bacteriol.176. 1689-1694 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata,Y.et al.: "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase involced in the degradation of γ-hexachlorocyclohexane in Pseudomonas paucimobilis" J.Bacteriol.176. 3117-3125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda,M.et al.: "Identification of the bphyA and bphB genes of Pseudomonas sp.strain KKS102 involved in degradation of polychlorinated biphenyls" Biochem.Biophys.Res.Commun.202. 850-856 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Furukawa,K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.176. 2121-2123 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirose,J.et al.: "Construction of hybrid biphenyl (bph) and toluene (tod) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchiyama,H.et al.: "Immobilization of trichloroethylene-degrading bacterium,Methylocystis sp.strain M in different matrices" J.Ferment.Bioeng.77. 173-177 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Lee, J.-H.et al.: "Identification of the metabolic intermediates of phthalate by Tn 5 mutant of Pseudomonas teststeroni and analysis of the 4,5-dihydroxyphthalate decarboxylase gene" J.Ferment.Bioeng.77. 583-590 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Lee, J.-H.et al.: "Identification of the plasmid-encoded 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase gene of Pseudomonas teststeroni involved in metabolism" J.Ferment.Bioeng.78. 462-465 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Konohana, T.et al.: "Nitrate reduction in Bacillus licheniformis in the presence of ammonium salt by shaking culture" Biosci.Biotech.Biochem.57. 1082-1086 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawai, F.et al.: "Proposed mechanism for bacterial degradation of polyacrylate" J.Environ.Polym.Degr.2. 59-65 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kita, K.et al.: "Degradation of poly (3-hydroxybutyrate), PHB,by marine bacteria and properties of PHB depolymerase from an isolate, Alcaligenes faecalis AE122" Appl.Environ.Microbiol.61 (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuchi, Y.et al.: "Identification of the bphA4 gene encoding ferredoxin reductase involved in the hybrid and polychlorinated biphenyl degradation in Pseudomonas sp.strain KKS102" J.Bacteriol.176. 1689-1694 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, Y.et al.: "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase involced in the degradation of gamma-hexachlorocyclohexane in Pseudomonas paucimobilis" J.Bacteriol.176. 3117-3125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, M.et al.: "Identification of the bphA and bphB genes of Pseudomonas sp.strain KKS102 involved in degradation of polychlorinated biphenyls" Biochem.Biophys.Res.Commun.202. 850-856 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Furukawa, K.et al.: "Efficient degradation of trichloroethylene by a hybrid aromatic ring dioxygenase" J.Bacteriol.176. 2121-2123 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirose, J.et al.: "Construction of hybrid biphenyl (bph) and toluene (ted) genes for functional analysis of aromatic ring dioxygenases" Gene. 138. 27-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, J.et al.: "Cloning and characterization of a Pseudomonas aeruginosa gene involved in the negative regulation of phosphate taxis" J.Bacteriol.176. 5874-5877 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama, H.et al.: "Immobilization of trichloroethylene-degrading bacterium, Methylocystis sp.strain M in different matrices" J.Ferment.Bioeng.77. 173-177 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi