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1995 Fiscal Year Final Research Report Summary

GENE TRANSFORMATION EXPERIMENT OF THE SELF-INCOMPATIBILITY RELATED GENES

Research Project

Project/Area Number 05556002
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

HINATA Kokichi  TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (00005589)

Co-Investigator(Kenkyū-buntansha) WATANABE Masao  TOHOKU UNIVERSITY,FAC.OF AGRICULTURE,ASSIS.RESEARCHER., 農学部, 助手 (90240522)
TORIYAMA Kinya  TOHOKU UNIVERSITY,FAC.OF AGRICULTURE,ASSOC.PROFESSOR., 農学部, 助教授 (20183882)
NAKANISHI Tetsu  KOBE UNIVERSITY,DEPARTMENT OF NATURAL SCIENCE,PROFESSOR., 大学院・自然科学研究科, 教授 (80031227)
SAKIYAMA Fumio  OSAKA UNIVERSITY,INSTITUTE FOR PROTEIN RESEARCH,PROFESSOR., 蛋白質研究所, 教授 (40029947)
ISOGAI Akira  NARA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY,GRADUATE SCHOOL OF BIOLOGICAL, バイオサイエンス研究科, 教授 (20011992)
Project Period (FY) 1993 – 1995
KeywordsSELF-INCOMPATIBILITY / BRASSICA CAMPESTRIS / PYRUS PILIFOLIA / SLG / SRK / S-RNASE / GENE-MANIPULATION / TRANSGENIC PLANT
Research Abstract

Objectives : To understand the molecular mechanism of self-incompatibility and to assess a possibility of molecular breeding of this character, molecular characterization and gene manipulation was conducted on the S-related genes in sporophytic self-incompatible plant : Brassica campestris and in gemetophytic self-incompatible plant : Pyrus pilifolia.
Results : In B.campestris, SLG8, SLG9, SLG12, SRK8, SRK9, SRK12, SRA1, SRA2, SRA3, SRA4 and SRBs were isolated from cDNA or genomic DNA libraries and sequenced. Dendrograms constructed on the basis of synonymous and nonsynonymous substitutions indicated that SRA differentiated first, followed by SRB and SLG/SRK.When the SLG8 genomic clone was introduced into B.campestris some of transformed plants changed to self-compatible. Furthermore, the antisense SLG gene was transformed into the same species. Analysis of a transformant showed that the self-incompatibility was broken down by the action of the introduced antisense gene. These results i … More ndicated the self-incompatibility could be controlled, at least partly, by gene manipulation. The genes encoding 3 RNases were cloned from the style of a self-incompatible cultivar, Nijisseiki (S2S4), and its self-compatible mutant, Osa-Nijisseiki (S2S4sm, sm means stylar part mutant), of Japanese pear. For Nijisseiki, cDNAs coding for two S-RNases (S2-RNase and S4-RNase) and an RNase unrelated to self-incompatibility (non-S-RNase) were cloned from the stylar cDNA library. However, the cDNA coding for S4-RNase was neither amplified by PCR nor cloned from the library of Osa-Nijisseiki, suggesting that the mutation of self-incompatible Nijisseiki to self-compatible Osa-Nijisseiki is due to a failure of expression of S4-RNase. The S3-gene promoter of Petunia inflata was used in constructing the vectors for the Japanese pear S-genes. The binary vectors of the Agrobacterium were transformed in the petunia resulting in shoot regeneration. In vitro cultures of the Japanese pear were also extended. Less

