1995 Fiscal Year Final Research Report Summary
Development of gnotobiote mice for cancer research
| Project/Area Number |
05304059
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| Research Category |
Grant-in-Aid for Co-operative Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MITSUOKA Tomotari The Univ.of Tokyo, Fac.of Agriculture, Homorary Prof., 農学部, 名誉教授 (30157549)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Kazuhiro The Univ.of Tokyo, Fac.of Agriculture, Assist.Prof., 農学部, 助手 (60208858)
ITOH Kikuji The Univ.of Tokyo, Fac.of Agriculture, Assoc.Prof., 農学部, 助教授 (50100045)
HIOKI Kyouji Central Institute for Experimental Animals Research Head, 室長 (80208735)
ITOH Toshio Central Institute for Experimental Animals Research Head, 室長 (20106644)
NOMURA Tatsuji Central Institute for Experimental Animals, President, 所長 (10072399)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Gnotobiote / C-Ha-ras / DMH / Colon cancer / Bile acids / Transgenic mice |
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| Research Abstract |
Gnotobiotic transgenic mice with human cancer gene, c-Ha-ras gene, were Produced by oral inoculation with various intestinal bacteria isolated from human and mouse feces. We compared the colon tumor incidence after subcutaneous injection with dymetilhydrazin among various types of gnotobiotic mice. The tumor incidence of transgenic mice was higher than control mice in each group. The differences of floral composition affected the tumor incidence. In some cases floral bacteria stimulated the formation of tumors and in some cases inhibited. there were no relationship among tumor incidence, beta-glucuronidase activity and production of secondary bile acids.
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Research Products
(14 results)
