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1995 Fiscal Year Final Research Report Summary

The development of viral vector for in vivo gene-delivery and its applications

Research Project

Project/Area Number 05558094
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionWakayama Medical College

Principal Investigator

SENBA Emiko  Wakayama Medical College, Faculty of Medicine, Professor, 医学部, 教授 (00135691)

Co-Investigator(Kenkyū-buntansha) KAWAI Yoshinori  Wakayama Medical College, Faculty of Medicine, Lecturer, 医学部, 講師 (80211861)
TONE Shigenobu  Tokyo Metropolitan Instittute for Clinical Medicine, Dept.of Radiological Medici, 放射線医学研究部門, 主任研究員 (70211399)
MIYAMOTO Hiroyuki  Wakayama Medical College, Faculty of Medicine, Professor, 医学部, 教授 (90029778)
Project Period (FY) 1993 – 1995
Keywordspseudorabies virus / transneuronal tracer / gene-delivery / masseter / motor trigeminal nucleus / mesencephalic trigeminal nucleus / retrograde transport
Research Abstract

Fusion gene of non-essential glycoprotein gG (gX) and lacZ was constructed and pseudorabies virus (PrV) mutants were produced. Characteristics and usefulness of this mutant virus as a transneuronal tracer was investigated by injecting it into masseter or stomach of SD rats. Injection of PrV solution with titer of 1.0*10^7p.f.u./ml was most suitable for the tracing study. Injection of higher titer of viral solution killed the animals within 2 days, indicating the retained virulence of this mutant virus. Although viral infection occurred in motor trigeminal n.neurous in all cases of masseter injection, labeled neurous in the mesencephalic trigeminal n.were very few. Second-or third-order neurons were identified in the locus coeruleus (LC), raphe magnus (RM), medullary reticular formation (IRt), Edinger-Westphal nucleus (EW), tuberal magnocellular n.(TM), lateral hypothalamic n.(LH) and paraventricular hypothalamic n.(PVH). No anterograde tracing was detected in the CNS.Injection of this virus into stomach labeled neurons in the dorsal motor nucleus of N.X (DMN), n.tractus solitarius, n. ambiguus, A5 and PVH.These findings indicate that this virus preferentially infecct somatic and visceral motoneurons and is retrogradely tranported in second-order or third-order neurons in the CNS.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Kashiba H.et al.: "Relationship betweenBDNF- and Trk-expressing neurons in rat dorsal root ganglion:an analysis by in situ hybridization" Neuroreport. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Senba E. & Kashiba H.: "Sensory afferent procssing in multiresponsive DRG neurons" Progress in Brain Research. 113. 387-410 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nemoto K.et al.: "Mutation of low affinity nerve growth factor receptor gene is associated with the hypertensive phenotype in SHR." Neurosci.Lett.210. 69-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba H.et al.: "Coexpression of preprotachykinin A(PPTA),a-calcitonin gene related peptide (CGRP),somatostatin and neurotrophin receptor・・・" Neuroscience. 70. 179-189 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noguchi K.et al.: "Substance P induced by peripheral nerve injury in primary afferent sensory neurons and its effect on dorsal column・・・・" Journal of Neuroscience. 15. 7633-7643 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokunaga A.et al.: "Effects of local anesthesia on formalin-induced Fos expression in the rat dorsal horn." Neuroreport. 30. 158-164 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba, H., Nemoto, K.Ueyama, T.and Senba E.: "Relationship between BDNF- and Trk-expressing neurons in rat dorsal root ganglion : an analysis by in situ hybridization." Neuroreport. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, H., Ueda, Y.and Senba, E.: "Systemic capsaicin in the adult rat differentially affects gene expression for neuropeptides and neurotropnin recptors in primaly sensory neurons." Neuroscience. 76. 299-312 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Senba, E.and Kashiba, H.: "sensory afferent processing in multiresponsive DRG neurons" Progr.Brain Res. 113. 387-410 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kageyama, H., Nemoto, K., Nemoto, F., Sekimoto, M., Nara, Y., Nabika, T., Iwayama, Y., Fukamachi, K., Tomita, I., Senba, E., Forehand, C.J., Hendley, E.D., and Ueyama, T.: "Mutation of the trkB gene encoding the high-affinity receptor for brain-derived neurotrophic factor in stroke-prone spontaneously hypertensive rats." Biochem.Biophysic.Res.Comm.229. 713-718 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nemoto, K., Kageyama, H., Ueyama, T., Fukamachi, K., Sekimoto, M.Tomita, I,Senba, E,Forehand, C.J.and Hendley, E.D.: "Mutation of low affinity nerve growth factor receptor gene is associated with the hypertensive phenotype in spontaneously hypertensive inbred rat strains" Neurosci.Lett.210. 69-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, H., Ueda, Y.and Senba, E.: "Coexpression of preprotachykinin A (PPTA), a-calcitonin gene related peptide (a-CGRP), somatostatin (SOM) and neurotrophin receptor family mRNAs in rat dorsal root ganglion neurons." Neuroscience. 70. 179-189 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, K., Kawai, Y., Fukuoka, T., Senba, E.and Miki, K.: "Substance P induced by peripheral nerve injury in primary afferent sensory neurons and its effect on dorsal column nucleus neurons." J.Neurosci.15. 7633-7643 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokunaga, A., Doi, M.and Senba, E.: "(1995) Effects of local anesthesia on formalin-induced Fos expression in the rat dorsal horn." Neuroreport. 6. 2301-2304 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, H., Noguchi, K., Ueda, Y.and Senba, E.: "Coexpression of trk family members and low-affinity neurotrophin receptors in rat dorsal root ganglion neurons." Mol.Brain.Res.30. 158-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umemoto, S., Noguchi, K., Kawai, Y., and Senba, E.: "The expression of neuropeptides and their mRNAs in the trigeminal mesencephalic nucleus following masseteric nerve transection." Mol.Brain.Res.23. 93-99 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, H., Noguchi, K., Ueda, Y.and Senba E.: "Neuropeptide Y and galanin are coexpressed in rat large type A sensory neurons after peripheral transection" Peptides. 15. 411-416

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, K., Dubner, R., De Leon, M., Senba, E.and Ruda, M.A.: "(1994) Axotomy induces preprotachykinin gene expression in a subpopulation of dorsal root ganglion neurons." J.Neurosci.Res.37. 596-603 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noguchi, K., De Leon, M., Nahin, R.L., Senba, E.and Ruda, M.A.: "Quantification of axotomy-induced alteration of neuropeptide mRNAs in dorsal root ganglion neurons with special reference to neuropeptide Y mRNA and the effects of neonatal capsaicin treatment" J.Neurosci.Res.35. 54-66 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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