1995 Fiscal Year Final Research Report Summary
Detection of novel molecules that regulates RB expression by screening random peptide library
| Project/Area Number |
05454178
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
TAKAHASHI Rei Kyoto Univ.Graduate School of Medicine Associate Professor, 医学研究科, 助教授 (60144565)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Toshiyuki Kyoto Prefectural Univ. of Medicine Research Associate, 医学部, 助手 (20186993)
SHIMADA Toshihide Kyoto Univ.Graduate School of Medicine Research Associate, 医学研究科, 助手 (30231690)
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| Project Period (FY) |
1993 – 1995
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| Keywords | peptide library / tumor suppressor gene / RB / p53 / promoter / enhancer |
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| Research Abstract |
Inactivation of the RB gene is one of the authentic models for carcinogenesis in tumor suppressor genes. Not only intragenic mutations but also inactivation of promoter activity by mutation or other epigenetic factors are of important factors that regulate expression of the RB gene. In this study, we try to elucidate mechanisms involved in such regulation of the promoter region of the RB gene by screening random peptides that were created by a phage library. we have isolated several clones which express peptide sequence binding to the RB promoter. The structural analysis including three dimensional construction will be performed to obtain structural basis for the interaction between the elements and the isolated peptide sequences. On the other hand, we also found a region in the RB promoter that can interact with a silencer molecule.
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