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1995 Fiscal Year Final Research Report Summary

Research on genetic variation and gene conservation of the tolerant poplar species for drought stress.

Research Project

Project/Area Number 05454077
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 林学
Research InstitutionThe University of Tokyo

Principal Investigator

IDE Yuji  The University of Tokyo, Faculty of Agriculture, Associate Professor, 農学部(林), 助教授 (90213024)

Co-Investigator(Kenkyū-buntansha) KOJIMA Katsumi  The University of Tokyo, Asian Natural Environmental Science Center, Associate P, アジア生物資源環境研究センター, 助教授 (80211895)
SASAKI Satohiko  The University of Tokyo, Faculty of Agriculture, Professor, 農学部, 教授 (20196158)
Project Period (FY) 1993 – 1995
KeywordsArid tolerance / Populus spp. / Tissue culture / Gene conservation
Research Abstract

Methods for clonal propagation by tissue culture, callus culture and protoplast culture were established in this research project. The reactions of poplar clones for the stresses of low water potential, salinity and low pH conditions were also investigated.
At first clonal propagation techniques of the poplars growing at the arid district in China was established. This made possible to conserve genetic resources of Chinese poplars which cannot grow in the field condition in Japan in vitro. This made the genetic conservation of temperate poplars possible too. The tolerance abilities for the stresses of low osmotic pressure and salinity and low pH of Populus euphratica, Populus tomentosa, Populus alba cv.pyramidalis X Populus tomentosa and Populus maximowiczii X Populus plantierensis were tested by using in vitro cultured shoots. Populus euphratica showed the highest tolerant for these three types of stress among test poplar clones. I think Populus euphratica has high genetic potential for the breeding of new cultivers for arid region. Finally protoplast culture was tested for these poplars. Successful colony formation was observed and the possibility of the genetic manipulation using protoplast culture was shown.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Ide,Y.: "In vitro plantlet regenration from petiole culture of Poplar hybrids and their competence for adventitious bud formation." Bull.Tokyo Univ.For.91. 127-135 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kang,J.-M.: "Isolation and culture of leaf protoplasts from in vitro subcultured poplars:Populus tomentosa, P.alba cv.pyramidalis X P.tomentosa and P.maximowiczii X P.plantierensis." Bull.Tokyo Univ.For.93. 59-63 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kang,J.-M.: "Growth responce to the stress of low water potential, salinity and high pH in cultured shoot of Chinese poplars." J.For.Res.1(in print). (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ide, Y.: "In vitro plantlet regeneration from petiole culture of Poplar hybrids and their competence for adventitious bud formation." Bull.Tokyo Univ.For.91. 127-135 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang, J.-M.: "Isolation and culture of leaf protoplasts from in vitro subcultured poplars : Populus tomentosa, P.alba cv.pyramidalis X P.tomentosa and P.maximowiczii X P.plantierensis." Bull.Tokyo Univ.For.93. 59-63 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kang, J.-M.: "Growth response to the stress of low water potential, salinity and high pH in cultured shoot of Chinese poplars." J.For.Res.1 (in print). (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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