  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Yamakawa, S., Watanabe, M., Isogai, A., Takayama, S., Satoh, S., Hinata, K. and Suzuki, A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 173-175 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nou, I. S., Watanabe, M., Isuzugawa, K., Isogai, A. and Hinata, K.: "Isolation of S-allele from an wild population of Brassica compestris L. at Balcesme, Turkye and their characterization by S-glycoprotein." Sex. Plant Reprod.6. 71-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nou, I. S., Watanabe, M., Isogai, A. and Hinata, K.: "Comparision of S-alleles and S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex. Plant Reprod.6. 79-86 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hinata, K., Watanabe, M., Toriyama, k. and Isogai, A.: "A review of recent studies on homomorphic self-incompatibility." Inter. Rev. Cytol.143. 257-296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex. Plant Reprod. 7. 7. 107-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in distylous Averrhoa carambola L." Sex. Plant Reprod.7. 116-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A. and Suzuki, A.: "The sequence of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci. Biotech. Biochem.58. 921-925 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe, M., Takasaki, T., Toriyama, K., Yamakawa, S., Isogai, A., Suzuki, A. and Hinata, K.: "A high degree of homology exists between the protein encoded by SLG and the S recept or domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol.35. 1221-1229 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hinata, K., Watanabe, M., Isogai, A., Yamakawa, S., Satta, Y. and Takahata, N.: "Genetic studies of self-incompatible Brassica campestris." “Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.). 12. 103-110 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsushita, M., Watanabe, M., Yamakawa, S., Isogai, A. and Hinata, K.: "Base sequence variation of the S-glycoprotein within the same S12-allele in Brassica campestris." "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.). 12. 313-315 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakawa, S., Watanabe, M., Hinata, K., Suzuki, A. and Isogai, A.: "The sequences of S-receptor kinase (SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris." Biosci. Biotech. Biochem.59. 161-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hinata, K., Watanabe, M., Yamakawa, S., Satta, Y. and Isogai, A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system : synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A. and Hinata, K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinba, H., Hinata, K., Suzuki, A. and Isogai, A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc. Japan Academy,. 71B. 81-83 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuroda, S., Norioka, S., Mitta, M., Kato, I., and Sakiyama, F.: "Primary structure of a novel stylar RNaseunassociated with self-incompatibility in tobacco plant, Nicotiana alata." J. Protein Chem.13. 438-439 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishimizu, T., Miyagi, M., Norioka, S., Ying-Hong Liu, Clarke, A. E. and Sakiyama, F.: "Identification of histidine 31 and cysteine 95 in the active site of self-incompatibitlity associated S6-RNase in Nicotiana alata." J. Biochem.118. 1007-1013 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Norioka N., Ohnishi Y., Norioka S., Ishimizu T., Nakanishi, T. & Sakiyama F.: "Nucleotide Sequences of cDNAs Encodinf S2-and S4-RNases from Japanese Pear (Pyurs pyrifolia Nakai)" Plant Physiol.108. 1343 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺正夫・鳥山欽哉: "花粉学事典(日本花粉学会編,分担執筆)" 朝倉書店, (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hinata, K., Isogai, A. and Isuzugawa, K.,: "Genetic control of self-incompatibility and reproductive development in flowering plants" eds. by Williams, E. G., Clarke, A. E. and Knox, R. B.," Kluwer Academic Publishers,102-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamakawa, S., Watanabe, M., Isogai, A., Takayama, S., Satoh, S., Hinata, K.and Suzuki, A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins" Plant Cell Physiol. 34(1). 173-175 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nou, I.S., Watanabe, M., Isuzugawa, K., Isogai, A.and Hinata, K.: "Isolation of S-allele from a wild population of Brassica campestris L.at Balcesme, Turkey and their characterization by S-glycoprotein." Sex. Plant Reprod. 6(1). 71-78 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nou, I.S., Watanabe, M., Isogai, A.and Hinata, K.: "Comparison of S-alleles S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex. Plant Reprod. 6(2). 79-86 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hinata, K., Watanabe, M., Toriyama, K.and Isogai, A.: "A review of recent studies on homomorphic self-incompatibility." Intern. Rev. Cytol. 143. 257-296 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wong, K.C., Watanabe, M.and Hinata, K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex. Plant Reprod. 7(2). 107-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wong, K.C., Watanabe, and Hinata, K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in distylous Averrhoa carambola L." Sex. Plant Reprod. 7(2). 116-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A.and Suzuki, A.: "The sequence of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci. Biotech. Biochem. 58(5). 921-925 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, M., Takasaki, T., Toriyama, K., Yamakawa, S., Isogai, A., Suzuki, A.and Hinata, K.: "A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol. 35(8). 1221-1229 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hinata, K., Watanabe, M., Isogai, A., Yamakawa, S., Satta, Y.and Takahara, N.: "Genetic studies of self-incompatible Brassica campestris" In "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.)". 12. 103-110 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsushita, M., Watanabe, M., Yamakawa, S., Isogai, A., and Hinata, K.: "Base sequence variation of the S-glycoprotein within the same S12-allele in Brassica campestris." In "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.)". 12. 313-315 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamakawa, S., Watanabe, M., Hinata, K., Suzuki, A.and Isogai, A.: "The sequences of S-receptor kinase (SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris" Biosci. Biotech. Biochem.59(1). 161-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hinata, K., Watanabe, M., Yamakawa, S., Satta, Y., and Isogai, A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system : synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A.and Hinata, K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol. 36(7). 1273-1280 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hinata, K., Isogai, A.and Isuzugawa, K.: "Manipulation of sporophytic self-incompatibility in plant breeding" In "Genetic control of self-incompatibility and reproductive development in flowering plants" eds. by Williams, E.G., Clarke, A.E.and Knox, R.B., Kluwer Academic Publishers. 540. 102-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shiba, H., Hinata, K., Suzuki, A.and Isogai, A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc. Japan Academy. 71, Sr. B. 81-83 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuroda, S., Norioka, S., Mitta, M., Kato, I., and Sakiyama, F.: "Primary structure of a novel stylar RNaseunassociated with self-incompatibility in tobacco plant, Nicotiana alata" J.Protein Chem. 13. 438-439 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishimizu, T., Miyagi, M., Norioka, S., Ying-Hong Liu, Clarke, A.E.and Sakiyama, F.: "Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility associated S6-RNase in Nicotiana alata" J.Biochem. 118. 1007-1013 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Norioka, N., Ohnishi, Y., Norioka, S., Ishimizu, T., Nakanishi, T.and Sakiyama F.: "Nucleotide Sequences of cDNAs Encodinf S2-and S4-RNases from Japanese Pear (Pyrus pyrifolia Nakai)" Plant Physiol. 108. 1343 (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1997-03-04  

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