1995 Fiscal Year Final Research Report Summary
Neuron circuit analysis on the basis of neuroactive substances and receptors
| Project/Area Number |
05454658
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
MIZUNO Noboru Kyoto Univ., Fac.of Med., Prof., 医学研究所, 教授 (10025596)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Sakashi Kyoto Unoiv., Coll.Med.Technol., Prof., 医療技術短期大学部, 教授 (60034188)
TOKUNO Hironobu Kyoto Univ., School of Med., Res.Assoc., 医学研究科, 助手 (40212071)
TAKADA Masahiko Kyoto Univ., School of Med., Lecturer, 医学研究科, 講師 (00236233)
KANEKO Takeshi Kyoto Univ., School of Med., Assoc.Prof., 医学研究科, 助教授 (90177519)
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| Project Period (FY) |
1993 – 1995
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| Keywords | cerebral cortex / striatum / cerebellum / pain / endogenous paincontrol system / opioid receptor / metabotropic glutamate receptor / substance P receptor |
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| Research Abstract |
1.An antibody against substance P receptor (SPR) was raised, and distribution of SPR in the central nervous system (CNS) of the rat was examined immunohistochemically. The vast majority of cerebral cortical neurons with SPR were GABAergic non-pyramidal neurons. Striatal neurons with SPR were segregated into somatostatinergic and cholinergic apiny (intrinsic) neurons.
2.Distributions of mRNAs for metabotropic glutamate receptors, mGluR2-mGluR7 in the rat CNS were examined by in situ hybridization. Antibodies against mGluR2, and mGluR4 - mGluR8 were raised. Presynaotic localization of m GluRs were immunohistochemically observed light and electron microscopically in the cerebellar cortex (mGluR2 and mGluR4) , layr I of the spinal cord (mGluR7) and olfactory tubercle (mGluR8).
3.Two kinds of antibodies against mu-opioid receptor (MOR) were raised, and distribution of MOR in the rat CNS was examined immunohistochemically. Presynaptic localization of MOR was observed light and electron microscopically in the medial terminal nucleus of the accessory optic tract, nucleus of the solitary tract, and layr I of the spinal cord. In layr I of the spinal cord, some axon terminalswith substance P contained MOR.
4.Analysis was performed on the local circuitry in the cerebral cortex, striatum and cerebellar cortex, the nociceptive system, the endogeous pain-control system, and masticatory system.
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