1995 Fiscal Year Final Research Report Summary
The role of prostatic interstitium for expression of the obstructive symptom and prostatic growth of benign prostatic hyperplasia
| Project/Area Number |
05454432
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | University of Tokyo |
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Principal Investigator |
MORIYAMA Nobuo The Univ.of Tokyo, Dept.of Urology, Assistant Professor, 医学部(病), 講師 (80143501)
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| Co-Investigator(Kenkyū-buntansha) |
KURIMOTO Shigeharu The Univ.of Tokyo, Dept.of Urology, Lecturer, 医学部(病), 助手 (30225271)
HORIE Shigeo National Cancer Center Hospital, Urologist, 医師 (40190243)
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| Project Period (FY) |
1993 – 1995
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| Keywords | Human prostate / Benign prostatic hyperplasia / alpha1-adrenoceptor / Rnase protection assay / in situ Hybridization |
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| Research Abstract |
The adrenergic stimulation induces the contraction of the hyperplastic tissues via a_l adrenoceptor and pharmacological studies suggested the existence of its subtypes. Recently three subtypes (a_<la>, a_<lb>, a_<ld>) have been detected and cloned using molecular biologic technique. Using the probes for these subtypes, we demonstrated their expression in cases with benign prostatic hypertplasia (BPH) and non-BPH tissues with in situ hybridization method. To quantify the ratio among these subtypes, RNase protection assay was also attempted in these cases. The expression of the a_<la> and a_<ld> adrenoceptors was diffusely reconginzed in the smooth muscles in the interstitium, while not in the glandular epithelial cells. On the contrary, the a_<lb> adrenoceptor was very weakly recognized. In all tissue samples tested, the predominant subtype mRNA was alpha1a. The total abundance of alpha1 mRNA in BPH samples was over six times that in non-BPH samples, the increased expression of alpha1a mRNA accounting for most of this increase (eight times that in non-BPH). The abundance of alpha1b did not vary between BPH and non-BPH samples, and the abundance of a ld in BPH samples was about three times in non-BPH samples. The ratio of the abundance of the subtype mRNAs, alpha1a : alpha1b : alpha1d was 63 : 8 : 29 in non-BPH samples and 85 : 1 : 14 in BPH samples.
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[Publications] Moriyama, N., Orikasa, E., Nakamura, T., Kurimoto, S., Ishida, Y.and Kawabe, K.: "Effect of bunazosin, alpha 1-adrenoceptor blocker, on multidirectional contractile response and localization of bunazosin binding sites in human hypertrophied prostate." Gen.Pharmacol.26. 1033-1037 (1995)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Moriyama, N., Kurimoto, S., Miata, N., Yamura, H., Yamazaki, R., Sudoh, K., Inagaki, O., Takenaka, T.and Kawabe, K.: "Decreased contractile effect of endothelin-1 on hyperplastic prostate." Gen.Pharmacol.(in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Moriyama, N., Kurimoto, S., Horie, S., Nasu, K., Tanaka, T., Yano, J., Hirano, H., Tsujimoto, G.and Kawabe, K.: "Detection of alpha1-Adrenoceptor subtypes in human hypertrophied prostate by in situ hybridization." Histochem.J.(in press).
Description
「研究成果報告書概要(欧文)」より
